Getting a culture herpesviridae antigens first and second types is possible using cell cultures inoculated epithelial pig testicles and tracheal calf respectively. The incubation herpesviridae first and second types should be conducted on the above lines in cell culture incubator at a temperature of 37,5 °C for up to 10 days. To maximize the release of virus from cell culture fluid viral after incubation need three frozen at temperatures from –18 °C to + 20 °C. The resulting liquid is purified viral the culture by centrifugation. Determining the infectious activity of the culture liquid viral performed in response hemagglutination of horse erythrocytes suspension, and the material is titrated to 1: 128 in the two recurrence. Accounting reaction was performed at 2, 4, 6 and 8 hours. Infectious material volumetric activity was 1:4. Getting antigens envisages concentrating liquid viral culture fluid by reverse dialysis. To do this, conducted a study to identify the optimal concentration of antigen suitable for setting reaction diffusion precipitation. At 1:10 antigen concentration result of different reactions, depending on the account of the diffusion precipitation reactions. When concentration of EHV–1 antigen was found that the optimum dilution for its RDP is 1:20. In assessing the EHV–2 antigen, found that suitable for setting reaction diffusion precipitation RDP is an antigen concentrated from 1:60 to 1:20. In practical terms, most rational use of antigen, concentrated 20 times.Keeping culture antigens can be conducted frozen at minus 18 ° C, for 12 months because after six months of storage of the frozen infectious activity was not decreased. And in research in 12 months noted a line of precipitation in native samples and serum diluted 1:2. Working antigens for diffuse precipitation reaction must be sterile on various forms of bacteria and fungi. Therefore, samples of viral antigens were plated on agar culture media for general purpose (plain agar), after having spent preserving antigen using 0.01% solution mertiolyatu rate of 0.1 sm3/1sm3 culture fluid. Using such an environment can detect material in the test organisms belonging to different morphological groups. Research sterility subjected to viral antigens, herpesviridae infection on the first type of herpesviridae infection and the second type.
Detection of Antibody to Mycoplasma synoviae by Gel Diffusion Precipitation Reaction Using Commercially Available Antigen for Agglutination Reaction
