scholarly journals Effects of Feeder Cell Types on Culture of Mouse Embryonic Stem Cell In Vitro

2015 ◽  
Vol 19 (3) ◽  
pp. 119-126 ◽  
Author(s):  
Yun-Gwi Park ◽  
Seung-Eun Lee ◽  
Eun-Young Kim ◽  
Hyuk Hyun ◽  
Min-Young Shin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Cell Types ◽  
Feeder Cell

scholarly journals Spontaneous induction of an homologous Robertsonian translocation, Rb(11.11) in a murine embryonic stem cell line

1990 ◽  
Vol 55 (2) ◽  
pp. 107-110 ◽  
Author(s):  
John Anthony Crolla ◽  
David Brown ◽  
David G. Whittingham
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Recombinant Dna ◽  
Genetic Manipulation ◽  
Embryonic Stem Cell Line ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Transgenic Animals ◽  
Translocation Chromosome

SummaryKaryotype analysis of a series of established mouse embryonic stem cell (MESC) lines showed that the majority were aneuploid by the 7th and 9th passage and that all lines contained a single Robertsonian (Rb) translocation chromosome with a symmetrical, homologous, arm composition Rb(11.11). Although the chromosomal imbalance makes these MESC lines unsuitable for genetic manipulation in vitro and hence for subsequent production of transgenic animals, the spontaneous occurrence and stable retention of the homologous Rb(11.11) as the only metacentric chromosome in an otherwise all acrocentric karyotype, provides potentially useful cell lines for gene assignment and recombinant DNA studies.

Simvastatin modulates mouse embryonic stem cell-derived chondrogenesis in vitro

2012 ◽  
Vol 26 (7) ◽  
pp. 1170-1176 ◽  
Author(s):  
J. Kramer ◽  
M. Bartsch ◽  
D. Krug ◽  
M. Klinger ◽  
M. Nitschke ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell

scholarly journals Asymptomatic neurotoxicity of amyloid β-peptides (Aβ1-42 and Aβ25-35) on mouse embryonic stem cell-derived neural cells

2020 ◽  
Author(s):  
Nur Izzati Mansor ◽  
Carolindah Makena Ntimi ◽  
Noraishah Mydin Abdul-Aziz ◽  
King-Hwa Ling ◽  
Aishah Adam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Neural Cells ◽  
Aβ Peptides ◽  
Intracellular Ros

One of the strategies in the establishment of in vitro oxidative stress models for neurodegenerative diseases, such as Alzheimer’s disease (AD), is to induce neurotoxicity by amyloid beta (Aβ) peptides in suitable neural cells. Presently, data on the neurotoxicity of Aβ in neural cells differentiated from stem cells are limited. In this study, we attempted to induce oxidative stress in transgenic 46C mouse embryonic stem cell-derived neurons via treatment with Ab peptides (Aβ1-42 and Aβ25-35). 46C neural cells were generated by promoting the formation of multicellular aggregates, embryoid bodies (EBs) in the absence of leukemia inhibitory factor (LIF), followed by the addition of all-trans retinoic acid (ATRA) as the neural inducer. Mature neuronal cells were exposed to different concentrations of Aβ1-42 and Aβ25-35 for 24 h. Morphological changes, cell viability, and intracellular ROS production were assessed. We found that 100 µM Aβ1-42 and 50 µM Aβ25-35 only promoted 40% and 10%, respectively, of cell injury and death in the 46C-derived neuronal cells. Interestingly, treatment with each of the Aβ peptides resulted in a significant increase of intracellular ROS activity, as compared to untreated neurons. These findings indicate the potential of using neurons derived from stem cells and Aβ peptides in generating oxidative stress for the establishment of an in vitro AD model that could be useful for drug screening and natural product studies.

Indirect immune recognition of mouse embryonic stem cell–derived hematopoietic progenitors in vitro

2014 ◽  
Vol 42 (5) ◽  
pp. 347-359.e5 ◽  
Author(s):  
Heather L. Thompson ◽  
Bryce T. McLelland ◽  
Jennifer O. Manilay
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Immune Recognition ◽  
Hematopoietic Progenitors
Sign in / Sign up

Export Citation Format

Share Document