Efficiency of in vivo Embryo Production in Elite Hanwoo and Embryo Transfer

Abstract BackgroundThe success of an embryo transfer protocol in sheep depends on many factors, but the choice of drugs for the desired superovulation as well as the conception rate are most essential. Reproductive activity in sheep is characterized by a seasonality influenced by several factors such as photoperiod, latitude, temperature, nutrition and breed. Reproductive seasonality and nutritional condition are the main factors that influence embryo production in sheep. In sheep, some anatomical peculiarities limit the application of traditional reproductive biotechnologies used in cattle. MethodsIn vivo embryo production is often referred to as “multiple ovulation and embryo transfer” and involves ovarian superstimulation of the donor female, insemination or mating, uterine flushing for embryo recovery, and either cryopreservation or transfer of collected embryos to recipients. A total number of 60 sheep and 3 rams were included in this study, divided into 2 groups (receptors/donors). Donor Suffolk sheep were treated for superovulation using the P4‐PGF‐FSH protocol while the cross-bred recipients’ group was synchronized with P4-PGF-PMSG. ResultsOn the first day after superovulation, all ovaries had more than 5 dominant follicles, while corpora lutea were later observed in 83.3% sheep. The recovery rate was 83.3% while 72,9% embryos were transferable. Embryos were transferred directly into recipients. Fertility after 30 days was 68.57%, lambing rate was 91.6%, and CR 62.85%. This study showed that veterinary drugs (P4, FSH, LH, PMSG, PGF) used for superovulation were capable to induce estrus and synchronize ovulation in sheep, are topical and in increasing use worldwide. ConclusionsThe aim of this study was to conclude on the effectiveness of a wider on farm in vivo embryo transfer development program in Suffolk sheep, using several veterinary hormones. The application of a multiple ovulation embryo transfer (MOET) protocol has a positive effect in the production of in vivo derived embryos in Suffolk sheep and can guarantee the success of embryo transfer activity to ewes with lower genetic merit. Our research aimed at representing a model for sheep farms for a rapid improvement of productive traits.

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ECONOMIC PERSPECTIVES OF BIOTECHNOLOGIES USING IN ANIMAL FARMING

Vestnik of the Russian agricultural science ◽

10.30850/vrsn/2019/5/57-60 ◽

1970 ◽

pp. 57-60

Author(s):

I. F. Gorlov ◽

A. A. Mosolov ◽

G. V. Komlatskiy ◽

M. A. Nesterenko ◽

K. D. Nimbona ◽

...

Keyword(s):

Embryo Transfer ◽

Economic Effect ◽

Dairy Herd ◽

State Level ◽

Modern Level ◽

Economic Perspectives ◽

The Cost ◽

Short Time

The article presents materials on the study of the possibility of reproduction and increase in the herd of highly productive cows through the use of embryo transplantation technology. The classical (in vivo) and more modern, developing (in vitro) methods of embryotransfer, their positive and negative sides are considered in detail. The possibility of accelerating the breeding process by using the method of transplantation, in which from one cow can be obtained from 10 to 100 calves, which will allow for 4-5 years, almost any herd (of any size and breed) with the help of biotechnology to turn into a cattle-breeding enterprise of the most modern level. At the same time, heifers obtained from unproductive cows can be used as "surrogate" mothers who are transplanted with the best donor embryos, which allows to obtain a full-fledged offspring adapted to local environmental conditions. A detailed scheme of obtaining, evaluation, storage, as well as the cost and economic effect of embryo transplantation was calculated, the market was evaluated, the required annual volume of transplants and the number of donor cows for large livestock farms were determined. As a positive example of "Scientific-production enterprise "Centre of biotechnology and embryo transfer" in 2014, implemented a project for accelerated replacement and genetic improvement of the dairy herd, engraftment averaged 57-69%, and the economic effect of the enterprise from getting a single animal by the method of embryo transfer, compared with imports of similar close in quality, ranged from 60 to 100 thousand rubles on his head. It is shown that it is necessary to organize at the state level a developed service for embryo transplantation to reduce the cost of embryo transfer and the possibility of creating in a short time in the country's own highly productive breeding nucleus of dairy and beef cattle, which will reduce, and in the future completely eliminate, import dependence on cattle products.

