scholarly journals Close genetic relationship between cultivated and natural populations of common buckwheat in the Sanjiang area is not due to recent gene flow between them—An analysis using microsatellite markers

2007 ◽  
Vol 82 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Takehiko Konishi ◽  
Ohmi Ohnishi
Keyword(s):  
Gene Flow ◽  
Microsatellite Markers ◽  
Genetic Relationship ◽  
Natural Populations ◽  
Common Buckwheat ◽  
Recent Gene Flow ◽  
Close Genetic Relationship

scholarly journals SSR Markers for Analysing South American Nothofagus Species

2004 ◽  
Vol 53 (1-6) ◽  
pp. 240-243 ◽  
Author(s):  
M. M. Azpilicueta ◽  
H. Caron ◽  
C. Bodénès ◽  
L. A. Gallo
Keyword(s):  
Gene Flow ◽  
Genetic Mapping ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Natural Populations ◽  
South American ◽  
Sample Sizes ◽  
Polymorphic Ssrs ◽  
The Cross ◽  
Qualitative Confirmation

Summary11 newly discovered microsatellites were used to identify SSR markers for characterising South American Nothofagus species. This was carried out in six species. The sample sizes used were between four and six individuals per species. The cross-genera transferability of 34 Quercus SSRs was also essayed. Out of the 11 new microsatellite markers, three proved to be polymorphic (NnBIO 11, NgBIO 13 and NgBIO 14). The qualitative confirmation of the inheritance of these markers could also be verified. Polymorphism was also observed in five of the cross-genera transferred SSRs (QrBIO7, quru-GA-0A01, quru-GA-0C11, quru-GA-0I01, quru-GA-0M07). The number of alleles per locus found range between 1 and 6 per species. The eight polymorphic SSRs identified in this study will constitute a valuable tool in the gene flow studies that are currently being carried out in natural populations of South American Nothofagus species. The confirmation of crossspecies and cross-genera transferability opens the way for the use of SSRs as bridge markers in genetic mapping.

Gene flow in three broiler chicken populations in Abeokuta using jackknife procedure

2021 ◽  
Vol 43 (2) ◽  
pp. 1-12
Author(s):  
O. Olowofeso ◽  
M. A. Adeleke ◽  
M. A Azeez ◽  
A. O. Adesegun
Keyword(s):  
Gene Flow ◽  
Microsatellite Markers ◽  
Broiler Chicken ◽  
Natural Populations ◽  
Total Sample ◽  
Chicken Population ◽  
Polymerase Chain ◽  
Polymorphic Microsatellite ◽  
Estimate Gene Flow ◽  
Jackknife Procedure

Jackknife procedure (JP) is a less biased and fascinating method of obtaining gene flow among populations. The purpose of this study was to use JP to eliminate bias associated with indirect estimators of gene flow. With microsatellite markers, it has been possible to estimate gene flow (Nmo ) in natural populations. To quantify Nmo in chicken populations, we used five polymorphic microsatellite markers with 115 genomic deoxyribonucleic acid (DNA) obtained from (dihybrid (DH = 37), trihybrid (TH = 32) and Anak White (AW = 46) broiler chicken populations, respectively. Through polymerase chain reaction, we amplified DNA from the broiler chicken populations, subjected amplicons to electrophoresis, fragment sizes determined and analysed across populations considering selected markers through which standardized genetic variance among sub-populations in total sample depicted as (F)ST was obtained per marker for chicken population pairs. Its average across markers/population pairs was used to infer Nmo  in the chicken population pairs. We used JP which is a mathematical approach that utilizes mean FST across markers to obtain Nmo in the chicken population pairs. Gene flow based on JP in chicken population pairs designated as (Nm)JP were 5.4267 (DH vs. TH), 7.0127 (TH vs. AW) and 11.7405 (DH vs. AW) and among chicken populations, (Nm)JP was 7.1969. Considering these estimates, we concluded that there was JP gene flow among the three broiler chicken populations examined in Abeokuta, Nigeria.

scholarly journals Gene flow in three broiler chicken populations in Abeokuta using jackknife procedure

