Structure of a New Antifungal C11-Hydroxyfatty Acid Isolated from Leaves of Wild Rice (Oryza officinalis)

A comparative RFLP map was constructed in a wild rice, Oryza officinalis, by using 139 genomic and cDNA probes that had been used previously to map RFLPs in O. sativa. Nine of the 12 chromosomes of O. officinalis were highly homosequential to those of O. sativa. A major rearrangement of gene order was detected in chromosome 1 and small inversions were found in chromosomes 3 and 11. Fourteen translocated RFLP markers were found, and chromosome 11 contained a high frequency of such translocated segments. Results were consistent with meiotic and trisomie analysis, which suggested that the genomes of O. officinalis and O. sativa were similar. Applications of comparative maps in plant breeding and gene cloning are discussed.Key words: Oryza, rice, wild rice, RFLP, genetic map.

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Complex arrangement of dispersed repeated DNA sequences in Oryza officinalis

Genome ◽

10.1139/g96-024 ◽

1996 ◽

Vol 39 (1) ◽

pp. 183-190 ◽

Cited By ~ 4

Author(s):

M. C. Kiefer-Meyer ◽

A. S. Reddy ◽

M. Delseny

Keyword(s):

Dna Sequences ◽

Wild Rice ◽

Repetitive Sequences ◽

Rice Genome ◽

Repeated Dna ◽

Element Analysis ◽

Oryza Officinalis ◽

Repeated Dna Sequences ◽

Genomic Clones ◽

Complex Arrangement

A 525-bp BglII fragment was isolated from Oryza officinalis DNA (accession W1278) and shown to correspond to a new dispersed repetitive DNA sequence with specificity restricted to a subset of the wild rice with a C genome. The sequence of the fragment was determined but it does not correspond to any sequence already present in databases. It contains several imperfect palindromes. Larger genomic clones (12–18 kbp) were isolated and all contain sequences homologous to the BglII element. Analysis of these clones confirms that the BglII element is dispersed in the O. officinalis genome. From one genomic clone, the sequences adjacent to the BglII element were subcloned and used as probes to demonstrate that the sequences flanking the BglII element are variable in different genomic clones and that some of them are also dispersed repetitive sequences. The genomic specificity of two of these dispersed repeats was evaluated and shown to be different from that of the initial BglII element. This analysis revealed a complex arrangement of various dispersed repeated sequences. Key words : wild rice, genome specificity, repeated DNA sequences.

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Small brown planthopper resistance loci in wild rice (Oryza officinalis)

Molecular Genetics and Genomics ◽

10.1007/s00438-014-0814-8 ◽

2014 ◽

Vol 289 (3) ◽

pp. 373-382 ◽

Cited By ~ 14

Author(s):

Weilin Zhang ◽

Yan Dong ◽

Ling Yang ◽

Bojun Ma ◽

Rongrong Ma ◽

...

Keyword(s):

Wild Rice ◽

Brown Planthopper ◽

Oryza Officinalis ◽

Small Brown Planthopper ◽

Brown Planthopper Resistance

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Characterization of new variants of a satellite DNA from Oryza officinalis, specific for the CC genome of wild rice

Genome ◽

10.1139/g93-100 ◽

1993 ◽

Vol 36 (4) ◽

pp. 750-761 ◽

Cited By ~ 9

Author(s):

A. S. Reddy ◽

M. C. Kiefer-Meyer ◽

M. Delseny

Keyword(s):

Satellite Dna ◽

Wild Rice ◽

Repeated Sequence ◽

Addition Lines ◽

Oryza Officinalis ◽

Monosomic Addition ◽

Monosomic Addition Lines ◽

The Relationship ◽

Allotetraploid Species

Two groups of tandemly repeated DNA fragments have been isolated and cloned from the Oryza officinalis genome (W 1278). These fragments have been sequenced and are 374 and 367 bp long. They were compared with an element previously isolated from another O. officinalis accession. They are 76 and 92% homologous to the latter and show 78% homology between themselves. The two types of elements are interspersed within large blocks of tandemly organized units. Their copy number is in the range of 200 000 units. This sequence was present only in wild rice accessions with a CC genome. However, this apparent specificity is even more restricted, since it was absent from some O. officinalis accessions and from the allotetraploid species of the CCDD type. Use of digestions with 4-bp restriction enzyme cutters and analysis on denaturing polyacrylamide gels revealed polymorphism that might be used to map the repeats in the genome as well as to establish the relationship between accessions. Finally, hybridization of the repeated sequence to DNA prepared from the eight available O. sativa – O. officinalis monosomic addition lines revealed that the elements are located on two alien chromosomes. This suggests that these elements have at least some restricted chromosome specificity.Key words: satellite DNA, rice, monosomic addition lines, RFLP, evolution.

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