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Effects of rate of development of in vitro-produced ovine embryos on sex ratio and in vivo survival after embryo transfer

Theriogenology ◽

10.1016/s0093-691x(97)00378-6 ◽

1997 ◽

Vol 48 (8) ◽

pp. 1369-1378 ◽

Cited By ~ 14

Author(s):

S.L. Catt ◽

J.K. O'Brien ◽

W.M.C. Maxwell ◽

G. Evans

Keyword(s):

Sex Ratio ◽

Embryo Transfer ◽

Rate Of Development

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Response of Bali cows on superovulation for in-vivo embryo production

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/465/1/012046 ◽

2020 ◽

Vol 465 ◽

pp. 012046

Author(s):

Muhammad Yusuf ◽

Hasbi ◽

Abd.Latief Toleng ◽

Herry Sonjaya ◽

Sahiruddin

Keyword(s):

Embryo Production

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The effect of melatonin and season on in vivo embryo production of Dohne Merino ewes

Small Ruminant Research ◽

10.1016/j.smallrumres.2014.05.003 ◽

2014 ◽

Vol 120 (1) ◽

pp. 121-124 ◽

Cited By ~ 5

Author(s):

A. Buffoni ◽

P.A. Vozzi ◽

D.M. Gonzalez ◽

G. Rios ◽

H. Viegas-Bordeira ◽

...

Keyword(s):

Embryo Production

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In vivo and in vitro embryo production in goats

Small Ruminant Research ◽

10.1016/j.smallrumres.2009.12.037 ◽

2010 ◽

Vol 89 (2-3) ◽

pp. 144-148 ◽

Cited By ~ 19

Author(s):

M.T. Paramio

Keyword(s):

Embryo Production ◽

In Vitro Embryo Production

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132 SUCCESSFUL EMBRYO TRANSFER OF IN VIVO-PRODUCED RED DEER (CERVUS ELAPHUS) EMBRYOS AFTER CRYOPRESERVATION BY SLOW FREEZING OR VITRIFICATION

Reproduction Fertility and Development ◽

10.1071/rdv19n1ab132 ◽

2007 ◽

Vol 19 (1) ◽

pp. 183

Author(s):

J. P. Soler ◽

G. G. Kaiser ◽

N. Mucci ◽

L. B. Ferre ◽

R. H. Alberio

Keyword(s):

Liquid Nitrogen ◽

Embryo Transfer ◽

Cervus Elaphus ◽

Red Deer ◽

Slow Freezing ◽

Alternative Methods ◽

Embryo Cryopreservation ◽

Chi Square ◽

Cryopreserved Embryos

Multiple ovulation and embryo transfer (MOET) programs for red deer (Cervus elaphus) have been established commercially over the last decade, with embryo cryopreservation being a related practice necessary to enhance the use of valuable genetic information. The aim of this work was to establish alternative methods for red deer embryo cryopreservation by using slow freezing with ethylene glycol (SF–EG) and vitrification by open pulled straw (OPS) methods. After surgical flushing of 18 superstimulated donors, 54 transferable embryos were recovered; 28 were transferred fresh to synchronized recipients and the others were cryopreserved by SF–EG (n = 11) or OPS (n = 15), respectively thawed or warmed, and transferred to recipients. Fresh embryos were maintained in Dulbecco's PBS + 20% cow serum (holding medium, HM) until transfer (maximum 3 h after collection). SF–EG cryopreserved embryos were suspended in HM + 1.78 M EG + 0.1 M sucrose + 4 mg mL−1 BSA. After a 10-min equilibration, embryos were loaded individually into 0.25-mL plastic straws and placed into a −7°C methanol bath chamber. After seeding (5 min later), the straws were cooled from −7 to −35°C at a rate of 0.5°C min. Straws were plunged into and stored in liquid nitrogen. Thawing was performed by placing the straws in a 30°C water bath for 30 s; their contents were drained into HM until transfer. Embryos were vitrified using the OPS method with minor modifications. They were first incubated in HM + 1.78 M EG + 1.3 M DMSO for 3 min and then transferred for 25 s into a vitrification solution of HM + 3.56 M EG + 2.6 M DMSO + 0.5 M sucrose. Each embryo was loaded by touching a 1-µL drop with the straw, which was immediately submerged into and stored in liquid nitrogen. Warming was done by placing the narrow end of the straws into HM + 0.25 M sucrose for 5 min. Embryos were then transferred into HM + 0.15 M sucrose for 5 min and finally to HM until transfer. Both types of cryopreserved embryos were transferred a few hours after collection, immediately after thawing or warming. Before embryo transfer, the presence of corpus luteum (CL) of recipients was confirmed by laparoscopic examination. Each embryo was surgically transferred into the apical extreme of the uterine horn ipsilateral to the CL of one recipient. Pregnancy was determined by ultrasonography 41 days after embryo transfer. The pregnancy rate between groups was compared with the chi-square test (P < 0.05). No statistical differences were found between groups (Table 1). Our results show that both vitrification and slow freezing methods with EG are suitable to cryopreserve red deer embryos. Table 1. Pregnancy rates in recipient hinds after transfer of fresh, vitrified, or frozen red deer embryos

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