scholarly journals Characterization of Panton–Valentine leukocidin-positive Staphylococcus aureus from skin and soft tissue infections and wounds in Nigeria: a cross-sectional study

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1155 ◽  
Author(s):  
Olayemi O. Ayepola ◽  
Nurudeen A. Olasupo ◽  
Louis O. Egwari ◽  
Frieder Schaumburg
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Western Blotting ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Soft Tissue Infections ◽  
Accessory Gene ◽  
Cross Sectional ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Background: Staphylococcus aureus is a significant pathogen implicated in numerous nosocomial and community-acquired infections. The Panton–Valentine leukocidin (PVL) can be associated with severe necrotizing diseases such as pneumonia, skin and soft tissue infection (SSTI).  Methods: In total, 96 S. aureus isolates were obtained from patients presenting with wounds (n=48) and soft tissue infections (SSTIs, n=48). These were characterized based on their antimicrobial susceptibility profile, the possession of virulence genes (e.g. capsular type, PVL), accessory gene regulator (agr) type, and the staphylococcal protein A (spa) type. The production of the PVL protein was assessed by western blotting. Results: All isolates were susceptible to methicillin. The resistance was highest to penicillin (97.9%), followed by trimethoprim/sulfamethoxazole (85.4%) and tetracycline (10.4%). The PVL gene was found in 83.3% of isolates from SSTIs and in 79.2% of isolates from wound. Of these, 53 (68%) produced PVL as assessed by western blotting. The most prevalent spa type was the t084 (78.1%, n=75) and, majority of the isolates carried agr2 (82.3%, n=79). Conclusions: Prevalence of antibiotic resistant PVL-positive methicillin susceptible S. aureus strains has severe implications on PVL mediated infections.

scholarly journals Characterisation of community-acquired Staphylococcus aureus causing skin and soft tissue infections in a children's hospital in Shanghai, China

2019 ◽  
Vol 147 ◽  
Author(s):  
Xing Wang ◽  
Yanyun Shen ◽  
Weichun Huang ◽  
Yun Zhou
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Virulence Genes ◽  
Protein A ◽  
Molecular Characteristics ◽  
Staphylococcal Protein A ◽  
Soft Tissue Infections ◽  
Mrsa Strains ◽  
Panton Valentine Leukocidin ◽  
Common Sequence

Abstract Community-acquired Staphylococcus aureus is a major pathogen responsible for skin and soft tissue infections (SSTIs). This study aimed to investigate the prevalence and molecular characteristics of community-acquired S. aureus isolates recovered from paediatric patients with SSTIs in Shanghai, China. Between January 2015 and January 2018, 91 community-acquired S. aureus isolates were characterised by antibiotic susceptibility, multilocus sequence typing (ST), staphylococcal protein A gene (spa) type and virulence genes. Methicillin-resistant S. aureus (MRSA) strains were also characterised by staphylococcal cassette chromosome mec (SCCmec) type. Forty-one (45.1%) S. aureus isolates were MRSA. ST59 (33.0%, 30/91) was the most common sequence type, and t437 (18.7%, 17/91) was the most common spa type. SCCmec IV and V accounted for 61.0% and 34.1% of all MRSA isolates, respectively. Each isolate carried at least six virulence genes. The positive rates of Panton-Valentine leukocidin genes among all S. aureus, MRSA and methicillin-susceptible S. aureus isolates were 30.8% (28/91), 39.0% (16/41) and 24% (12/50), respectively. The prevalence of community-associated MRSA was surprisingly high among children with community-acquired SSTIs in Shanghai. ST59-t437 was the most prevalent community-acquired S. aureus clone causing SSTIs.

scholarly journals Community acquired MRSA infections in a paediatric population in Greece

2005 ◽  
Vol 10 (5) ◽  
pp. 7-8 ◽  
Author(s):  
S Vourli ◽  
D Perimeni ◽  
A Makri ◽  
M Polemis ◽  
A Voyiatzi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Paediatric Population ◽  
Pfge Pattern ◽  
Soft Tissue Infections ◽  
Paediatric Hospital ◽  
Mrsa Strains ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

