Study on Female Gametophyte Development of Primulina tabacum

Background Sugarcane (Saccharum spontaneum L.), the major sugar and biofuel feedstock crop, is cultivated mainly by vegetative propagation worldwide due to the infertility of female reproductive organs resulting in the reduction of quality and output of sugar. Deciphering the gene expression profile during ovule development will improve our understanding of the complications underlying sexual reproduction in sugarcane. Optimal reference genes are essential for elucidating the expression pattern of a given gene by quantitative real-time PCR (qRT-PCR). Method In this study, based on transcriptome data obtained from sugarcane ovule, eighteen candidate reference genes were identified, cloned, and their expression levels were evaluated across five developmental stages ovule (AC, MMC, Meiosis, Mitosis, and Mature). Results Our results indicated that FAB2 and MOR1 were the most stably expressed genes during sugarcane female gametophyte development. Moreover, two genes, cell cycle-related genes REC8 and CDK, were selected, and their feasibility was validated. This study provides important insights into the female gametophyte development of sugarcane and reports novel reference genes for gene expression research on sugarcane sexual reproduction.

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Female gametophyte development in Eleocharis sellowiana (CYPERACEAE): a species with atypical nuclear staining and polarity of the megaspore mother cell

Plant Systematics and Evolution ◽

10.1007/s00606-014-1145-2 ◽

2014 ◽

Vol 301 (4) ◽

pp. 1287-1292 ◽

Cited By ~ 3

Author(s):

Danilo M. Rocha ◽

Célia G. T. J. Andrade ◽

André L. L. Vanzela

Keyword(s):

Mother Cell ◽

Megaspore Mother Cell ◽

Female Gametophyte ◽

Nuclear Staining ◽

Gametophyte Development ◽

Female Gametophyte Development

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Seed production and gametophyte formation in Agave tequilana and Agave americana

Botany ◽

10.1139/b08-099 ◽

2008 ◽

Vol 86 (11) ◽

pp. 1343-1353 ◽

Cited By ~ 25

Author(s):

Rocio E. Escobar-Guzmán ◽

Flor Zamudio Hernández ◽

Katia Gil Vega ◽

June Simpson

Keyword(s):

Seed Production ◽

Female Gametophyte ◽

Male Gametophyte ◽

Raw Material ◽

Low Fertility ◽

Agave Tequilana ◽

Genetic Incompatibility ◽

Gametophyte Development ◽

Agave Americana ◽

Female Gametophyte Development

Agave tequilana Weber var. azul is the raw material used in the production of tequila. This species has a life cycle of approximately 6–8 years; however, owing to the practice of removing the inflorescence to conserve accumulated sugar reserves, the main form of reproduction is asexual. Little attention has, therefore, been paid to the process of flowering and the factors leading to low levels of germination and seedling viability have not been investigated in detail. The objective of this study was to document gametophyte development, seed production, and germination in A. tequilana under different pollination treatments and in an interspecies cross with Agave americana L. Seed production and germination efficiency was low for both A. tequilana and A. americana under the different pollination treatments, although interspecies crosses did produce some viable seeds. Development of the male gametophyte in both species is of the successive type, producing pollen grains with dicolpate morphology. Female gametophyte development is of the Polygonum monosporic type. The results obtained suggest that genetic incompatibility, inbreeding effects, factors affecting pollen development and germination, or errors in female gametophyte development may contribute to the low fertility observed for A. tequilana and A. americana.

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Female gametophyte development

Journal of Plant Biology ◽

10.1007/s12374-013-0131-5 ◽

2013 ◽

Vol 56 (6) ◽

pp. 345-356 ◽

Cited By ~ 5

Author(s):

Ai Guo ◽

Cai Xia Zheng

Keyword(s):

Female Gametophyte ◽

Gametophyte Development ◽

Female Gametophyte Development

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Ovule and Female Gametophyte Development in Fertile and Sterile Safflower Plants (Carthamus tinctorius L.)

Australian Journal of Botany ◽

10.1071/bt9890519 ◽

1989 ◽

Vol 37 (6) ◽

pp. 519 ◽

Cited By ~ 3

Author(s):

J Carapetian ◽

EA Rupert

Keyword(s):

Cell Differentiation ◽

Female Gametophyte ◽

Embryo Sac ◽

Carthamus Tinctorius ◽

Gametophyte Development ◽

Male Gametogenesis ◽

Delayed Initiation ◽

Rapid Elongation ◽

Functional Megaspore ◽

Female Gametophyte Development

Development of safflower ovules and female gametophytes was compared in fertile and genetically sterile F2 and backcross segregants from the cross between 'US-10' and '57-147' genotypes. Fertile plants formed normal anatropous ovules with eight-nucleate embryo sacs, typical of the angiosperms. One week before anthesis, the eight-nucleate embryo sac is well developed and undergoes rapid elongation and expansion during the 24 h prior to anthesis, accompanied by a doubling in length of the florets. Sterile plants also formed normal ovules, but apparently with a delayed initiation of meiosis which was subsequently arrested at Metaphase I. Embryo sacs did not form in sterile florets except for rare observations of uninucleate embryo sacs which began to degenerate before anthesis. The integumentary tapetum which normally developed upon completion of meiosis in fertile plants, was well developed during Prophase I of megasporogenesis in sterile plants. This observation suggests that cell differentiation and development of this nutritive jacket is basically controlled by the age of the ovules rather than initiated by appearance of the functional megaspore. Failure of both female and male gametogenesis seems to result from interaction of three independently segregating genes.

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