scholarly journals PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

2001 ◽  
Vol 35 (2) ◽  
pp. 157-162 ◽  
Author(s):  
F. Benavides ◽  
E. Glasscock ◽  
L. G. Coghlan ◽  
M. C. Stern ◽  
D. A. Weiss ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Sequence Length ◽  
Mouse Strains ◽  
Mechanistic Studies ◽  
Chain Reaction ◽  
Dna Microsatellites ◽  
Multi Stage ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
Simple Sequence

Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

A genetic map of the mouse suitable for typing intraspecific crosses.

Genetics ◽  
1992 ◽  
Vol 131 (2) ◽  
pp. 423-447 ◽  
Author(s):  
W Dietrich ◽  
H Katz ◽  
S E Lincoln ◽  
H S Shin ◽  
J Friedman ◽  
...  
Keyword(s):  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Mouse Genome ◽  
Sequence Length ◽  
Chain Reaction ◽  
Average Spacing ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
High Degree ◽  
Simple Sequence

Abstract We report the construction of a genetic linkage map of the mouse, consisting entirely of genetic markers that can be rapidly typed by polymerase chain reaction and that show a high degree of polymorphism among inbred laboratory strains. Specifically, the map contains 317 simple sequence length polymorphisms at an average spacing of 4.3 cM and is detectably linked to approximately 99% of the mouse genome. In typical crosses between inbred laboratory strains, about 50% of the markers are polymorphic, making it straightforward to follow inheritance in almost any cross.

scholarly journals Molecular markers and their application for DNA fingerprinting and genetic diversity studies in Coffea species Marka molekuler dan penerapannya untuk studi sidik jari DNA dan keragaman genetik pada spesies Coffea

2016 ◽  
Vol 82 (1) ◽  
Author(s):  
. PRIYONO ◽  
Riza Arief PUTRANTO
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Dna Fingerprinting ◽  
Fragment Length ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Polymerase Chain ◽  
Simple Sequence

AbstrakStrategi klasik yang meliputi perbandingan anatomi, fisiologi dan sitogenetika telah banyak diterapkan untuk mengidentifikasi karakter tertentu serta untuk menentukan keragaman dan hubungan antar dan intra spesies. Namun, saat ini penanda molekuler telah melengkapi strategi sebelumnya dengan sangat cepat. Berbagai jenis penanda molekuler digunakan untuk menilai tingkat polimorfisme DNA. Penanda molekuler ini diklasifikasikan sebagai penanda berbasis hibridisasi dan berbasis Polymerase Chain Reaction (PCR). Dalam beberapa tahun terakhir, sistem penanda DNA yang berbeda seperti Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) yang juga disebut Mikrosatelit, Single Nucleotide Polymorphims (SNPs) dan lain-lain telah dikembangkan dan diterapkan pada berbagai spesies tanaman. Penanda molekuler ini dapat digunakan untuk sidik jari DNA dan studi keragaman genetik. Sidik jari berdasarkan DNA telah banyak digunakan dalam ilmu forensik, juga memiliki berbagai aplikasi dalam pemuliaan tanaman. Tulisan ini memberikan overview tentang berbagai penanda molekuler dan aplikasinya untuk sidik jari dan kajian keragaman genetik tanaman berdasarkan DNA pada berbagai spesies tanaman, dan secara khusus pada Coffea sp.AbstractConventional strategies including comparative anatomy, physiology and cytogenetics were applied to identify the certain character as well as to determine inter- and intra-species diversity and relationships. However, more recently molecular markers have very rapidly complemented the previous strategies. Various types of molecular markers are used to assess DNA polymorphism. They are classified as hybridization-based markers and polymerase chain reaction (PCR) based markers. In recent years, different DNA marker systems such as Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) which also called as microsatellites, Single Nucleotide  Polymorphims  (SNPs)  and  others  have been developed and applied to a range of plant species. These molecular markers can be used for DNA fingerprinting and genetic diversity study. DNA fingerprinting has been widely used in forensic science, but is has also a variety of application in plant breeding. This paper provides an overview about various molecular markers and their application for DNA plant fingerprinting and genetic diversity, especially in Coffea sp.

scholarly journals Molecular markers and their application for DNA fingerprinting and genetic diversity studies in Coffea species Marka molekuler dan penerapannya untuk studi sidik jari DNA dan keragaman genetik pada spesies Coffea

2016 ◽  
Vol 82 (1) ◽  
Author(s):  
. PRIYONO ◽  
Riza Arief PUTRANTO
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Dna Fingerprinting ◽  
Fragment Length ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Polymerase Chain ◽  
Simple Sequence

