Molecular characterization of eight horse breeds in Algeria using microsatellite markers

Abstract. Amine BME, Naima B, Mohammed B, Abdelkader AA, Youssouf HH, Mohammed P, Suheill GSB. 2020. Molecular characterization of eight horse breeds in Algeria using microsatellite markers. Biodiversitas 21: 4107-4115. The Algerian horse population Bar, Origin Barb unknown, Arabian-Barb, Arabian, Thoroughbred (Pur sang); French Trotter, Algerian saddle, and Saddle are under a significant loss of genetic variability. This should be evaluated before any breed conservation and management program. For this purpose, genetic analyzes of diversity and differentiation were carried out on eight breeds horses reared in Algeria with 17 microsatellites. All microsatellite markers were highly polymorphic in all Breeds. A total of 170 alleles from 17 microsatellite loci were detected in 279 horses. The average number of alleles per locus was highest in Arabian-Barb horses (7.88) and lowest in Saddle breed (4.94). The observed heterozygosity interbreeds ranged from 0.685 to 0.625 for Barb and Saddle, while the expected heterozygosity was ranged from 0.746 to 0.678 for Arabian-Barb and Algerian Saddle respectively, the Ho value is lower than that of He value. Genetic diversity between breeds was significant, based on Fst. Three different approaches to assess genetic relationships have been applied; namely, genetic distances, factorial correspondence analysis, and admixture analysis have shown that a significant amount of genetic variation is maintained in populations of native horses and other breeds: the Barb, Arabian-Barb, Barb (Oi). Algerian Saddle and Saddle breeds appear to be the most genetically related and support that there are crosses between these two breeds.

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Transferability and Characterization of Microsatellite Markers from Byrsonima Cydoniifolia A. Juss. (MALPIGHIACEAE) in Seven Related Taxa from Cerrado Biome Reveal Genetic Relationships

10.21203/rs.3.rs-238340/v1 ◽

2021 ◽

Author(s):

Vanessa Bernardes ◽

Devanir M. Murakami ◽

Nair Bizão ◽

Tamara N. Souza ◽

Marcos J. da Silva ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Genetic Divergence ◽

High Efficiency ◽

Population Studies ◽

Genetic Relationships ◽

Species Occurrence ◽

Genetic Population ◽

Expected Heterozygosity

Abstract Byrsonima Rich. is one of the largest genera of the Malpighiaceae family, with 97 species occurrence in Brazil. In this study, 17 microsatellite markers previously developed and characterized in Byrsonima cydoniifolia A. Juss. were tested for seven related taxa. All species tested here are native to Brazil, and of these species four are endemic. Cross-amplification as successfully optimized with high efficiency for all species. Microsatellite markers panels ranged from 11 (64,8%) transfered markers in B. viminifolia to 6 (35.2%) in B. umbellata. All loci were genotyped for 16 individuals of each species, except for B. viminifolia (14 individuals), then the polymorphic loci were characterized. The total number of alleles per locus across tested species ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (Ho = 0.312; He = 0.436) and B. subterranea presented the highest values (Ho = 0.687; He = 0.778). A greater number of microsatellite markers should be developed for B. umbellata, once that the markers set transferred is reasonably informative, due to the greatest genetic divergence between species. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91x10− 6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.

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Genetic diversity in table grapes based on RAPD and microsatellite markers

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2011000900010 ◽

2011 ◽

Vol 46 (9) ◽

pp. 1035-1044 ◽

Cited By ~ 9

Author(s):

Patrícia Coelho de Souza Leão ◽

Sérgio Yoshimitsu Motoike

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Genetic Relationships ◽

Similarity Index ◽

Genetic Distances ◽

Table Grape ◽

Group Method ◽

Molecular Characteristics ◽

Table Grapes ◽

Pair Group

The objective of this work was to analyze the genetic diversity of 47 table grape accessions, from the grapevine germplasm bank of Embrapa Semiárido, using 20 RAPD and seven microsatellite markers. Genetic distances between pairs of accessions were obtained based on Jaccard's similarity index for RAPD data and on the arithmetic complement of the weighted index for microsatellite data. The groups were formed according to the Tocher's cluster analysis and to the unweighted pair‑group method with arithmetic mean (UPGMA). The microsatellite markers were more efficient than the RAPD ones in the identification of genetic relationships. Information on the genetic distance, based on molecular characteristics and coupled with the cultivar agronomic performance, allowed for the recommendation of parents for crossings, in order to obtain superior hybrids in segregating populations for the table grape breeding program of Embrapa Semiárido.

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Molecular Characterization of Common Bean (Phaseolus vulgaris L.) Genotypes Using Microsatellite Markers

Journal of Advances in Biology & Biotechnology ◽

10.9734/jabb/2017/33519 ◽

2017 ◽

Vol 13 (2) ◽

pp. 1-15 ◽

Cited By ~ 2

Author(s):

Pam Gyang ◽

Evans Nyaboga ◽

Edward Muge

Keyword(s):

Phaseolus Vulgaris ◽

Microsatellite Markers ◽

Common Bean ◽

Molecular Characterization ◽

Phaseolus Vulgaris L

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Ampelographic and molecular diversity among grapevine (Vitis spp.) cultivars

Czech Journal of Genetics and Plant Breeding ◽

10.17221/72/2008-cjgpb ◽

2009 ◽

Vol 45 (No. 4) ◽

pp. 160-168 ◽

Cited By ~ 12

Author(s):

A. Sabir ◽

S. Tangolar ◽

S. Buyukalaca ◽

S. Kafkas

Keyword(s):

Molecular Characterization ◽

Molecular Diversity ◽

Genetic Distances ◽

Mean Values ◽

Issr Analysis ◽

Geographic Origins ◽

The Mean ◽

Vitis Spp

This study presents the ampelographic and molecular characterization of 44 grapevine cultivars. Ampelographic data were obtained during two vegetation periods using the latest version of the descriptors. Based on the mean values transformed by the method indicated in IBPGR publications, a dendrogram was constructed. ISSR analysis was also employed to characterize the genotypes at the DNA level. Twenty primers, selected on the basis of their discriminating potential, generated a total of 157 bands, of which 140 were polymorphic. The dendrograms constructed by the two approaches were largely similar in both the clustering position and divergence of varietal groups. The least distance was observed between Yuvarlak Cekirdeksiz and Superior Seedless. The clustering position of cultivars throughout the dendrograms was basically related to the genetic distances and main uses, as well as to geographic origins.

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Microsatellite markers for identification of a group of italian olive accessions

Scientia Agricola ◽

10.1590/s0103-90162009000500014 ◽

2009 ◽

Vol 66 (5) ◽

pp. 685-690 ◽

Cited By ~ 15

Author(s):

Innocenzo Muzzalupo ◽

Francesca Stefanizzi ◽

Amelia Salimonti ◽

Rosanna Falabella ◽

Enzo Perri

Keyword(s):

Microsatellite Markers ◽

Molecular Characterization ◽

Ssr Markers ◽

Olea Europaea ◽

Fruit Trees ◽

Ssr Primers ◽

Olive Cultivars ◽

Olea Europaéa

Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.

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