Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a <i>japonica</i> × <i>indica</i> cross

Abstract Background: Rice varieties are required to have high yield and good grain quality. Grain chalkiness and grain shape are two important traits of rice grain quality. Low chalkiness slender grains are preferred by most rice consumers. Here, we dissected two closely linked quantitative trait loci (QTLs) controlling grain chalkiness and grain shape on rice chromosome 8 by substitution mapping. Results: Two closely linked QTLs controlling grain chalkiness and grain shape were identified using single-segment substitution lines (SSSLs). The two QTLs were then dissected on rice chromosome 8 by secondary substitution mapping. qPGC8.1 was located in an interval of 1382.6 kb and qPGC8.2 was mapped in a 2057.1 kb region. The maximum distance of the two QTLs was 4.37 Mb and the space distance of two QTL intervals was 0.72 Mb. qPGC8.1 controlled grain chalkiness and grain width. qPGC8.2 was responsible for grain chalkiness and for grain length and grain width. The additive effects of qPGC8.1 and qPGC8.2 on grain chalkiness were not affected by heat stress. Conclusions: Two closely linked QTLs qPGC8.1 and qPGC8.2 were dissected on rice chromosome 8. They controlled the phenotypes of grain chalkiness and grain shape. The two QTLs were insensitive to high temperature.

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QTL analysis on rice grain appearance quality, as exemplifying the typical events of transgenic or backcrossing breeding

Breeding Science ◽

10.1270/jsbbs.64.231 ◽

2014 ◽

Vol 64 (3) ◽

pp. 231-239 ◽

Cited By ~ 8

Author(s):

Bao Yan ◽

Rongjia Liu ◽

Yibo Li ◽

Yan Wang ◽

Guanjun Gao ◽

...

Keyword(s):

Qtl Analysis ◽

Rice Grain ◽

Appearance Quality

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Whole-Genome Sequencing Identifies a Rice Grain Shape Mutant, gs9–1

Rice ◽

10.1186/s12284-019-0308-8 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 2

Author(s):

Liangrong Jiang ◽

Guotian Li ◽

Mawsheng Chern ◽

Rashmi Jain ◽

Nhan T. Pham ◽

...

Keyword(s):

Whole Genome Sequencing ◽

Genome Sequencing ◽

Grain Shape ◽

Rice Grain ◽

Whole Genome

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Research on Rice Grain Shape Detection Method Based on Machine Vision

2019 5th International Conference on Control, Automation and Robotics (ICCAR) ◽

10.1109/iccar.2019.8813449 ◽

2019 ◽

Author(s):

Yadan Hu ◽

Yunming Du ◽

Linna San ◽

Jing Tian

Keyword(s):

Machine Vision ◽

Grain Shape ◽

Detection Method ◽

Rice Grain ◽

Shape Detection

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Novel OsGRAS19 mutant, D26, positively regulates grain shape in rice (Oryza sativa)

Functional Plant Biology ◽

10.1071/fp18266 ◽

2019 ◽

Vol 46 (9) ◽

pp. 857 ◽

Cited By ~ 2

Author(s):

Zhimin Lin ◽

Jingwan Yan ◽

Jun Su ◽

Huaqing Liu ◽

Changquan Hu ◽

...

Keyword(s):

Functional Analysis ◽

Grain Size ◽

Transcription Factor ◽

Grain Shape ◽

Rice Yield ◽

Reproductive Development ◽

Cell Number ◽

Rice Grain ◽

Regulatory Effect ◽

Site Mutation

Grain size is an important factor in rice yield. Several genes related to grain size have been reported, but most of them are determined by quantitative trail loci (QTL) traits. Gene D26 is a novel site mutation of OsGRAS19 and involved in the brassinosteroid (BR) signalling pathway. However, whether D26 is involved in the process of rice reproductive development remains unclear. Here, gene cloning and functional analysis revealed that D26 has an obvious regulatory effect on grain size. Overexpression or CRISP/Cas9 mutant of D26 also showed that grain size was positively influenced. Cellular analyses show that D26 modulates grain size by promoting cell division and regulating the cell number in the upper epidermis of the glume. The overexpression results further suggest that the level of D26 expression positively impacts grain length and leaf angles and that the expression of several known grain size genes is involved in the regulation. Based on our results, D26, as a transcription factor, effectively improves rice grain shape.

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