2021 ◽  
Vol 43 (1) ◽  
pp. 1-12
Author(s):  
O. Olowofeso ◽  
M. A. Adeleke ◽  
M. A. Azeez ◽  
A. O. Adesegun
Keyword(s):  
Gene Flow ◽  
Microsatellite Markers ◽  
Broiler Chicken ◽  
Natural Populations ◽  
Total Sample ◽  
Chicken Population ◽  
Polymerase Chain ◽  
Estimate Gene Flow ◽  
Jackknife Procedure ◽  
Was Gene

Jackknife procedure (JP) is a less biased and fascinating method of obtaining gene slow among populations. The purpose of this study was to use JP to eliminate bias associated with indirect estimators of gene slow, with microsatellite markers, it has been possible to estimate gene flow (Nm) in natural populations. To quantify Nm, in chicken populations, we used five polymorphic microsatellite markers with 115 genomic deoxyribonucleic acid (DNA) obtained from (dihybrid (DH = 37), trihybrid (TH = 32) and Anak White (AW = 46) broiler chicken populations, respectively. Through polymerase chain reaction, we amplified DNA from the broiler chicken populations, subjected amplicons to electrophoresis, fragment sizes determined and analysed across populations considering selected markers through which standardized genetic variance among sub-populations in total sample depicted as (FST) was obtained per marker for chicken population pairs. Its average across markers/population pairs was used to infer Nm, in the chicken population pairs. We used JP which is a mathematical approach that utilizes mean FST, across markers to obtain Nm in the chicken population pairs. Gene flow based on JP in chicken population pairs designated as (Nm)JP were 5.4267 (DH vs. TH), 7.0127 (TH vs. AW) and 11.7405 (DH vs. AW) and among chicken populations, (Nm)JP was 7.1969. Considering these estimates, we concluded that there was gene flow among the three broiler chicken populations examined in Abeokuta, Nigeria.

scholarly journals Measuring recent effective gene flow among large populations in Pinus sylvestris: Local pollen shedding does not preclude substantial long-distance pollen immigration

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255776
Author(s):  
Azucena Jiménez-Ramírez ◽  
Delphine Grivet ◽  
Juan José Robledo-Arnuncio
Keyword(s):  
Gene Flow ◽  
Pinus Sylvestris ◽  
Molecular Genetic ◽  
Natural Populations ◽  
Common Garden ◽  
Long Distance ◽  
Chloroplast Microsatellite ◽  
Flow Rates ◽  
Recent Gene Flow ◽  
Large Tree

The estimation of recent gene flow rates among vast and often weakly genetically differentiated tree populations remains a great challenge. Yet, empirical information would help understanding the interaction between gene flow and local adaptation in present-day non-equilibrium forests. We investigate here recent gene flow rates between two large native Scots pine (Pinus sylvestris L.) populations in central Iberian Peninsula (Spain), which grow on contrasting edaphic conditions six kilometers apart from each other and show substantial quantitative trait divergence in common garden experiments. Using a sample of 1,200 adult and offspring chloroplast-microsatellite haplotypes and a Bayesian inference model, we estimated substantial male gametic gene flow rates (8 and 21%) between the two natural populations, and even greater estimated immigration rates (42 and 64%) from nearby plantations into the two natural populations. Our results suggest that local pollen shedding within large tree populations does not preclude long-distance pollen immigration from large external sources, supporting the role of gene flow as a homogenizing evolutionary force contributing to low molecular genetic differentiation among populations of widely distributed wind-pollinated species. Our results also indicate the high potential for reproductive connectivity in large fragmented populations of wind-pollinated trees, and draw attention to a potential scenario of adaptive genetic divergence in quantitative traits under high gene flow.

Genetic characterization of novel polymorphic microsatellite markers for Epilobium nankotaizanense (Onagraceae), an endemic and threatened herb in Taiwan

2021 ◽  
pp. 1-4
Author(s):  
Yu-Wei Tseng ◽  
Chi-Chun Huang ◽  
Chih-Chiang Wang ◽  
Chiuan-Yu Li ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Natural Populations ◽  
Conservation Strategy ◽  
Sequencing Data ◽  
Germplasm Collections ◽  
The Family ◽  
Conservation Genetic ◽  
Population Sizes ◽  
Polymorphic Microsatellite

Abstract Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.