We investigated the characteristics of 20 community acquired methicillin resistant Staphylococcus aureus (MRSA) strains isolated in a paediatric hospital in Athens. Eighteen of these, all isolated from skin and soft tissue infections, carried the Panton-Valentine leukocidin (PVL) determinants. They all were found resistant to fusidic acid, tetracycline and kanamycin, and displayed a PFGE pattern identical to that of the well-described ST80 CA-MRSA clone circulating in various European countries.

scholarly journals ST93-Queensland community-acquired meticillin-resistant Staphylococcus aureus clone in France: outbreak in a scout camp and sporadic cases, July to August 2012

2012 ◽  
Vol 17 (44) ◽  
Author(s):  
T X Nhan ◽  
M Bes ◽  
H Meugnier ◽  
L Toko ◽  
G Julienne ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Soft Tissue Infections ◽  
Sporadic Cases ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

We describe the occurrence in France of a Panton-Valentine leukocidin (PVL)-positive meticillin-resistant Staphylococcus aureus (MRSA) ST93 clone, a predominant community-acquired (CA)-MRSA in Australia. In July to August 2012, an outbreak in a scout camp (n=3) and sporadic cases (n=2) of skin and soft tissue infections were reported. Investigations suggested importation of the clone through travel and onward transmission. This illustrates the epidemic potential of this lineage and the role of travellers in the spread of PVL-positive CA-MRSA.

scholarly journals Panton–Valentine leukocidin Staphylococcus aureus osteomyelitis of the adult tibia – a case report

2010 ◽  
Vol 92 (2) ◽  
pp. e17-e19 ◽  
Author(s):  
MJ Hall ◽  
JA Steer ◽  
J Keenan
Keyword(s):  
Staphylococcus Aureus ◽  
Case Report ◽  
Soft Tissue ◽  
Surgical Management ◽  
Adult Male ◽  
Soft Tissue Infections ◽  
Musculoskeletal Infection ◽  
Invasive Infections ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Panton–Valentine leukocidin toxin producing Staphylococcus aureus (PVLSA) is known to be responsible for recurrent soft tissue infections and more serious invasive infections including necrotising pneumonia, pyomyositis, and osteomyelitis. Most reported cases involving musculoskeletal infection in adults are associated with methicillin-resistant S. aureus (MRSA) PVL-producing strains. We present the case of an adult male with PVL toxin–producing methicillin-sensitive S. aureus (MSSA) osteomyelitis of the tibia which has not previously been described in adults and highlight issues of recognition, treatment, and surgical management of PVLSA osteomyelitis.

scholarly journals Panton-Valentine Leukocidin (PVL)-Positive Health Care-Associated Methicillin-Resistant Staphylococcus aureus Isolates Are Associated with Skin and Soft Tissue Infections and Colonized Mainly by Infective PVL-Encoding Bacteriophages

2014 ◽  
Vol 53 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Qiwen Hu ◽  
Hang Cheng ◽  
Wenchang Yuan ◽  
Fangyin Zeng ◽  
Weilong Shang ◽  
...  
Keyword(s):  
Health Care ◽  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Health Concern ◽  
Soft Tissue Infections ◽  
Positive Health ◽  
Methicillin Resistant ◽  
Mrsa Strains ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

The emergence of Panton-Valentine leukocidin (PVL)-positive methicillin-resistantStaphylococcus aureus(MRSA) is a public health concern worldwide. PVL is associated with community-associated MRSA and is linked to skin and soft tissue infections (SSTIs). However, PVL genes have also been detected in health care-associated (HA) MRSA isolates. The diseases associated with PVL-positive HA-MRSA isolates and the distributions of PVL-encoding bacteriophages in HA-MRSA have not been determined. In this study, a total of 259 HA-MRSA strains isolated between 2009 and 2012 in China from inpatients with SSTIs, pneumonia, and bacteremia were selected for molecular typing, including staphylococcal cassette chromosomemectyping, multilocus sequence typing, and staphylococcal protein A gene typing. The PVL genes and PVL bacteriophages in the MRSA isolates were characterized by PCR. Among the tested MRSA isolates, 28.6% (74/259) were PVL positive. The high prevalence of PVL-carrying HA-MRSA was observed to be associated with SSTIs but not with pneumonia or bacteremia. The PVL-positive HA-MRSA isolates were colonized mainly by infective PVL phages, namely, Φ7247PVL, ΦSLT, and ΦSa2958. The distribution of PVL-carrying bacteriophages differed geographically. Our study highlights the potential risk of the emergence of multidrug-resistant HA-MRSA strains with increased virulence.

