A Comparative Cytochemical Study of the Dufour Gland in the Eusocial Bee Apis mellifera Linné, 1758 and Melipona bicolor Lepeletier, 1836

Um levantamento dos estudos melissopalinológicos realizados no Rio Grande do Sul é aqui apresentado. O intuito é contribuir para o conhecimento nessa área e identificar as lacunas de informações para que sejam propostos estudos que complementem as informações sobre a flora apícola desse estado, um dos principais produtores de mel do Brasil. Os estudos encontrados na literatura foram realizados com mel de Apis mellifera e dos meliponíneos Tetragonisca angustula, Scaptotrigona bipunctata, Melipona obscurior, Melipona bicolor, Melipona quadrifasciata, Plebeia emerina e Plebeia remota. Os méis são classificados como heteroflorais, com predominância de Schinus terebenthifolia, Ilex sp., Syagrus romanzoffiana, Asteraceae, Clethra scabra, Sebastiania sp., Eucalyptus sp., Mimosa sp., Eugenia sp. e Hovenia dulcis. O levantamento demonstra que o número de análises realizadas até o momento não corresponde à quantidade de produção de mel do Rio Grande Sul e que são necessários mais estudos da flora apícola em outras áreas do estado do que aquelas aqui avaliadas para a caracterização botânica e melissopalinológica. Espera-se sensibilizar os órgãos de fomento de pesquisa e a cadeia produtiva para conhecer e promover estratégias de manejo da flora apícola a fim de valorizar a apicultura no Rio Grande do Sul.

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Occurrence, morphology and ultrastructure of the Dufour gland in Melipona bicolor Lepeletier (Hymenoptera, Meliponini)

Revista Brasileira de Entomologia ◽

10.1590/s0085-56262004000100002 ◽

2004 ◽

Vol 48 (1) ◽

pp. 9-19 ◽

Cited By ~ 5

Author(s):

Fábio Camargo Abdalla ◽

Carminda da Cruz-Landim

Keyword(s):

Dufour Gland ◽

Melipona Bicolor

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Chemical composition of the dufour gland secretion in queens of Melipona bicolor (Hymenoptera, Meliponini)

Journal of the Brazilian Chemical Society ◽

10.1590/s0103-50532004000500002 ◽

2004 ◽

Vol 15 (5) ◽

pp. 621-625 ◽

Cited By ~ 12

Author(s):

Fábio C. Abdalla ◽

Graeme R. Jones ◽

David Morgan ◽

Carminda da Cruz-Landim

Keyword(s):

Chemical Composition ◽

Dufour Gland ◽

Gland Secretion ◽

Melipona Bicolor

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Electron Cytochemical Study of Carbon Dioxide Laser Effect on Rhesus Monkey Cornea

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010005113x ◽

1974 ◽

Vol 32 ◽

pp. 224-225

Author(s):

K. C. Tsou ◽

J. Morris ◽

P. Shawaluk ◽

B. Stuck ◽

E. Beatrice

Keyword(s):

Carbon Dioxide ◽

Electron Microscope ◽

Rhesus Monkey ◽

Carbon Dioxide Laser ◽

Cytochemical Study ◽

Laser Effect

While much is known regarding the effect of lasers on the retina, little study has been done on the effect of lasers on cornea, because of the limitation of the size of the material. Using a combination of electron microscope and several newly developed cytochemical methods, the effect of laser can now be studied on eye for the purpose of correlating functional and morphological damage. The present paper illustrates such study with CO2 laser on Rhesus monkey.

