scholarly journals Ampelographic and molecular diversity among grapevine (Vitis spp.) cultivars

2009 ◽  
Vol 45 (No. 4) ◽  
pp. 160-168 ◽  
Author(s):  
A. Sabir ◽  
S. Tangolar ◽  
S. Buyukalaca ◽  
S. Kafkas
Keyword(s):  
Molecular Characterization ◽  
Molecular Diversity ◽  
Genetic Distances ◽  
Mean Values ◽  
Issr Analysis ◽  
Geographic Origins ◽  
The Mean ◽  
Vitis Spp

This study presents the ampelographic and molecular characterization of 44 grapevine cultivars. Ampelographic data were obtained during two vegetation periods using the latest version of the descriptors. Based on the mean values transformed by the method indicated in IBPGR publications, a dendrogram was constructed. ISSR analysis was also employed to characterize the genotypes at the DNA level. Twenty primers, selected on the basis of their discriminating potential, generated a total of 157 bands, of which 140 were polymorphic. The dendrograms constructed by the two approaches were largely similar in both the clustering position and divergence of varietal groups. The least distance was observed between Yuvarlak Cekirdeksiz and Superior Seedless. The clustering position of cultivars throughout the dendrograms was basically related to the genetic distances and main uses, as well as to geographic origins.

Prevalence and Molecular Characterization of Microbial Contaminants in Raw Cow Milk of Tangail District in Bangladesh

2021 ◽  
Vol 17 ◽  
Author(s):  
Shahin Mahmud ◽  
Md. Firoz Ali ◽  
Md. Omar Faruque ◽  
Muhammad Wasim ◽  
Fatematuz Zuhura Evamoni ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Raw Milk ◽  
Health Impact ◽  
Cow Milk ◽  
Milk Consumption ◽  
Salmonella Spp ◽  
Mean Values ◽  
The Mean ◽  
Quality Of Milk

Objectives: The aim of this study is to evaluate the microbial quality and associated health Methodology: An intensive study was carried out from January¬ to October 2019 at the Tangail district of Bangladesh to assess bacterial contamination of raw milk. A total of 60 samples were examined by following the standard bacteriological methods and the health impact was evaluated with the help of a semi-structured-based questionnaire. Molecular characterization of isolated bacteria was carried out by PCR. Results: Results revealed the mean values of TVC and TCC in raw milk, udder, oil, and utensil samples were 7.6×106, 8.8×106, 4.6×106, and 1.3×106 cfu/ml and 3.7×105, 1.4×106, 4.4×105, and 8.9×104 cfu/ml, respectively. The yeast and mold in raw milk, udder, and oil were 1.5×103, 1.8×103, and 1.3×102cfu/ml, respectively, and the mean values of E. coli in the above-mentioned samples were 1.9×103, 1.8×104, 2.1×103, and 1.6×103cfu/ml, respectively. On the other hand, the mean values of Salmonella spp. in raw milk, udder, oil, and utensil samples were 2.4×102, 7.9×102, 1.5×102, and 1.1×102cfu/ml, respectively. Some selected isolates confirmed by molecular identification were tested for their sensitivity against some common antibiotics used in Bangladesh. Escherichia coli showed 70% resistance to Amoxicillin and 90% sensitivity to Ciprofloxacin whereas Salmonella spp. And Staphylococcus aureus showed 90% resistance to Ampicillin and both were 80% and 90% sensitive to Ciprofloxacin, respectively. Conclusion, Significance, and Impact of Study: Milk producers should be appropriately trained to monitor the overall hygienic conditions surrounding the production and handling of milk until it reaches the consumer. Frequent monitoring of the milk production facility, occasional testing of raw milk, and minimal use of antibiotics will ensure the quality of milk. Consumption of quality milk and avoidance of raw milk consumption will reduce the health risk of the consumers.

scholarly journals Aerobic Plate Count of Contaminants and Molecular Characterization of Eschereichia Coli in Raw Chicken Meat in Ismailia, Egypt

2020 ◽  
Vol 2 (2) ◽  
pp. 23-30
Author(s):  
El-Sayed A. Afify ◽  
Fahim A Shaltout ◽  
Zakaria, I. M
Keyword(s):  
Molecular Characterization ◽  
Sensitivity And Specificity ◽  
Foodborne Pathogens ◽  
Broiler Chicken ◽  
Plate Count ◽  
Chicken Breast ◽  
Mean Values ◽  
The Mean ◽  
Aerobic Plate Count

A total number of 100 samples from ten random broiler chicken carcasses (breast and thigh) were collected from an automatic poultry slaughtering plant in Ismailia city, Egypt. The mean values of Enterobacteriacae count were 5.9x104±9.7x103 cfu/g and 7.1x 104 ± 1.1x104 cfu/g for chicken breast and thigh samples respectively. The prevalence of E.coli were 12% and 9% breast and thigh samples examined, respectively. They are serologically identified as 33.35 and 22.2% O157:H7 (EHEC) , 16.6% and 11.1% O114:H21(EPEC), 16.6% and 33.3 %O127:H6 (ETEC) , 0% and 0% O126 (ETEC) and 33.3% and 0% O26 (EHEC) for breast and thigh samples, respectively. The incidence of E.coli O157:H7 was 100% in both serological and PCR methods from biochemical positive E.coli samples. Culture is specific and cheap whereas PCR is sensitive and expensive, hence, we recommend both culture and molecular methods, which improve sensitivity and specificity, to enhance detection of foodborne pathogens including E.coli.

