scholarly journals LOCALIZATION OF ANTI-ALLERGIC AGENT IN RAT MAST CELLS DEMONSTRATED BY LIGHT AND ELECTRON MICROSCOPIC RADIOAUTOGRAPHY

1986 ◽  
Vol 19 (5) ◽  
pp. 669-683 ◽  
Author(s):  
TETSUJI NAGATA ◽  
TOSHIAKI NISHIGAKI ◽  
YASUNORI MOMOSE
Keyword(s):  
Mast Cells ◽  
Electron Microscopic ◽  
Electron Microscopic Radioautography ◽  
Rat Mast Cells

Immuno-electron microscopic localization of fibronectin on rat mast cells

1982 ◽  
Vol 38 (4) ◽  
pp. 495-496
Author(s):  
J. Sasaki ◽  
M. Imanaka ◽  
S. Watanabe ◽  
N. Otsuka ◽  
K. Sugiyama
Keyword(s):  
Mast Cells ◽  
Electron Microscopic ◽  
Microscopic Localization ◽  
Rat Mast Cells ◽  
Electron Microscopic Localization

scholarly journals Electron Microscopic Studies on the Inhibition of Degranulation of Rat Mast Cells by a Novel Anti-Allergic Agent, PTPC

1994 ◽  
Vol 66 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Tetsuo Ohashi ◽  
Akihiko Watanabe ◽  
Toshiaki Nishigaki ◽  
Hiroshi Azuma
Keyword(s):  
Mast Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Rat Mast Cells

Electron microscopic demonstration of metals in rat mast cells

1980 ◽  
Vol 66 (3) ◽  
pp. 293-300 ◽  
Author(s):  
G. Danscher ◽  
J. Obel ◽  
O. Thorlacius-Ussing
Keyword(s):  
Mast Cells ◽  
Electron Microscopic ◽  
Microscopic Demonstration ◽  
Electron Microscopic Demonstration ◽  
Rat Mast Cells

A simplified method of emulsion coating and development for quantitative electron microscopic radioautography

1978 ◽  
Vol 36 (2) ◽  
pp. 64-65
Author(s):  
K. Yoshida ◽  
F. Murata ◽  
S. Ohno ◽  
T. Nagata
Keyword(s):  
Mass Production ◽  
Identical Condition ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Simplified Method ◽  
Complicated Process ◽  
Critical Problems ◽  
Electron Microscopic Radioautography ◽  
Quantitative Radioautography

IntroductionSeveral methods of mounting emulsion for radioautography at the electron microscopic level have been reported. From the viewpoint of quantitative radioautography, however, there are many critical problems in the procedure to produce radioautographs. For example, it is necessary to apply and develop emulsions in several experimental groups under an identical condition. Moreover, it is necessary to treat a lot of grids at the same time in the dark room for statistical analysis. Since the complicated process and technical difficulties in these procedures are inadequate to conduct a quantitative analysis of many radioautographs at once, many factors may bring about unexpected results. In order to improve these complicated procedures, a simplified dropping method for mass production of radioautographs under an identical condition was previously reported. However, this procedure was not completely satisfactory from the viewpoint of emulsion homogeneity. This paper reports another improved procedure employing wire loops.

Platelet Incorporation of Labelled Adenosine and Adenosine Diphosphate

1969 ◽  
Vol 22 (02) ◽  
pp. 304-315 ◽  
Author(s):  
E. W Salzman ◽  
T. P Ashford ◽  
D. A Chambers ◽  
Lena L. Neri
Keyword(s):  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
Platelet Inhibition ◽  
Electron Microscopic ◽  
Platelet Binding ◽  
Minor Degree ◽  
A Minor ◽  
Plasma Marker ◽  
Electron Microscopic Radioautography ◽  
Simultaneous Assessment

SummaryAfter incubation of platelet-rich plasma with labelled adenosine or ADP, platelet incorporation of radioactivity was assessed. Platelets were rapidly separated for counting by filtration through cellulose acetate Millipore. Inulin-H3 served as a plasma marker, and triple isotope techniques permitted simultaneous assessment of the behavior of the adenine and phosphate moieties of ADP without washing of platelets. In other experiments, electron microscopic radioautography was employed to trace the label after platelet incorporation.The results were consistent with previous reports that ADP is dephosphorylated in plasma and is incorporated by platelets only as a dephosphorylated residue, probably adenosine. The label crossed the platelet membrane and entered the platelet, where it was distributed in platelet granules and the agranular cell sap. Concentration within granules occurred to a minor degree.The results support the hypothesis that platelet aggregation by ADP occurs without a persistent bond of ADP to the platelet. Inhibition of aggregation by adenosine probably depends on a metabolic or transport process rather than on competition between adenosine and ADP for platelet binding sites.

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