Use of simple sequence length polymorphisms for genetic characterization of rat inbred strains

1995 ◽  
Vol 6 (9) ◽  
pp. 595-601 ◽  
Author(s):  
M. Otsen ◽  
M. Den Bieman ◽  
E. S. Winer ◽  
H. J. Jacob ◽  
J. Szpirer ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Inbred Strains ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Simple Sequence

Simple sequence length polymorphisms (SSLPs) that distinguish MOLF/Ei and 129/Sv inbred strains of laboratory mice

1998 ◽  
Vol 9 (8) ◽  
pp. 668-670 ◽  
Author(s):  
Angabin Matin ◽  
Gayle B. Collin ◽  
Yoshinobu Asada ◽  
Don Varnum ◽  
Diana L. Martone ◽  
...  
Keyword(s):  
Inbred Strains ◽  
Sequence Length ◽  
Laboratory Mice ◽  
Length Polymorphisms ◽  
Simple Sequence

scholarly journals The Arabidopsis ALDP protein homologue COMATOSE is instrumental in peroxisomal acetate metabolism

2007 ◽  
Vol 406 (3) ◽  
pp. 399-406 ◽  
Author(s):  
Mark A. Hooks ◽  
James E. Turner ◽  
Elaine C. Murphy ◽  
Katherine A. Johnston ◽  
Sally Burr ◽  
...  
Keyword(s):  
Glyoxylate Cycle ◽  
Sequence Length ◽  
Acetate Metabolism ◽  
Soluble Carbohydrate ◽  
Wild Type ◽  
Abc Protein ◽  
Atp Binding Cassette Transporter ◽  
Mutant Lines ◽  
Simple Sequence

The Arabidopsis acn (acetate non-utilizing) mutants were isolated by fluoroacetate-resistant germination and seedling establishment. We report the characterization of the acn2 mutant. Physiological analyses of acn2 showed that it possessed characteristics similar to those of the mutants cts (COMATOSE)-1 and pxa [peroxisomal ABC (ATP-binding-cassette) transporter]1. The acn2 locus was mapped to within 3 cM of the CTS gene on the bottom arm of chromosome IV using CAPS (cleavage amplification polymorphism) and SSLP (simple sequence-length polymorphism) markers. Crossing acn2 and cts-1 failed to restore the fluoroacetate-sensitive phenotype, suggesting that these mutations were allelic. Sequencing of the ACN2 locus revealed a C→T nonsense mutation in exon 13, which would have resulted in the elimination of the C-terminal hemitransporter domain of the encoded protein. Neither the full-length CTS protein nor the truncated protein was detected on immunoblots using either C-terminal- or N-terminal-specific anti-CTS antibodies respectively, demonstrating the absence of the entire CTS protein in acn2 mutants. Emerged seedlings of both cts-1 and pxa1 alleles displayed increased resistance to FAc (monofluoroacetic acid) compared with the corresponding wild-type seedlings. Complementation studies showed that mutation of the CTS gene was responsible for the FAc-resistant phenotype, as when the wild-type protein was expressed in both the cts-1 and pxa1 mutant lines, the strains became FAc-sensitive. Feeding studies confirmed that both acn2 and cts-1 mutants were compromised in their ability to convert radiolabelled acetate into soluble carbohydrate. These results demonstrate a role for the ABC protein CTS in providing acetate to the glyoxylate cycle in developing seedlings.

A genetic map of the mouse with 4,006 simple sequence length polymorphisms

1994 ◽  
Vol 7 (S2) ◽  
pp. 220-245 ◽  
Author(s):  
William F. Dietrich ◽  
Joyce C. Miller ◽  
Robert G. Steen ◽  
Mark Merchant ◽  
Deborah Damron ◽  
...  
Keyword(s):  
Genetic Map ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Simple Sequence

Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 715-723 ◽  
Author(s):  
P. J. Maughan ◽  
M. A. Saghai Maroof ◽  
G. R. Buss
Keyword(s):  
Genetic Markers ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Allelic Diversity ◽  
Sequence Length ◽  
Length Polymorphisms ◽  
Ssr Loci ◽  
Low Levels ◽  
Cultivated Soybean ◽  
Simple Sequence

