Comparative evaluation of two ELISA kits for detecting antibodies to a          nonstructural protein of foot-and-mouth disease virus using serum samples collected from          naturally and experimentally infected cows

SummaryIsotype-specific antibody responses to foot-and-mouth disease virus (FMDV) were measured in the sera and upper respiratory tract secretions of vaccinated and susceptible cattle challenged with FMDV by direct contact or by intranasal inoculation. A comparison was made between cattle that eliminated FMDV and those that developed and maintained a persistent infection. Serological and mucosal antibody responses were detected in all animals after challenge. IgA and 1gM were detected before the development of IgG1and IgG2responses. 1gM was not detected in vaccinated cattle. Challenge with FMDV elicited a prolonged biphasic secretory antibody response in FMDV ‘carrier’ animals only. The response was detected as FMDVspecific IgA in both mucosal secretions and serum samples, which gained statistical significance (P< 0·05) by 5 weeks after challenge. This observation could represent the basis of a test to differentiate vaccinated and/or recovered convalescent cattle from FMDV ‘carriers’.

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Use of Slaughterhouses as Sentinel Points for Genomic Surveillance of Foot-and-Mouth Disease Virus in Southern Vietnam

Viruses ◽

10.3390/v13112203 ◽

2021 ◽

Vol 13 (11) ◽

pp. 2203

Author(s):

Umanga Gunasekara ◽

Miranda R. Bertram ◽

Do H. Dung ◽

Bui H. Hoang ◽

Nguyen T. Phuong ◽

...

Keyword(s):

Disease Virus ◽

Foot And Mouth Disease ◽

Principal Component ◽

Mouth Disease ◽

Serum Samples ◽

Southern Vietnam ◽

Serotype O ◽

Healthy Cattle ◽

Mouth Disease Virus ◽

Foot And Mouth

The genetic diversity of foot-and-mouth disease virus (FMDV) poses a challenge to the successful control of the disease, and it is important to identify the emergence of different strains in endemic settings. The objective of this study was to evaluate the sampling of clinically healthy livestock at slaughterhouses as a strategy for genomic FMDV surveillance. Serum samples (n = 11,875) and oropharyngeal fluid (OPF) samples (n = 5045) were collected from clinically healthy cattle and buffalo on farms in eight provinces in southern and northern Vietnam (2015–2019) to characterize viral diversity. Outbreak sequences were collected between 2009 and 2019. In two slaughterhouses in southern Vietnam, 1200 serum and OPF samples were collected from clinically healthy cattle and buffalo (2017 to 2019) as a pilot study on the use of slaughterhouses as sentinel points in surveillance. FMDV VP1 sequences were analyzed using discriminant principal component analysis and time-scaled phylodynamic trees. Six of seven serotype-O and -A clusters circulating in southern Vietnam between 2017–2019 were detected at least once in slaughterhouses, sometimes pre-dating outbreak sequences associated with the same cluster by 4–6 months. Routine sampling at slaughterhouses may provide a timely and efficient strategy for genomic surveillance to identify circulating and emerging FMDV strains.

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Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638720962070 ◽

2020 ◽

Vol 32 (6) ◽

pp. 933-937

Author(s):

Clare F. J. Browning ◽

Antonello Di Nardo ◽

Lissie Henry ◽

Tim Pollard ◽

Lynne Hendry ◽

...

Keyword(s):

Disease Virus ◽

Disease Surveillance ◽

Nonstructural Protein ◽

Foot And Mouth Disease ◽

Screen Test ◽

Mouth Disease ◽

Nonstructural Proteins ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Thermo Fisher Scientific

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

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