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The Fine Structure of Glycocalyx in Human Spermatozoa. A High Resolution Cytochemical Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073647 ◽

1973 ◽

Vol 31 ◽

pp. 640-641

Author(s):

P. Hernández-Jáuregui ◽

A. Sosa ◽

A. González Angulo

Keyword(s):

Negative Charge ◽

Iron Hydroxide ◽

Human Spermatozoa ◽

Surface Coat ◽

Cell Surfaces ◽

Colloidal Iron ◽

Cytochemical Study ◽

Specific Permeability ◽

Extracellular Glycoprotein ◽

Mammalian Spermatozoa

Glycocalyx is the name given by Bennett to the extracellular glycoprotein coat present in some cell surfaces. It appears to play an important role in cell properties such as antigenicity, cell adhesivity, specific permeability, and ATP ase activity. In the sperm this coat can be directly related to such important phenomena as capacitation and fertilization. The presence of glycocalyx in invertebrate spermatozoa has already been demonstrated. Recently Yanagimachi et al. has determined the negative charges on sperm surfaces of mammalian spermatozoa including man, using colloidal iron hydroxide. No mention was made however of the outer surface coat as composed of substances other than those confering a negative charge. The purpose of this work was therefore to determine the presence of a glycocalyx in human spermatozoa using alcian blue and lanthanum staining.

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Effect of ADH on Rat Renal Papilla, an Ultrastructural and Cytochemical Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072496 ◽

1973 ◽

Vol 31 ◽

pp. 410-411

Author(s):

C. N. Sun ◽

H. J. White ◽

E. J. Towbin

Keyword(s):

Electron Microscopy ◽

Diabetes Insipidus ◽

Renal Papilla ◽

Milk Diet ◽

Intercellular Spaces ◽

Cytochemical Study ◽

Collecting Tubule ◽

Concentrate Urine ◽

Staining Techniques ◽

Collecting Tubules

Diabetes insipidus and compulsive water drinking are representative of two categories of antidiuretic hormone (ADH) lack. We studied a strain of rats with congenital diabetes insipidus homozygote (DI) and normal rats on an isocaloric fortified dilute milk diet. In both cases, the collecting tubules could not concentrate urine. Special staining techniques, Alcian Blue-PAS for light microscopy and lanthanum nitrate for electron microscopy were used to demonstrate the changes in interstitial mucopolysaccharides (MPS). The lanthanum staining was done according to the method of Khan and Overton.Electron microscopy shows cytoplasmic lesions, vacules, swelling and degenerating mitochondria and intercellular spaces (IS) in the collecting tubule cells in DI and rats on milk diet.

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In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083035 ◽

1978 ◽

Vol 36 (2) ◽

pp. 434-435

Author(s):

D. Reis ◽

B. Vian ◽

J. C. Roland

Keyword(s):

Cell Wall ◽

Self Assembly ◽

Plant Cell Wall ◽

Higher Plants ◽

Three Dimensional ◽

Cytochemical Study ◽

Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

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A High Resolution Cytochemical Study of Nuclear ATPase in Human Spermatozoa

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100116590 ◽

1975 ◽

Vol 33 ◽

pp. 594-595

Author(s):

A. Sosa ◽

L. Calzada

Keyword(s):

High Resolution ◽

Atpase Activity ◽

Nuclear Membrane ◽

Human Sperm ◽

Sperm Nucleus ◽

Human Spermatozoa ◽

Cytochemical Study ◽

Membrane Bound ◽

Sperm Nuclei ◽

Interchromatin Space

The dependence of nuclear metabolism on the function of the nuclear membrane is not well understood. Whether or not the function of the nuclear membrane is partial or totally responsible of the repressed template activity of human sperm nucleus has not at present been elucidated. One of the membrane-bound enzymatic activities which is concerned with the mechanisms whereby substances are thought to cross cell membranes is adenosintriphosphatase (ATPase). This prompted its characterization and distribution by high resolution photogrammetry on isolated human sperm nuclei. Isolated human spermatozoa nuclei were obtained as previously described. ATPase activity was demonstrated by the method of Wachstein and Meisel modified by Marchesi and Palade. ATPase activity was identified as dense and irregularly distributed granules confined to the internal leaflet of the nuclear membrane. Within the nucleus the appearance of the reaction product occurs as homogenous and dense precipitates in the interchromatin space.

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