Purification and Identification of Antioxidant Peptides from Hydrolysates of Large Yellow Croaker (Pseudosciaena crocea) Scales

2020 ◽  
Vol 63 (2) ◽  
pp. 289-294
Author(s):  
Wenwei Chen ◽  
Yao Hong ◽  
Zhenbao Jia ◽  
Guangrong Huang
Keyword(s):  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Large Yellow Croaker ◽  
Pseudosciaena Crocea ◽  
Antioxidant Peptides ◽  
Peptide Hydrolysis ◽  
Good Source ◽  
Ionization Time ◽  
Molecular Weights ◽  
Flight Mass Spectrometry

HighlightsThree antioxidant peptides were efficiently obtained from large yellow croaker scales.The molecular weights of the three peptides were 879.4, 952.5, and 1070.4 Da, respectively.The large yellow croaker scales’ hydrolysates are a good source of natural antioxidants.Abstract. The scales of large yellow croaker () are typically discarded as waste during processing. In this study, three peptides with high antioxidant properties were purified from the scales via ultrafiltration and column chromatography. Through matrix-assisted laser desorption ionization/time-of-flight mass spectrometry, the sequences of the three peptides were identified as Gln-Arg-Pro-Pro-Glu-Pro-Arg (M1N4-1, 879.4 Da), Glu-Lys-Val-Trp-Lys-Tyr-Cys-Asp (M1N4-2, 1070.4 Da), and Val-Gly-Leu-Pro-Gly-Leu-Ser-Gly-Pro-Val-Gly (M2N2-1, 952.5 Da). The antioxidant activity of the peptides was directly determined through 1-diphenyl-2-picrylhydrazyl scavenging assay. Results suggested that large yellow croaker scales are a good source of natural antioxidants. Keywords: Antioxidant peptide, Hydrolysis, Pseudosciaena crocea, Scale.

DNA and BSA damage inhibitory activities, and anti-acetylcholinesterase, anti-porcine α-amylase and antioxidant properties of Dolichos lablab beans

2017 ◽  
Vol 8 (2) ◽  
pp. 881-887 ◽  
Author(s):  
Hosam M. Habib ◽  
Serah W. Theuri ◽  
Ehab Kheadr ◽  
Fedah E. Mohamed
Keyword(s):  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Good Source ◽  
Dolichos Lablab ◽  
Inhibitory Activities

The underutilized Kenyan variety of Dolichos lablab bean seeds serve as a good source of natural antioxidants, which can probably be effective in reducing the risk of occurrence of several diseases.

scholarly journals Self-Calibrated Warping for Mass Spectra Alignment

2011 ◽  
Vol 10 ◽  
pp. CIN.S6358 ◽  
Author(s):  
Q. Peter He ◽  
Jin Wang ◽  
James A. Mobley ◽  
Joshua Richman ◽  
William E. Grizzle
Keyword(s):  
Mass Spectrometry ◽  
Biological Entity ◽  
Data Set ◽  
Ionization Time ◽  
Molecular Weights ◽  
Data Alignment ◽  
Flight Mass Spectrometry ◽  
Alignment Algorithms ◽  
Wide Range ◽  
Surface Enhanced

With recent advances in mass spectrometry (MS) technologies, it is now possible to study protein profiles over a wide range of molecular weights in small biological specimens. However, MS spectra are usually not aligned or synchronized between samples. To ensure the consistency of the subsequent analysis, spectrum alignment is necessary to align the spectra such that the same biological entity would show up at the same m/z value for different samples. Although a variety of alignment algorithms have been proposed in the past, most of them are developed based on chromatographic data and do not address some of the unique characteristics of the serum or other body fluid MS data. In this work, we propose a self-calibrated warping (SCW) algorithm to address some of the challenges associated with serum MS data alignment. In addition, we compare the proposed algorithm with five existing representative alignment methods using a clinical surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) data set.

scholarly journals Physico-chemical and antioxidant properties of barberry juice powder and its effervescent tablets

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Sara Naji-Tabasi ◽  
Bahareh Emadzadeh ◽  
Mostafa Shahidi-Noghabi ◽  
Mohammadreza Abbaspour ◽  
Ehsan Akbari
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Phenol Content ◽  
Good Source ◽  
Disintegration Time ◽  
Physico Chemical ◽  
High Antioxidant Activity ◽  
Spray Dried

Abstract Background Barberry fruit is a good source of natural antioxidants and various functional compounds. Different concentrations of maltodextrin (10, 13, and 16% w/w) were used to produce spray-dried barberry juice powder and the powders (50, 60, and 70%) were applied to create effervescent tablets. Results The results showed that by increasing the amount of maltodextrin concentration, moisture, and water activity decreased (p < 0.05), but antioxidant activity increased. The barberry powder prepared with 13% (w/w) maltodextrin showed appropriate flowability, color, high antioxidant activity, and phenol content. The presence of high amounts of barberry powder in the tablet increased the disintegration time (1.02–4.03 min). Conclusions The tablet containing 60% barberry powder was selected as the best sample. Based on the results, barberry tablets with good color, high antioxidant activity, and phenolic compounds can be used as a ‘ready-to-drink’ product.

Sign in / Sign up

Export Citation Format

Share Document