Skin and soft tissue infections due to Panton–Valentine leukocidin producing Staphylococcus aureus

2012 ◽  
Vol 42 (10) ◽  
pp. 488-494 ◽  
Author(s):  
C. Gros ◽  
Y. Yazdanpanah ◽  
A. Vachet ◽  
M. Roussel-Delvallez ◽  
E. Senneville ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Soft Tissue Infections ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

scholarly journals Lateral Flow Immunoassay for the Detection of Panton-Valentine Leukocidin in Staphylococcus aureus From Skin and Soft Tissue Infections in the United Arab Emirates

2021 ◽  
Vol 11 ◽  
Author(s):  
Abiola Senok ◽  
Stefan Monecke ◽  
Rania Nassar ◽  
Handan Celiloglu ◽  
Sreeraj Thyagarajan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
United Arab Emirates ◽  
Dna Microarray ◽  
Fusidic Acid ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Soft Tissue Infections ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

IntroductionPanton Valentine leukocidin (PVL) is a virulence factor which is associated with methicillin sensitive and resistant Staphylococcus aureus (MSSA/MRSA) causing skin and soft tissue infections (SSTI). This study aimed to evaluate a novel lateral flow immunoassay (LFI) for PVL detection in S. aureus cultures and to describe their genotypic characterization.MethodsThe study was carried out from January-August 2020 in Dubai, United Arab Emirates. S. aureus isolates associated with SSTI were tested for PVL detection using LFI. DNA microarray-based assays were used for molecular characterization including detection of pvl genes.ResultsOne-hundred thirty-five patients with a clinical diagnosis of SSTIs were recruited. Sixty-six patients received antibiotics, mostly beta lactams (n=36) and topical fusidic acid (n=15). One-hundred twenty-nine isolates (MRSA: n=43; MSSA: n=86) were tested by LFI and DNA microarrays. All 76 (58.9%) isolates which were unambiguously negative for the PVL in LFI were negative for pvl genes using the DNA microarray. All the LFI PVL positive isolates (n=53) had pvl genes detected. This translates into 100% each for sensitivity, specificity, positive and negative predictive values for the LFI. The LFI typically takes about 15 min inclusive of a 10 min incubation period. Predominant S. aureus clonal complexes (CC) were CC30 (n=18), CC22 (n=13), CC5 (n=12), CC1 (n=11), CC152 (n=8), CC15 (n=7); CC97 (n=7); CC8 and CC20 (n=6 each). Among MRSA, the proportion of pvl-positives (35/43; 81%) was higher than among MSSA (n/N=18/86; 21%). The fusidic acid resistance gene fusC was detected in 14 MRSA (33%) compared to 8 MSSA (9%). A co-carriage of fusC and pvl genes was present in 7 MRSA and in one MSSA.ConclusionLFI shows excellent diagnostic accuracy indices for rapid identification of PVL in MSSA/MRSA in a setting with high prevalence of pvl+ve strains. The high occurrence of pvl and fusC genes in MRSA strains causing SSTI is of concern and needs constant surveillance.

Panton-Valentine Leukocidin Staphylococcus Aureus (PVL-SA) – an uninvited stowaway aboard a Royal Navy submarine

2013 ◽  
Vol 99 (1) ◽  
pp. 3-8
Author(s):  
SJN Bourn ◽  
G Evans ◽  
MK O’Shea
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Virulence Factor ◽  
Invasive Disease ◽  
Soft Tissue Infections ◽  
Royal Navy ◽  
Military Populations ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

AbstractThe toxin Panton-Valentine Leukocidin (PVL) is a virulence factor produced by some strains of Staphylococcus aureus (S. aureus, SA) that destroys leucocytes. PVL-SA predominantly results in skin and soft tissue infections (SSTIs) but may also cause invasive disease, including necrotising pneumonia, which may affect healthy young individuals and has a high mortality. We describe a case of PVL-SA in a submariner who presented at sea and which resulted in a requirement to both alter the submarine’s programme and undertake population screening. The case highlights many important issues pertinent to military populations including clinical and public health considerations.

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