Characterization of Effect of Gamma Ray Induced Mutations on Morpho-Agronomic Traits of Dolichos Lablab (Lablab purpureus l.) Sweet

Lablab purpureus (L.) Synonym Dolichos lablab (L.) Sweet; is a minor and an important multipurpose legume. Despite this it classified as an orphaned crop hence few studies on its improvement have been undertaken. Limited studies have been carried out on use of mutation on dolichos improvement. Four elite Lablab genotypes coded G1, B1, M5 and W7 were mutagenized with two doses of gamma radiation 300Gy and 400 Gy with objective to study the effect of mutation on various agronomical traits at M2 generation. Data on effect of irradiation on lablab was collected 10 randomly selected plants of on each plot .The traits evaluated were leaf length, leaf width, number of raceme per plant, raceme length, pod length, pod width, plant height, dry seed yield per plant and seed length and seed width. For these traits mean of mutant plants was significantly different from mean of control plants at P≤0.05. Mutant accessions of B1, M5 and W7 genotypes exhibited wide mutation spectra after mutagenesis of albino, leafiness, upright single stem, seedless pod, short dwarf pod, variegated leaf, variation in flower colour, variation in growth habit, leaf shape, seed colour. Gamma irradiation doses of 300 Gy and 400 Gy were potent in mutagenesis of the studied dolichos lablab. The agronomically diverse mutants are an important resource for dolichos improvement through breeding and genetic studies. Mutants with variegated leafs and flower colour can be used in the landscaping while those different seed colour could be used in the improvement of sensory and cooking qualities of other native dolichos lablab beans.

Biology and non-chemical management of Spermacoce verticillata and Spermacoce densiflora

The weed species Spermacoce densiflora DC. and Spermacoce verticillata L. have shown an increase in their occurrence in cultivated areas in the northeast region of Brazil, and field observations have reported their ineffective control with chemicals. This study aimed to evaluate the germination of S. densiflora and S. verticillata under constant (15, 20, 25, 30 and 35°C) and alternating temperatures (20–30°C) in dark and in constant light; the emergence of seedlings from seven sowing depths (0, 0.5, 1, 2, 4, 6 and 10 cm); the emergence of seedlings under five types of cover crop straw (Crotalaria juncea L., Pennisetum glaucum (L.) R. Br., Sorghum bicolor (L.) Moench, Dolichos lablab L., and Cajanus cajan L.) and four amounts (nil and the average, half and twice the amount of straw produced in the field); and germination at different aqueous cover crop extract concentrations (0, 20, 40, 60 and 100%) to measure allelopathic potential of cover crop straw. The results showed that S. densiflora has positive photoblastic behavior. The alternating temperature provided the highest percentage of germination and germination speed index (GSI) for both species. S. densiflora and S. verticillata seedling emergence decreased with an increase in depth, with no germination at a depth of 10 cm. The presence of straw impaired the emergence of seedlings of S. densiflora and S. verticillata by delaying and even preventing germination from occurring. The gradual increase in the aqueous extract concentrations was accompanied by lower percentages of germination and GSI for S. densiflora and S. verticillata.

Evaluation of Proximate and Mineral Composition of Mutant Dolichos Lablab (Lablab purpureus L.) Accessions in Kenya

Plant breeding through induced mutation technology is a potent method to creating new variants of food crops with of desirable phenotypic, genetic and biochemical functions. It is a catalyst in developing improved crop varieties where classical hybridization or selection have limitations. It has been used to improve nutrition quality and higher yield in a number of legumes. Dolichos Lablab (Lablab purpureus L) is multipurpose legume that has not been exploited extensively for food nutritional properties through breeding. The purpose of the study was, therefore, to generate awareness that nutritional status of D. Lablab could be improved through mutation induction and be a good source of food components essential for good health. Twenty-four dolichos Lablab germplasms including 20 mutant accessions and 4 commercial genotypes were evaluated for proximate values and mineral contents in Kenya in 2021 based on Association of Official Analytical Chemists (AOAC). Data analysis was based on least significant difference (P = .05).The revealed percent moisture, ash crude fat, crude fibre, crude proteins and total starch content ranged : 7.35% -11.84%, 1.09 -2.90%, 3.35% - 8.05% , 8.86 - 12.70% , 20.03% - 28.87% and 25.50% - 39.00% .The mineral content of phosphorous (P), potassium(K) and calcium (Ca) ranged from 27mg - 57mg, 132mg -297mg and 7mg - 19mg. A significant positive correlation between ash levels to P and K concentration and on P to K concentration. The result of analysis ranked 9 (WT026, WT018 ,MT110 , BT188 , BT032, BT114, MT076 BT137,GT09) different accessions that positively contributed to the nutritional content of the investigated dolichos lablab accessions. Further research on the superior accessions can be done on yield potential, resistance to biotic and abiotic constrains, sensory preferences or used in bio fortification of existing genotypes.

