scholarly journals Improvement of GFPuv-β3GnT2 Fusion Protein Production by Suppressing Protease in Baculovirus Expression System

2003 ◽  
Vol 67 (11) ◽  
pp. 2388-2395 ◽  
Author(s):  
Tatsuya KATO ◽  
Takeomi MURATA ◽  
Taichi USUI ◽  
Enoch Y PARK
Keyword(s):  
Fusion Protein ◽  
Protein Production ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Baculovirus Expression

Engineering of the baculovirus expression system for optimized protein production

2018 ◽  
Vol 103 (1) ◽  
pp. 113-123 ◽  
Author(s):  
María Martínez-Solís ◽  
Salvador Herrero ◽  
Alexandra M. Targovnik
Keyword(s):  
Protein Production ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Baculovirus Expression

scholarly journals Expression, purification and structural analysis of functional GABA transporter 1 using the baculovirus expression system

2017 ◽  
Vol 13 ◽  
pp. 874-882 ◽  
Author(s):  
Jing Hu ◽  
Chris Weise ◽  
Christoph Böttcher ◽  
Hua Fan ◽  
Jian Yin
Keyword(s):  
Fusion Protein ◽  
Fluorescent Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Size Exclusion ◽  
Coupled Transport ◽  
Gaba Transporter ◽  
Gfp Fusion Protein ◽  
Baculovirus Expression ◽  
Gaba Transporter 1

The γ-aminobutyric acid (GABA) transporter 1 (GAT1) belongs to a family of Na+ and Cl−-coupled transport proteins and possesses 12 putative transmembrane domains. To perform structural analyses of the GAT1 protein, the GAT1/green fluorescent protein (GFP) fusion protein was functionally expressed in insect Sf9 cells by the BAC-TO-BAC® baculovirus expression system. A two-step procedure to purify the GAT1/GFP fusion protein from insect Sf9 cells has been established and involves immunoaffinity chromatography using self-prepared anti-GFP antibodies and size-exclusion fast protein liquid chromatography (SE-FPLC). A yield of 200–300 μg of the GAT1/GFP protein could be purified from 400–600 mL of infected Sf9 cells. The purified protein was analyzed by transmission electron microscopy (TEM), which revealed that the GAT1/GFP fusion protein was isolated in its monomeric form.

Production of a urokinase plasminogen activator-IgG fusion protein (uPA-IgG) in the baculovirus expression system

Gene ◽  
1997 ◽  
Vol 190 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Thomas A. Kost ◽  
Diane M. Ignar ◽  
William C. Clay ◽  
John Andrews ◽  
Jeremy D. Leray ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Plasminogen Activator ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Urokinase Plasminogen Activator ◽  
Baculovirus Expression

Protein Production using the Baculovirus Expression System

2018 ◽  
Vol 91 (1) ◽  
Author(s):  
Sarah L. Irons ◽  
Adam C. Chambers ◽  
Olga Lissina ◽  
Linda A. King ◽  
Robert D. Possee
Keyword(s):  
Protein Production ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Baculovirus Expression
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