Chemical Composition and Anti-inflammatory Activity of <i>Apium graveolens</i> var. dulce Essential Oils from Senegal

Herba Siegesbeckiae (HS), derived from the aerial parts of three plants, Siegesbeckia orientalis (SO), S. glabrescens (SG), and S. pubescens (SP), has been used for the treatment of inflammatory diseases in China for centuries. In the present study, hydrodistillation was applied to extract essential oils from dried SO, SG, and SP aerial parts, and chemical composition analysis by gas chromatography–mass spectrometry (GC-MS) led to the identification of a total of 148 compounds (56 in SO, 62 in SG, and 59 in SP). The main components in the essential oils of SO, SG, and SP differed significantly. In vitro anti-inflammatory activity assays showed that SP essential oils (IC50, 0.97 μg/mL) significantly reduced the ability of lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages to release NO, and the SO essential oil (IC50, 14.99 μg/mL) was better than the others at inhibiting the LPS-induced release of cytokine IL-6. Furthermore, the essential oils exhibited antitumor activities (IC50, 37.72–123.16 μg/mL) against Hep3B (liver) and Hela (cervical) cells. Linear regression analysis showed that, caryophyllene oxide peak area percentages showed remarkably high negative correlation coefficients with IC50 values of Hep3B and Hela cytotoxicity, which suggested the contribution of this compound on the cancer cell cytotoxicity of three essential oils. Finally, the ITS1-5.8S-ITS2 region was amplified and sequenced in order to generate genomic reference sequences for each plant. These can be used to identify the origins of the plants, and will assist other research studies related to these three plants.

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Chemical Composition, Antioxidant, Antimicrobial and Anti-inflammatory Activities of the Stem and Leaf Essential Oils from Piper flaviflorum from Xishuangbanna, SW China

Natural Product Communications ◽

10.1177/1934578x1400900732 ◽

2014 ◽

Vol 9 (7) ◽

pp. 1934578X1400900 ◽

Cited By ~ 1

Author(s):

Ren Li ◽

Jing-jing Yang ◽

Yuan-fei Wang ◽

Qian Sun ◽

Hua-bin Hu

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Positive Control ◽

Inflammatory Activity ◽

Raw 264.7 Cells ◽

No Production ◽

Raw 264.7 ◽

Sw China ◽

Comparable Activity ◽

Anti Inflammatory

The present study is the first investigation of the chemical composition, antioxidant, antimicrobial and anti-inflammatory activities of the stem and leaf essential oils from Piper flaviflorum C.DC (SEOP and LEOP), a plant that has been consumed as a wild vegetable, and used as medicine, and spice by the ethnic groups in Xishuangbanna, SW China. Analyzed by GC-MS, 42 and 30 components were identified representing 90.1% and 95.3% of the SEOP and LEOP, with (E)-nerolidol (16.7% and 40.5%), β-caryophyllene (26.6% and 14.6%) and elixene (5.3% and 12.3%) as their main constituents, respectively. Our results indicate that SEOP and LEOP have good anti-inflammatory activity by significantly inhibiting NO production induced by LPS in RAW 264.7 cells at 0.04± without effect on cell viability, and negligible antioxidant activity in both ABTS and FRAP assays. Moreover, the LEOP showed comparable activity with the positive control (tigecycline) against Aspergillus fumigatus, with MIC and MBC values ranging from 256 to 1024 μg/mL. The anti-inflammatory activity in LPS-induced RAW 264.7 cells is worthy of further investigation to discover the possible mechanisms of the NO production inhibition effect of these essential oils.

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Chemical Composition and In vivo Anti-inflammatory Activity of Essential Oils from Citrus sinensis (L.) osbeck Growing in South Africa

Journal of Essential Oil Bearing Plants ◽

10.1080/0972060x.2020.1819885 ◽

2020 ◽

Vol 23 (4) ◽

pp. 638-647 ◽

Cited By ~ 1

Author(s):

Thandiswa Matuka ◽

Opeoluwa Oyedeji ◽

Mavuto Gondwe ◽

Adebola Oyedeji

Keyword(s):

South Africa ◽

Chemical Composition ◽

Essential Oils ◽

Citrus Sinensis ◽

Inflammatory Activity ◽

Anti Inflammatory

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Chemical composition and anti-inflammatory activities of the essential oils fromAcacia mearnsiide Wild

Natural Product Research ◽

10.1080/14786419.2014.983504 ◽

2014 ◽

Vol 29 (12) ◽

pp. 1184-1188 ◽

Cited By ~ 8

Author(s):

Opeyemi N. Avoseh ◽

Ope-oluwa O. Oyedeji ◽

Kayode Aremu ◽

Benedicta N. Nkeh-Chungag ◽

Sandile P. Songca ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Anti Inflammatory

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Antipyretic, Analgesic and Anti-inflammatory Activity of Qurs Afsanteen Saghir (A Polyherbal Unani Tablet) in Experimental Animals

Traditional and Integrative Medicine ◽

10.18502/tim.v6i4.8265 ◽

2022 ◽

Author(s):

MD Maseehullah ◽

Gulam Mohammed Husain ◽

Mohammed Zakir ◽

Mohd Kashif Husain ◽

Ghazala Javed ◽

...

Keyword(s):

Diclofenac Sodium ◽

Study Drug ◽

Prunus Dulcis ◽

Inflammatory Activity ◽

Apium Graveolens ◽

Dependent Manner ◽

Test Drug ◽

Artemisia Absinthium ◽

Hot Plate Test ◽

Anti Inflammatory

Qurs Afsanteen Saghir is a polyherbal Unani formulation in the form of tablet. This formulation consists of multiple medicinal plants like Afsanteen (Artemisia absinthium L.), Badam Talkh (Prunus dulcis (Mill.) D.A.Webb), Asaroon (Asarum europaeum L.), Anisoon (Pimpinella anisum L.) and Tukhm-e-Karafs (Apium graveolens L.). The clinical adult dose of study drug is 3.5 –7 g per day as mentioned in Unani literature. The present study evaluated the antipyretic, analgesic and anti-inflammatory potential of Qurs Afsanteen Saghir using different animal models. Antipyretic activity was measured using yeast-induced pyrexia model in rats at 360 and 720 mg/kg bw dose of test drug and paracetamol (70 mg/kg bw p.o.) as standard control. Analgesic effect was evaluated using acetic acid-induced writhing test in mice using test drug at dose 720 and 1440 mg/kg bw and diclofenac sodium (15 mg/kg bw p.o.) as standard control. Eddy’s hot plate test was conducted in rats using test drug at the dose of 360 and 720 mg/kg bw and buprenorphine (0.10 mg/kg s.q.) as standard control. Anti-inflammatory activity was assessed by carrageenan-induced paw edema model in rats with the dose of 360 and 720 mg/kg of test drug and Indomethacin (10 mg/kg p.o.) as standard control. The study drug significantly reduced the temperature and pain at both dose levels in a time-dependent manner as compared to normal control. However, the reduction of inflammation was observed at low dose (360 mg/kg bw) only after 3 hours of carrageenan administration. These findings indicated that tested drug showed potential activity as antipyretic and analgesic; whereas the drug may not be considered quite effective as an anti-inflammatory agents.

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