Performance and Efficiency of Azadirachta Indica, Guazuma Ulmifolia and Dolichos Lablab in Municipal Waste Diagnosis Process

The experiment was carried out at various dosages of the crude water extract by natural dry seeds. Using a jar test experiment, the experiment was carried out at various dosages of the crude water extract by dry seeds of Azadirachta indica, Guazuma ulmifolia, and Dolichos lablab. The optimum dosage of the coagulant was determined. Quality parameters of the waste waters were measured before and after treatment to assess the removal efficiency of major pollutants of concern in waste water treatment, such as suspended solids, COD, BOD, pH, TDS, Sulphates, and Chlorides. Azadirachta indica was found to be the most suitable natural coagulant for municipal waste water treatment when compared to Guazuma ulmifolia and Dolichos lablab. The use of a locally available natural coagulant for water treatment was found to be suitable, easier, cost effective, and environmentally friendly.

Simple Sequence Repeats Marker based Detection of Genetic Diversity of Indian Bean Dolichos lablab (L.) of Family Fabaceae

Background: Assessment and molecular characterization of genetic diversity among the Dolichos lablab (L.) have huge implication in scheming strategies for breeding. In India, there is less comprehensive information on the potential genetic diversity of lablab bean and this is a major challenge for systematic use of lablab bean in genetic breeding programs. To exploit the available trait of interest, the genetic diversity of the locally available genotypes must be known. The PCR-based SSR approach requires just nanogram amounts of template DNA, subjected to quick detection and less influenced by environment. In this manner SSR have been utilizing broadly to study hereditary assorted qualities of yields.Methods: In this study, genetic diversity on ten Indian Dolichos lablab (L.) genotypes were surveyed utilizing SSR markers at Department of Botany, Bundelkhand University, Jhansi during 2019-20 got from Indian gene bank. For SSR analysis, 5 primers were used for checking the diversity in Dolichos lablab (L.) and the performance of markers were measured by using 4 parameters (1) PIC, (2) Mean resolving Power, (3) Resolving Power and (4) MI. Result: SSR (05) markers produced a total of 51 bands, out of which 45 bands (88.23%) were polymorphic which varies from 6 (Primer-111) to 15 (Primer-AGB-9) with mean of 10.5 bands per primer. The polymorphic information content value ranges from 0.265 to 0.488 with a mean value of 0.390. The value of Matrix index is 3.2. The value of resolving power ranges from 2.2 to 12.60 with a mean value of 7.40. In the UPGMA dendogram, the 10 genotypes were separated into two main clusters with all the primers used. Our present examination uncovered that genetic markers might be effectively used for deciding hereditary variety and connections in Dolichos lablab (L.) genotypes and could be utilized as a part of breeding programs.

Antimicrobial activity of a crude peptide extract from lablab bean (Dolichos lablab) for semi-dried rice noodles shelf-life

This study provides an application of new, natural source of crude peptide extract from Lablab bean (CPL). Use of additive such as benzoate in the rice noodle industry is a common practice and has several bene?cial effects on quality and shelf-life. However, the shelf-life of semi-dried rice noodles can be extended by crude peptide extract with acceptable quality when compared to other additives. This research aimed to extract CPL and determine its effect on the growth of microorganisms. The use of the CPL to extend the shelf-life of semi-dried rice noo-dles was compared with other natural extracts (chitosan and thymol) and benzoic acid. The CPL samples were extracted using 5% pepsin and incubated for up to 24 h. CPL at 200 mg/mL could be used as the growth inhibitor for Bacillus cereus but not for Staphylococcus aureus and Escherichia coli. It was found that noodles with CPL had the highest cooking loss (4.69) and lowest tensile strength (22.6 g.force). Overall-liking scores showed slightly liked (6.0 out of 9) and 68% of the consumers accepted the CPL-treated noodles. Shelf-life testing showed that CPL could extend the shelf-life of the noodles for 3 days than the control (1 day). Using 200 mg/mL of CPL could extend the shelf-life more than 3 times when compared to the control noodle (no additive). The CPL was nearly as effective as benzoic acid that could be kept for 3 days. Hedonic score in overall-liking showed a slightly like (6.2) for the noodles with CPL. This study suggests the application of adding CPL could be used as new natural additive and seems to be promising to apply in many food products such as pasta or sausages.

Seasonal dynamics of bean aphids and its relationship with the abundance of Lady Bird Beetles

The seasonal dynamics of aphid Aphis craccivora Koch (Hemiptera: Aphididae) on four bean species namely country bean Lablab purpureus, yard long bean Vigna sesquipedalis, hyacinth bean Dolichos lablab and bush bean Phaseolus vulgaris were studied from September to December 2017 in the experimental field of the Department of Entomology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh. The study also investigated the relationship between the abundance of aphid and its predatory insect lady bird beetle. Aphid abundance on the bean species showed increasing trend from the 4th week of September and reached to the peak during the 2nd week of November and then declined. Bush bean and yard long bean, respectively depicted the higher and lower abundances of aphid throughout the study. Relationship between the abundance of aphid and lady bird beetle on the bean species were positively correlated. Aphid showed negatively correlation with temperature and rainfall, and positive correlation with relative humidity, and the correlations were not significant. Multiple regression equation showed that temperature had the highest effect which contributed 16.1 - 19.2% role on the population of aphid. Bangladesh J. Zool. 48(2): 357-363, 2020