210 Effects of sciatic neurectomy on cortical bone microstructure in juvenile rats : Three-dimensional appearance of canal network evaluated by synchrotron radiation microtomography

The purpose of this study was to demonstrate the ability of computed microtomography based on monochromatic synchrotron radiation (SRμCT) in microstructural analysis of cortical bone. Tibial diaphyses of growing rats (14 wk, n = 8) undergoing unilateral sciatic neurectomy 8 wk before study were imaged with spatial volume resolution of 5.83 × 5.83 × 5.83 μm3 by SRμCT (20 keV) at the synchrotron radiation facility (SPring-8). Reconstructed image data were translated into local mineral densities by using a calibrated linear relationship between linear absorption coefficients and concentrations of homogeneous K2HPO4 solution. Pure bone three-dimensional images, produced by simple thresholding at a bone mineral density of 0.82 g/cm3, were analyzed for macro- and microscopic structural properties. In neurectomized hindlimbs, cortical canal network rarefaction as well as bone atrophy were found. The former was characterized by 30% smaller porosity, 11% smaller canal density in transverse section, and 38% smaller canal connectivity density than those in contralateral bone. On the other hand, no difference was found in bone mineral density between neurectomized and intact hindlimbs (1.37 vs. 1.36 g/cm3). In conclusion, SRμCT is a promising method for the three-dimensional analysis of cortical microstructure and the degree of mineralization in small animals.

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Time response of the canal network in cortical bone to sciatic neurectomy in growing rats: a synchrotron radiation μCT study

Journal of Biomechanics ◽

10.1016/s0021-9290(06)84833-5 ◽

2006 ◽

Vol 39 ◽

pp. S448

Author(s):

T. Matsumoto ◽

M. Yoshino ◽

K. Uesugi ◽

M. Tanaka

Keyword(s):

Synchrotron Radiation ◽

Cortical Bone ◽

Time Response ◽

Growing Rats ◽

Sciatic Neurectomy ◽

Canal Network

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615 Effects of Sciatic Neurectomy on Partial Cortical Canal Network of Tibiae in Growing Rats : Investigation Using Monochromatized Synchrotron Radiation Microtomography

The Proceedings of Conference of Kansai Branch ◽

10.1299/jsmekansai.2006.81._6-18_ ◽

2006 ◽

Vol 2006.81 (0) ◽

pp. _6-18_

Author(s):

Masayuki YOSHINO ◽

Takeshi MATSUMOTO ◽

Takahisa ASANO ◽

Hisashi NAITO ◽

Masao TANAKA

Keyword(s):

Synchrotron Radiation ◽

Growing Rats ◽

Sciatic Neurectomy ◽

Canal Network ◽

Synchrotron Radiation Microtomography

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Nondestructive imaging of the internal microstructure of vessels and nerve fibers in rat spinal cord using phase-contrast synchrotron radiation microtomography

Journal of Synchrotron Radiation ◽

10.1107/s1600577517000121 ◽

2017 ◽

Vol 24 (2) ◽

pp. 482-489 ◽

Cited By ~ 9

Author(s):

Jianzhong Hu ◽

Ping Li ◽

Xianzhen Yin ◽

Tianding Wu ◽

Yong Cao ◽

...

Keyword(s):

Spinal Cord ◽

Synchrotron Radiation ◽

Phase Contrast ◽

Three Dimensional ◽

Nerve Fibers ◽

The Body ◽

Rat Spinal Cord ◽

Nondestructive Imaging ◽

Synchrotron Radiation Microtomography ◽

The Brain

The spinal cord is the primary neurological link between the brain and other parts of the body, but unlike those of the brain, advances in spinal cord imaging have been challenged by the more complicated and inhomogeneous anatomy of the spine. Fortunately with the advancement of high technology, phase-contrast synchrotron radiation microtomography has become widespread in scientific research because of its ability to generate high-quality and high-resolution images. In this study, this method has been employed for nondestructive imaging of the internal microstructure of rat spinal cord. Furthermore, digital virtual slices based on phase-contrast synchrotron radiation were compared with conventional histological sections. The three-dimensional internal microstructure of the intramedullary arteries and nerve fibers was vividly detected within the same spinal cord specimen without the application of a stain or contrast agent or sectioning. With the aid of image post-processing, an optimization of vessel and nerve fiber images was obtained. The findings indicated that phase-contrast synchrotron radiation microtomography is unique in the field of three-dimensional imaging and sets novel standards for pathophysiological investigations in various neurovascular diseases.

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