The objectives of this study were to (i) assess the extent of genetic variation in soybean microsatellites (simple sequence repeats or SSRs), (ii) assay for amplified sequence length polymorphisms (ASLPs), and (iii) evaluate the usefulness of SSRs and ASLPs as genetic markers. Five microsatellites detected a total of 79 variants (alleles) in a sample of 94 accessions of wild (Glycine soja) and cultivated soybean (G. max). F2 segregation analysis of four of the five microsatellites identified these variants (alleles) with four loci located in independent linkage groups. The number of alleles per microsatellite locus ranged from 5 to 21; to our knowledge these are the largest numbers of alleles for single Mendelian loci reported in soybean. Allelic diversity for the SSR loci was greater in wild than in cultivated soybean. Overall, 43 more SSR alleles were detected in wild than in cultivated soybean. These results indicate that SSRs are the marker of choice, especially for species with low levels of variation as detected by other types of markers. Two alleles were detected at each of the three ASLP loci examined. A total of six ASLP alleles were observed in cultivated soybean and five were observed in wild soybean; all alleles detected in wild soybean were present in cultivated soybean. Allelic diversity values for the ASLP loci were near previous estimates for restriction fragment length polymorphisms and therefore ASLPs may be useful as genetic markers in site-directed mapping.Key words: microsatellite, simple sequence repeat, soybean, amplified sequence length polymorphism, genetic mapping.

Pathotype Complexity and Genetic Characterization of Phytophthora sojae Populations in Illinois, Indiana, Kentucky, and Ohio

2021 ◽  
Author(s):  
Linda Weber Hebb ◽  
Carl A. Bradley ◽  
Santiago Xavier Mideros ◽  
Darcy E. P. Telenko ◽  
Kiersten Wise ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Resistance Genes ◽  
Genetic Characterization ◽  
Specific Resistance ◽  
Agricultural Practices ◽  
Phytophthora Sojae ◽  
Simple Sequence Repeat Markers ◽  
Rps Gene ◽  
Simple Sequence

Phytophthora sojae, the causal agent of Phytophthora root and stem rot of soybean, has been managed with single Rps genes since the 1960’s, but has subsequently adapted to many of these resistance genes, rendering them ineffective. The objective of this study was to examine the pathotype and genetic diversity of P. sojae from soil samples across Illinois, Indiana, Kentucky, and Ohio by assessing which Rps gene(s) were still effective and identifying possible population clusters. There were 218 pathotypes identified from 473 P. sojae isolates with an average of 6.7 out of 15 differential soybean lines exhibiting a susceptible response for each isolate. Genetic characterization of 103 P. sojae isolates from across Illinois, Indiana, Kentucky, and Ohio with 19 simple sequence repeat markers identified 92 multilocus genotypes. There was a moderate level of population differentiation among these four states, with pairwise FST values ranging from 0.026 to 0.246. There was also moderate to high levels of differentiation between fields, with pairwise FST values ranging from 0.071 to 0.537. Additionally, cluster analysis detected the presence of P. sojae population structure across neighboring states. The level of pathotype and genetic diversity, in addition to the identification of population clusters, supports the hypothesis of occasional outcrossing events that allow for an increase in diversity and the potential to select for a loss in avirulence to specific resistance genes within regions. The trend of suspected gene flow among neighboring fields is expected to be an ongoing issue with current agricultural practices.

scholarly journals PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

2001 ◽  
Vol 35 (2) ◽  
pp. 157-162 ◽  
Author(s):  
F. Benavides ◽  
E. Glasscock ◽  
L. G. Coghlan ◽  
M. C. Stern ◽  
D. A. Weiss ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Sequence Length ◽  
Mouse Strains ◽  
Mechanistic Studies ◽  
Chain Reaction ◽  
Dna Microsatellites ◽  
Multi Stage ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
Simple Sequence

Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

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