Detection of human cytomegalovirus retinitis and monitoring of ganciclovir treatment using conjunctival swab with polymerase chain reaction in AIDS patients

This report studies the accuracy of conjunctival swab polymerase chain reaction (CS-PCR) for the diagnosis of human cytomegalovirus retinitis (HCMV) in AIDS patients. PCR and virus culture were used for the detection of HCMV in conjunctival swab, serum, and urine specimens from 38 AIDS patients between April 1996 and April 1998. The clinical utility of the identification of HCMV retinitis by these 6 different methods was demonstrated by their prediction power to estimate AIDS patients at risk of contracting HCMV retinitis. The sensitivity, specificity, positive predictive value, and negative predictive value of CS-PCR for the detection of HCMV retinitis were 91.5%, 80.9%, 60.8%, and 92.7%, respectively; for serum PCR were 74.3%, 81.7%, 57.2%, and 90.3%; for urine PCR were 100%, 17.3%, 20.4%, and 100%; for conjunctival swab culture were 22.7%, 100%, 100%, and 86%; for serum culture were 27.3%, 98.1%, 75%, and 86.4%; and for urine culture were 90.9%, 44.2%, 25.6%, and 95.8%.

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Using Conjunctival Swab with Polymerase Chain Reaction to Aid Diagnosis of Cytomegalovirus Retinitis in AIDS Patients

Ophthalmologica ◽

10.1159/000027481 ◽

2000 ◽

Vol 214 (2) ◽

pp. 126-130 ◽

Cited By ~ 2

Author(s):

Jorn-Hon Liu ◽

Wen-Ming Hsu ◽

Wing-Wai Wong ◽

Jaang-Jiun Wang ◽

Wu-Tse Liu ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Cytomegalovirus Retinitis ◽

Chain Reaction ◽

Aids Patients ◽

Conjunctival Swab ◽

Polymerase Chain

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Early virus isolation, early structural antigen detection and DNA amplification by the polymerase chain reaction in polymorphonuclear leuckocytes from AIDS patients with human cytomegalovirus viraemia

Molecular and Cellular Probes ◽

10.1016/s0890-8508(06)80008-3 ◽

1991 ◽

Vol 5 (5) ◽

pp. 365-374 ◽

Cited By ~ 22

Author(s):

Giuseppe Gerna ◽

Donato Zipeto ◽

Maurizio Parea ◽

Elena Percivalle ◽

Maurizio Zavattoni ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Human Cytomegalovirus ◽

Virus Isolation ◽

Dna Amplification ◽

Antigen Detection ◽

Chain Reaction ◽

Aids Patients ◽

Polymerase Chain ◽

Structural Antigen

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Serum Polymerase Chain Reaction for Cytomegalovirus DNA for Monitoring Ganciclovir Treatment in AIDS Patients

Scandinavian Journal of Infectious Diseases ◽

10.3109/00365549609037917 ◽

1996 ◽

Vol 28 (4) ◽

pp. 347-351 ◽

Cited By ~ 4

Author(s):

Roberta Marenzi ◽

Paola Cinque ◽

Daniela Ceresa ◽

Sara Racca ◽

Flavia Lillo ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Chain Reaction ◽

Aids Patients ◽

Ganciclovir Treatment ◽

Polymerase Chain

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Polymerase chain reaction analysis of aqueous humor specimens in the diagnosis of cytomegalovirus retinitis in AIDS patients

European Journal of Ophthalmology ◽

10.1177/1120672119841777 ◽

2019 ◽

Vol 30 (4) ◽

pp. 738-742

Author(s):

Feifei Mao ◽

Huiyu Sun ◽

Dan Li ◽

Shengnan Wang ◽

Dan Lu

Keyword(s):

Polymerase Chain Reaction ◽

Aqueous Humor ◽

Polymerase Chain Reaction Analysis ◽

Cytomegalovirus Retinitis ◽

Chain Reaction ◽

Aids Patients ◽

Blood Samples ◽

Diagnostic Categories ◽

The Mean ◽

Polymerase Chain

Purpose: To determine the value of the polymerase chain reaction analysis of aqueous humor specimens as a tool to diagnose cytomegalovirus retinitis in AIDS patients. Methods: In all, 63 AIDS patients were evaluated in this study. They were sorted into two diagnostic categories: eyes with active cytomegalovirus retinitis and eyes without active cytomegalovirus retinitis. The aqueous humor and blood samples were collected and analyzed by polymerase chain reaction. Results: A total of 49 patients had active cytomegalovirus retinitis (77.8%) and 14 patients had inactive cytomegalovirus retinitis or normal fundus (22.2%). The mean average of patients was 39 years (range: 22–59). The majority of patients were male (90.5%). Cytomegalovirus DNA was detected in 46 and 7 of 49 aqueous and blood samples, respectively, from AIDS patients with active cytomegalovirus retinitis. We did not detect cytomegalovirus DNA in any of the eyes without active cytomegalovirus retinitis. The sensitivity of polymerase chain reaction in the detection of cytomegalovirus in aqueous humor and blood samples was 93.5% and 14.3%, respectively. Conclusions: The polymerase chain reaction analysis is a safe, highly specific, and sensitive method to diagnose cytomegalovirus retinitis.

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