AbstrakStrategi klasik yang meliputi perbandingan anatomi, fisiologi dan sitogenetika telah banyak diterapkan untuk mengidentifikasi karakter tertentu serta untuk menentukan keragaman dan hubungan antar dan intra spesies. Namun, saat ini penanda molekuler telah melengkapi strategi sebelumnya dengan sangat cepat. Berbagai jenis penanda molekuler digunakan untuk menilai tingkat polimorfisme DNA. Penanda molekuler ini diklasifikasikan sebagai penanda berbasis hibridisasi dan berbasis Polymerase Chain Reaction (PCR). Dalam beberapa tahun terakhir, sistem penanda DNA yang berbeda seperti Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) yang juga disebut Mikrosatelit, Single Nucleotide Polymorphims (SNPs) dan lain-lain telah dikembangkan dan diterapkan pada berbagai spesies tanaman. Penanda molekuler ini dapat digunakan untuk sidik jari DNA dan studi keragaman genetik. Sidik jari berdasarkan DNA telah banyak digunakan dalam ilmu forensik, juga memiliki berbagai aplikasi dalam pemuliaan tanaman. Tulisan ini memberikan overview tentang berbagai penanda molekuler dan aplikasinya untuk sidik jari dan kajian keragaman genetik tanaman berdasarkan DNA pada berbagai spesies tanaman, dan secara khusus pada Coffea sp.AbstractConventional strategies including comparative anatomy, physiology and cytogenetics were applied to identify the certain character as well as to determine inter- and intra-species diversity and relationships. However, more recently molecular markers have very rapidly complemented the previous strategies. Various types of molecular markers are used to assess DNA polymorphism. They are classified as hybridization-based markers and polymerase chain reaction (PCR) based markers. In recent years, different DNA marker systems such as Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) which also called as microsatellites, Single Nucleotide  Polymorphims  (SNPs)  and  others  have been developed and applied to a range of plant species. These molecular markers can be used for DNA fingerprinting and genetic diversity study. DNA fingerprinting has been widely used in forensic science, but is has also a variety of application in plant breeding. This paper provides an overview about various molecular markers and their application for DNA plant fingerprinting and genetic diversity, especially in Coffea sp.

The SUSPECT SYMPTOMS OF INBREEDING DEPRESSION AND THE HOMOZYGOSITY LEVEL OF FOURTH GENERATION OF SP450T AND FIFTH GENERATION OF DURA DELI OIL PALM SELFING POPULATION

2018 ◽  
Vol 24 (2) ◽  
pp. 55-66
Author(s):  
Rokhana Faizah ◽  
Sri Wening ◽  
Hernawan Yuli Rahmadi ◽  
Abdul Razak Purba
Keyword(s):  
Inbreeding Depression ◽  
Oil Palm ◽  
Recurrent Selection ◽  
Fourth Generation ◽  
Depression Symptoms ◽  
Reciprocal Recurrent Selection ◽  
Chain Reaction ◽  
Fifth Generation ◽  
Polymerase Chain ◽  
Simple Sequence

Inbreeding is a common method used to reproduce candidate mother plant from selected parental lines for commercial seeds in Reciprocal Recurrent Selection (RRS) oil palm breeding program. However this practice may increased homozigosity level of selected population. This study concerned the level of homozygosity of SP540T fourth generations and Dura Deli Dolok Sinumbah fifth generations (3 crosses respectively) and their correlation with inbreeding depression symptoms. Polymerase Chain Reaction-Simple Sequence Repeat (PCR-SSR) with 16 markers developed for oil palm was used to analyze 327 samples. The result shows that the levels of homozigosity of SP540T fourth selfing generation were ranged between 0.44-0.84 or 0.61 in average. While the levels of homozygosity of Dura Deli fifth selfing generations were ranged between 0.60-0.93 or 0.78 in average. The homozygosity level in Dura Deli was 1.27% higher than SP540T populations. Correlation analysis showed that the higher the level of homozygosity, the higher of the inbreeding symptoms 2 observed (R =0.95).

Use of simple sequence length polymorphisms for genetic characterization of rat inbred strains

1995 ◽  
Vol 6 (9) ◽  
pp. 595-601 ◽  
Author(s):  
M. Otsen ◽  
M. Den Bieman ◽  
E. S. Winer ◽  
H. J. Jacob ◽  
J. Szpirer ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Inbred Strains ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Simple Sequence

A genetic map of the mouse with 4,006 simple sequence length polymorphisms

1994 ◽  
Vol 7 (S2) ◽  
pp. 220-245 ◽  
Author(s):  
William F. Dietrich ◽  
Joyce C. Miller ◽  
Robert G. Steen ◽  
Mark Merchant ◽  
Deborah Damron ◽  
...  
Keyword(s):  
Genetic Map ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Simple Sequence
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