scholarly journals The Relationship Between High Temperature Treatment during the Pupal Period and Male Sterility of the Silkworm, Bombyx mori L.

1977 ◽  
Vol 21 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Sadaya KATSUNO
Keyword(s):  
High Temperature ◽  
Male Sterility ◽  
Bombyx Mori ◽  
Temperature Treatment ◽  
High Temperature Treatment ◽  
Pupal Period ◽  
Bombyx Mori L ◽  
The Relationship ◽  
Silkworm Bombyx Mori

scholarly journals BmCncC/keap1-pathway is involved in high-temperature induced metamorphosis regulation of silkworm, Bombyx mori

2018 ◽  
Author(s):  
Jinxin Li ◽  
Tingting Mao ◽  
Zhengting Lu ◽  
Mengxue Li ◽  
Zhengting Lu ◽  
...  
Keyword(s):  
High Temperature ◽  
Bombyx Mori ◽  
Temperature Stress ◽  
Hormone Level ◽  
High Temperature Stress ◽  
Temperature Treatment ◽  
Endogenous Hormone ◽  
High Temperature Treatment ◽  
Regulation Mechanism ◽  
The Relationship

AbstractThe global warming has affected the growth, development and reproduction of insects. However, the molecular mechanism of high temperature stress-mediated metamorphosis regulation of lepidopteran insect has not been elucidated. In this study, the relationship between the insect developmental process and endogenous hormone level was investigated under high temperature (36 ° C) stress in Bombyx mori (B. mori). The results showed that the duration of 5th instar larvae were shortened by 28 ± 2 h, and the content of 20E was up-regulated significantly after 72 h of high temperature treatment, while the transcription levels of 20E response genes E93, Br-C, USP, E75 were up-regulated 1.35, 1.25, 1.28, and 1.27-fold, respectively. The high temperature treatment promoted the phosphorylation level of Akt and the downstream BmCncC/keap1 pathway was activated, the transcription levels of 20E synthesis-related genes cyp302a1, cyp306a1, cyp314a1 and cyp315a1 were up-regulated by 1.12, 1.51, 2.17 and 1.23-fold, respectively. After treatment with double stranded RNA of BmCncC (dsBmCncC) in BmN cells, the transcription levels of cyp302a1 and cyp306a1 were significantly decreased, whereas up-regulated by 2.15 and 1.31-fold, respectively, after treatment with CncC activator Curcumin. These results suggested that BmCncC/keap1-mediated P450 genes (cyp302a1, cyp306a1) expression resulted in the changes of endogenous hormone level, which played an important role in the regulation of metamorphosis under high temperature stress. Studies provide novel clues for understanding the CncC/keap1 pathway-mediated metamorphosis regulation mechanism in insects.Author SummaryMammalian nuclear transcription factor Nrf2 (NF-E2-related factor 2) plays an important role in the stress response of cells. CncC is a homolog of mammalian Nrf2 in insect, regulating the genes expression of insect antioxidant enzymes and cytochrome P450 detoxification enzyme. Evidence suggests that the CncC/Keap1 pathway also plays an important role in regulating insect development. Here, we investigated the regulatory mechanism between the CncC/Keap1 pathway and metabolism of silkworm hormones in Lepidoptera. We found that high temperature induction accelerated the development of silkworm, the ecdysone content and related metabolic genes in hemolymph were significantly up-regulated, the CncC/Keap1 pathway was activated, and the expression of BmCncC was significantly increased, indicating that the Cncc/Keap1 pathway plays an important role in this process. The expression of cyp302a1 and cyp306a1 was significantly decreased by RNA interference with BmCncC, which indicated that CncC in silkworm had a regulatory relationship with downstream 20E synthetic gene. In summary, the results indicate that the CncC/Keap1 pathway plays an important role in regulating hormone metabolism in silkworm, providing a basis for further study of the relationship between CncC/Keap1 pathway and development in insects.

Temperature and genotypic effects on the expression of pol cytoplasmic male sterility in summer rape

1991 ◽  
Vol 71 (3) ◽  
pp. 655-661 ◽  
Author(s):  
D. R. Burns ◽  
R. Scarth ◽  
P. B. E. McVetty
Keyword(s):  
High Temperature ◽  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Male Fertility ◽  
Inbred Lines ◽  
Temperature Treatment ◽  
High Temperature Treatment ◽  
Controlled Environment ◽  
Pollen Production

The effect of high temperature treatment on the expression of cytoplasmic male sterility (CMS) was studied using inbred lines of four summer rape (Brassica napus L.) cultivars. The nuclear genotypes of inbred lines from each of the cultivars Karat, Lergo, Marnoo and Regent were evaluated for their ability to maintain pollen sterility in the pol cytoplasm. Seven days' exposure to day/night temperatures of 30/24 °C in a controlled environment led to increased pollen production in all of the F1 populations (F1's) in the study. Maximum reversion to male fertility occurred 6–13 d after removal from the high temperature treatment. There was a significant linear relationship between daily mean temperatures in the field and mean male sterility index (MSI). In Lergo, Marnoo and Regent F1's, the effect of high temperatures on male sterility expression in terms of days to maximum pollen production in the field was accurately predicted by the values obtained in the controlled environment study. For the Karat F1's, the maximum response in the field occurred later than in the controlled environment. Variation in the stability of male sterility was evident in the F1's, indicating the presence of different maintainer genes among the inbred lines of each cultivar. The absence of environment-by-line interactions in the F1's of three cultivars indicated a high degree of stability for this trait. Selection for maintenance of high levels of male sterility may therefore be possible. Key words: Brassica napus, male fertility reversion

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