scholarly journals Contrasting Historical and Recent Gene Flow among African Buffalo Herds in the Caprivi Strip of Namibia

2013 ◽  
Vol 104 (2) ◽  
pp. 172-181 ◽  
Author(s):  
Clinton W. Epps ◽  
Jessica A. Castillo ◽  
Anne Schmidt-Küntzel ◽  
Pierre du Preez ◽  
Greg Stuart-Hill ◽  
...  
Keyword(s):  
Gene Flow ◽  
African Buffalo ◽  
Recent Gene Flow ◽  
Caprivi Strip

scholarly journals Advanced genebank management of genetic resources of European wild apple, Malus sylvestris, using genome-wide SNP array data

2021 ◽  
Vol 17 (4) ◽  
Author(s):  
Joukje Buiteveld ◽  
Herma JJ Koehorst-van Putten ◽  
Linda Kodde ◽  
Ivo Laros ◽  
Giorgio Tumino ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Genetic Relationship ◽  
Snp Array ◽  
Seed Orchard ◽  
Effective Population ◽  
Malus Sylvestris ◽  
Genebank Management ◽  
Genetic Resources Management ◽  
Genebank Collection ◽  
Close Genetic Relationship

AbstractThe Netherlands’ field genebank collection of European wild apple (Malus sylvestris), consisting of 115 accessions, was studied in order to determine whether duplicates and mistakes had been introduced, and to develop a strategy to optimize the planting design of the collection as a seed orchard. We used the apple 20K Infinium single nucleotide polymorphism (SNP) array, developed in M. domestica, for the first time for genotyping in M. sylvestris. We could readily detect the clonal copies and unexpected duplicates. Thirty-two M. sylvestris accessions (29%) showed a close genetic relationship (parent-child, full-sib, or half-sib) to another accession, which reflects the small effective population size of the in situ populations. Traces of introgression from M. domestica were only found in 7 individuals. This indicates that pollination preferentially took place among the M. sylvestris trees. We conclude that the collection can be considered as mainly pure M. sylvestris accessions. The results imply that it should be managed as one unit when used for seed production. A bias in allele frequencies in the seeds may be prevented by not harvesting all accessions with a close genetic relationship to the others in the seed orchard. We discuss the value of using the SNP array to elaborate the M. sylvestris genetic resources more in depth, including for phasing the markers in a subset of the accessions, as a first step towards genetic resources management at the level of haplotypes.

To what extent do microsatellite markers reflect genome-wide genetic diversity in natural populations?

2008 ◽  
Vol 17 (17) ◽  
pp. 3808-3817 ◽  
Author(s):  
ÜLO VÄLI ◽  
ANNIKA EINARSSON ◽  
LISETTE WAITS ◽  
HANS ELLEGREN
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Natural Populations ◽  
Genome Wide

scholarly journals Genetic structure and gene flow of Eugenia dysenterica natural populations

2005 ◽  
Vol 40 (10) ◽  
pp. 975-980 ◽  
Author(s):  
Maria Imaculada Zucchi ◽  
José Baldin Pinheiro ◽  
Lázaro José Chaves ◽  
Alexandre Siqueira Guedes Coelho ◽  
Mansuêmia Alves Couto ◽  
...  
Keyword(s):  
Gene Flow ◽  
Genetic Variability ◽  
Pearson Correlation ◽  
Natural Populations ◽  
Similarity Index ◽  
Genetic Distances ◽  
Strong Positive Correlation ◽  
Randomly Amplified Polymorphic Dna ◽  
Jaccard Similarity ◽  
Eugenia Dysenterica

This study was carried out to assess the genetic variability of ten "cagaita" tree (Eugenia dysenterica) populations in Southeastern Goiás. Fifty-four randomly amplified polymorphic DNA (RAPD) loci were used to characterize the population genetic variability, using the analysis of molecular variance (AMOVA). A phiST value of 0.2703 was obtained, showing that 27.03% and 72.97% of the genetic variability is present among and within populations, respectively. The Pearson correlation coefficient (r) among the genetic distances matrix (1 - Jaccard similarity index) and the geographic distances were estimated, and a strong positive correlation was detected. Results suggest that these populations are differentiating through a stochastic process, with restricted and geographic distribution dependent gene flow.

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