scholarly journals Change in VEGF Expression in Mouse Mammary Gland during Reproductive Cycle

2010 ◽  
Vol 72 (9) ◽  
pp. 1159-1163 ◽  
Author(s):  
Mohammad Saiful ISLAM ◽  
Mitsuharu MATSUMOTO ◽  
Rina ISHIDA ◽  
Tatsuzo OKA ◽  
Hiroaki KANOUCHI
Keyword(s):  
Mammary Gland ◽  
Reproductive Cycle ◽  
Mouse Mammary Gland ◽  
Vegf Expression

scholarly journals Reproductive Cycle Regulation of Nuclear Import, Euchromatic Localization, and Association with Components of Pol II Mediator of a Mammalian Double-Bromodomain Protein

2002 ◽  
Vol 16 (8) ◽  
pp. 1727-1737 ◽  
Author(s):  
Thomas E. Crowley ◽  
Emily M. Kaine ◽  
Manabu Yoshida ◽  
Anindita Nandi ◽  
Debra J. Wolgemuth
Keyword(s):  
Mammary Gland ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Reproductive Cycle ◽  
Large Subunit ◽  
Mouse Mammary Gland ◽  
Receptor Associated Protein ◽  
E2f Transcription Factor ◽  
Mammary Epithelia ◽  
Gland Function

Abstract Fsrg1 (female sterile homeotic-related gene 1) is the mouse homolog of the human RING3 protein, which has been shown to associate with the E2 promoter binding factor (E2F) transcription factor and to have a possible role in cell cycle-linked transcriptional regulation. The Fsrg1 protein is 60% identical in sequence to the RNA polymerase II mediator subunit Fsrg4, another member of this subfamily of double bromodomain-containing proteins that are homologs of Drosophila female sterile homeotic. Antibodies against murine Fsrg1 were generated and used in immunoblot and immunoprecipitation experiments to identify proteins interacting with Fsrg1 and RING3. In the presence of acetylated but not nonacetylated histone H3 and H4 peptides, RING3 was shown to interact with E2F, mediator components cyclin-dependent kinase 8 and thyroid receptor-associated protein 220, and the RNA polymerase II large subunit. Fsrg1 mRNA had been previously shown to be expressed at high levels in the epithelium of the adult mouse mammary gland. To determine the physiological relevance of these potential associations, we examined the patterns of expression of Fsrg1 mRNA and protein in the adult mammary epithelia during the reproductive cycle as the tissue is responding to estrogen, progesterone, and prolactin. Changes in the nuclear vs. cytoplasmic localization of Fsrg1 were observed and correlated with physiological changes in mammary gland function. The observations suggested that Fsrg1 may be involved in the transcriptional activities of genes involved in proliferation of the mammary epithelia during pregnancy and in orchestrating postlactation involution and apoptosis. Localization of Fsrg1 on euchromatin, the transcribed portion of the chromosomes, is consistent with its hypothesized function as a transcription regulator.

scholarly journals Immunohistochemical Localization of Nitric Oxide Synthase (NOS) in Mouse Mammary Gland during Reproductive Cycle

2009 ◽  
Vol 71 (7) ◽  
pp. 945-949 ◽  
Author(s):  
Mohammad Saiful ISLAM ◽  
Mitsuharu MATSUMOTO ◽  
Kaze TSUCHIDA ◽  
Tatsuzo OKA ◽  
Hiroaki KANOUCHI ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mammary Gland ◽  
Nitric Oxide Synthase ◽  
Reproductive Cycle ◽  
Mouse Mammary Gland ◽  
Immunohistochemical Localization ◽  
Oxide Synthase

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

mRNA expression of lipogenic genes in mouse mammary gland in different lactation stages

2012 ◽  
Vol 34 (3) ◽  
pp. 335-341
Author(s):  
Li-Qiang HAN ◽  
Hong-Ji LI ◽  
Yue-Ying WANG ◽  
Lin-Feng WANG ◽  
Guo-Qing YANG ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mrna Expression ◽  
Mouse Mammary Gland ◽  
Lipogenic Genes
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