scholarly journals Gene Cloning and Characterization of α-Amino Acid Ester Acyl Transferase inEmpedobacter brevisATCC14234 andSphingobacterium siyangensisAJ2458

2011 ◽  
Vol 75 (11) ◽  
pp. 2087-2092 ◽  
Author(s):  
Isao ABE ◽  
Seiichi HARA ◽  
Kenzo YOKOZEKI
Keyword(s):  
Amino Acid ◽  
Gene Cloning ◽  
Acid Ester ◽  
Amino Acid Ester ◽  
Acyl Transferase ◽  
Gene Cloning And Characterization

A novel route for aspartame production by combining enzymatic and chemical reactions for industrial use

2020 ◽  
Vol 85 (2) ◽  
pp. 464-466
Author(s):  
Kenzo Yokozeki ◽  
Isao Abe
Keyword(s):  
Aqueous Solution ◽  
Amino Acid ◽  
Chemical Reactions ◽  
Acid Ester ◽  
Amino Acid Ester ◽  
Acyl Transferase ◽  
Enzymatic Production ◽  
Production Route ◽  
Industrial Use ◽  
Hcl Removal

ABSTRACT Here, we report a novel industrial aspartame production route, involving the enzymatic production of α-l-aspartyl-l-phenylalanine β-methylester from l-aspartic acid dimethylester and l-phenylalanine by α-amino acid ester acyl transferase. The route also involves the chemical transformation of α-l-aspartyl-l-phenylalanine β-methylester to α-l-aspartyl-l-phenylalanine methylester hydrochloride (aspartame hydrochloride) in an aqueous solution with methanol and HCl, followed by HCl removal to form aspartame.

Design and Characterization of Fatty Acid-Based Amino Acid Ester as a New “Green” Hydrophobic Ionic Liquid for Drug Delivery

2020 ◽  
Vol 8 (36) ◽  
pp. 13660-13671 ◽  
Author(s):  
Rahman Md Moshikur ◽  
Md. Raihan Chowdhury ◽  
Hiroki Fujisawa ◽  
Rie Wakabayashi ◽  
Muhammad Moniruzzaman ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Ionic Liquid ◽  
Fatty Acid ◽  
Amino Acid ◽  
Acid Ester ◽  
Amino Acid Ester ◽  
Hydrophobic Ionic Liquid

Eco-Friendly Synthesis of Peptides Using Fmoc-Amino Acid Chlorides as Coupling Agent Under Biphasic Condition

2020 ◽  
Vol 27 ◽  
Author(s):  
Santosh Y. Khatavi ◽  
K. Kantharaju
Keyword(s):  
Amino Acid ◽  
Acid Chloride ◽  
Acid Ester ◽  
Analytical Techniques ◽  
Peptide Bond ◽  
Amino Acid Ester ◽  
Acid Chlorides ◽  
Peptide Bond Formation ◽  
Green Protocol ◽  
Additional Base

Background: Agro-waste derived solvent media act as a greener process for the peptide bond formation using Nα - Fmoc-amino acid chloride and amino acid ester salt with in situ neutralization and coupling under biphasic condition. The Fmoc-amino acid chlorides are prepared by the reported procedure of freshly distilled SOCl2 with dry CH2Cl2. The protocol found many added advantages such as neutralization of amino acid ester salt and not required additional base for the neutralization, and directly coupling take place with Fmoc-amino acid chloride gave final product dipeptide ester in good to excellent yields. The protocol occurs with complete stereo chemical integrity of the configuration of substrates. Here, we revisited Schotten-Baumann condition, instead of using inorganic base. Objective: To develop green protocol for the synthesis of peptide bond using Fmoc-amino acid chloride with amino acid esters salt. Methods: The final product isolated is analyzed in several spectroscopic and analytical techniques such as FT-IR, 1H-, 13CNMR, Mass spectrometry and RP-HPLC to check stereo integrity and purity of the product. Conclusion: The present method developed greener using natural agro-waste (lemon fruit shell ash) derived solvent medium for the reaction and not required chemical entity.

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