Comparison of alkaline phosphatase and horseradish peroxidase conjugated antisera in the ELISA test for antibodies to reovirus 3, mouse hepatitis and Sendai viruses

1981 ◽  
Vol 15 (1) ◽  
pp. 69-74 ◽  
Author(s):  
P. Carthew ◽  
J. Gannon ◽  
I. Whisson
Keyword(s):  
Alkaline Phosphatase ◽  
Horseradish Peroxidase ◽  
Virus Type ◽  
Sendai Virus ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Elisa Test ◽  
Serological Response

The method of enzyme-linked immunoadsorbent assay (ELISA) has been applied to the detection of antibodies to reovirus 3, Sendai virus and mouse hepatitis virus (type 1), and the serological response of mice after infection has been followed for 28 days to investigate the earliest appearance of ELISA titres. This has been compared to the appearance of haemagglutination-inhibiting and complement-fixing antibodies. Alkaline phosphatase conjugated antiserum produces the most sensitive and convenient ELISA for the murine viruses examined.

scholarly journals Protective and Pathologic Roles of the Immune Response to Mouse Hepatitis Virus Type 1: Implications for Severe Acute Respiratory Syndrome

2009 ◽  
Vol 83 (18) ◽  
pp. 9258-9272 ◽  
Author(s):  
Aaruni Khanolkar ◽  
Stacey M. Hartwig ◽  
Brayton A. Haag ◽  
David K. Meyerholz ◽  
Lecia L. Epping ◽  
...  
Keyword(s):  
Immune Response ◽  
Severe Acute Respiratory Syndrome ◽  
Virus Type ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Host Immune Response ◽  
Morbidity And Mortality ◽  
Type I ◽  
Lung Pathology

ABSTRACT Intranasal mouse hepatitis virus type 1 (MHV-1) infection of mice induces lung pathology similar to that observed in severe acute respiratory syndrome (SARS) patients. However, the severity of MHV-1-induced pulmonary disease varies among mouse strains, and it has been suggested that differences in the host immune response might account for this variation. It has also been suggested that immunopathology may represent an important clinical feature of SARS. Little is known about the host immune response to MHV-1 and how it might contribute to some of the pathological changes detected in infected mice. In this study we show that an intact type I interferon system and the adaptive immune responses are required for controlling MHV-1 replication and preventing morbidity and mortality in resistant C57BL/6J mice after infection. The NK cell response also helps minimize the severity of illness following MHV-1 infection of C57BL/6J mice. In A/J and C3H/HeJ mice, which are highly susceptible to MHV-1-induced disease, we demonstrate that both CD4 and CD8 T cells contribute to morbidity during primary infection, and memory responses can enhance morbidity and mortality during subsequent reexposure to MHV-1. However, morbidity in A/J and C3H/HeJ mice can be minimized by treating them with immune serum prior to MHV-1 infection. Overall, our findings highlight the role of the host immune response in contributing to the pathogenesis of coronavirus-induced respiratory disease.

Safety and immunogenicity of intranasal murine parainfluenza virus type 1 (Sendai virus) in healthy human adults

Vaccine ◽  
2004 ◽  
Vol 22 (23-24) ◽  
pp. 3182-3186 ◽  
Author(s):  
Karen S Slobod ◽  
Jerry L Shenep ◽  
Jorge Luján-Zilbermann ◽  
Kim Allison ◽  
Brita Brown ◽  
...  
Keyword(s):  
Virus Type ◽  
Sendai Virus ◽  
Parainfluenza Virus ◽  
Healthy Human ◽  
Human Adults

scholarly journals Elevation of Memory Cytotoxic T Lymphocytes, Including Human T Lymphotropic Virus Type 1 Tax-Specific and Hepatitis Virus Type C-Specific Cytotoxic T Lymphocytes, in a Patient with Adult T-Cell Leukemia/Lymphoma and Hepatocellular Carcinoma

2020 ◽  
Vol 13 (2) ◽  
pp. 802-806
Author(s):  
Tatsuro Jo ◽  
Yohei Kaneko ◽  
Takayuki Oishi ◽  
Kaori Matsuzaka ◽  
Haruna Shioya ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Virus Type ◽  
Hepatitis Virus ◽  
Cell Leukemia ◽  
T Lymphotropic Virus ◽  
Adult T Cell Leukemia ◽  
Type C

Herein, we present the case of a patient who suffered from adult T-cell leukemia/lymphoma (ATLL) and hepatocellular carcinoma (HCC) after obtaining a sustained virological response following treatment with a direct-acting antiviral (DAA) at different points in time. The patient went into complete remission (CR) for ATLL. Unfortunately, subsequent relapse of ATLL was observed. This situation was overcome using chemotherapy with pegylated interferon alpha-2b. Human T lymphotropic virus type 1 Tax-specific cytotoxic T lymphocytes (CTLs) were recognized after obtaining second CR, and those CTLs have been maintained for many years. After 4 years from the second CR, chronic hepatitis type C was treated with a DAA, and sustained virological response was attained. However, the occurrence of HCC was detected. Surprisingly, the tumor disappeared spontaneously. Hepatitis virus type C-specific CTLs were also detected in the patient. T-cell receptor (TCR) V beta gene repertoire analyses revealed oligoclonal expansion of effector and memory CTLs. The number of CTLs expressing the TCR V beta 13.1 has increased over the years since HCC occurrence. The activation and maintenance of anticancer cellular immunity may have allowed the patient to obtain long-term survival and overcome two lethal neoplasms.

Prevalence of indigenous viruses in laboratory animal colonies in the United Kingdom 1978-1979

1980 ◽  
Vol 14 (4) ◽  
pp. 309-311 ◽  
Author(s):  
J. Gannon ◽  
P. Carthew
Keyword(s):  
United Kingdom ◽  
Laboratory Animal ◽  
Sendai Virus ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Serological Survey ◽  
The United Kingdom ◽  
Corona Viruses

Compared with the results of the previous serological survey of 1976-1977, it can be seen that mouse hepatitis virus is still prevalent in the mouse colonies and that corona viruses of rats are also common. The prevalence of Sendai virus has increased considerably. However, the prevalence of Reo 3 virus appeared to have decreased, although this may be the result of the different test used.

Immunopathology of mouse hepatitis virus type 3 infection

1982 ◽  
Vol 23 (2) ◽  
pp. 539-547 ◽  
Author(s):  
D. Leray ◽  
C. Dupuy ◽  
J.M. Dupuy
Keyword(s):  
Virus Type ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Type 3
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