The Service Time Properties of an Unreliable Server Characterize the Exponential Distribution

1994 ◽  
Vol 26 (01) ◽  
pp. 172-182 ◽  
Author(s):  
Z. Khalil ◽  
B. Dimitrov
Keyword(s):  
Exponential Distribution ◽  
Service Time ◽  
Initial Conditions ◽  
Mean Values ◽  
Unreliable Server ◽  
Service Disciplines ◽  
The Mean ◽  
Server Reliability ◽  
Preemptive Repeat Different

Consider the total service time of a job on an unreliable server under preemptive-repeat-different and preemptive-resume service disciplines. With identical initial conditions, for both cases, we notice that the distributions of the total service time under these two disciplines coincide, when the original service time (without interruptions due to server failures) is exponential and independent of the server reliability. We show that this fact under varying server reliability is a characterization of the exponential distribution. Further we show, under the same initial conditions, that the coincidence of the mean values also leads to the same characterization.

scholarly journals A METHODOLOGICAL APPROACH TO THE CHARACTERIZATION OF BRAIN GLIOMAS, BY MEANS OF SEMI-AUTOMATIC MORPHOMETRIC ANALYSIS

2014 ◽  
Vol 33 (3) ◽  
pp. 201 ◽  
Author(s):  
Artur Dawid Surowka ◽  
Dariusz Adamek ◽  
Edyta Radwanska ◽  
Marek Lankosz ◽  
Magdalena Szczerbowska-Boruchowska
Keyword(s):  
Aspect Ratio ◽  
Digital Image Analysis ◽  
Methodological Approach ◽  
Morphometric Parameters ◽  
Mean Values ◽  
The Mean ◽  
Analytical Tools ◽  
Brain Gliomas

The aims of this paper were to present a reliable morphometric procedure for glioma analysis for preliminary prognosis and to develop a semi-automatic procedure that is easy to use. The data presented are important to the extent that they verify the reliability of the results by showing that they are consistent with the findings from more complicated automatic analytical tools. The objects for analysis were digital images of haematoxylin-eosin stained glioma samples. The overall analysis consisted of digital image analysis and the determination of morphometric parameters. Interestingly, an increase in the mean values of aspect ratio with increasing malignancy grade was found. Moreover, the morphometric parameters in relation to the histological origin of gliomas were examined and it was found that, the cellular nuclei of glioblastoma multiforme reveal the biggest mean values of aspect ratio compared with other gliomas.

scholarly journals Biomechanical characterization of swimmers with physical disabilities

Motricidade ◽  
2018 ◽  
Vol 14 (4) ◽  
pp. 103-112 ◽  
Author(s):  
Valdir Junior ◽  
Alexandre Medeiros ◽  
Kelly Jesus ◽  
Nuno Domingos Garrido ◽  
Rui Corredeira ◽  
...  
Keyword(s):  
Swimming Performance ◽  
Velocity Variation ◽  
Swimming Velocity ◽  
Stroke Length ◽  
Stroke Frequency ◽  
Mean Values ◽  
Functional Classes ◽  
The Mean ◽  
Incremental Protocol

The evaluation of swimming technique is one of the main aspects to be considered in any training program, with biomechanics being an important source of knowledge. It was our objective to characterize the biomechanical parameters (SL and SF) relating them to the swimming velocity (v) at different intensities and to analyze within each swimming stroke cycle the intra-cyclic velocity variation (IVV) in a group of motor disabled swimmers. Eight disabled male swimmers (25.83 ± 2.93 years old, 72.45 ± 9.26 kg body mass and 1.79 ± 0.11 m of height) of the following functional classes: S6 (n = 1), S8 (n = 2) and S9 (n = 5) participated in this study. Swimmers were evaluated in the kinematic parameters v, stroke frequency (SF) and stroke length (SL) along with an incremental protocol of 6 x 200 m in the the crawl stroke. Data were registered in each step at the distances of 100 and 175 m. With increasing velocity, the mean values of SL decreased while the mean values of SF increased. To achieve higher swimming velocities, swimmers compensated the lack of the propulsive segment increasing SF to increase swimming speed. For the mean values of IVV at 100m distance, a decrease between the first and second levels, followed by a tendency to stabilize from the 2nd to the 6th level is presented. For the 175 m distance, there was a decrease in IVV with an increase in swimming velocity. Stroke frequency is directly related to the magnitude of IVV, which directly influences swimming performance.

scholarly journals Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA

2011 ◽  
Vol 56 (No. 4) ◽  
pp. 163-175 ◽  
Author(s):  
L. Vostrý ◽  
O. Kracíková ◽  
B. Hofmanová ◽  
V. Czerneková ◽  
T. Kott ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Analysis ◽  
Genetic Difference ◽  
Principal Component ◽  
Genetic Distances ◽  
Mean Value ◽  
Pedigree Information ◽  
Mean Values ◽  
The Mean ◽  
Coefficient Of Relationship

The Old Kladruber horse is the oldest original Czech horse breed &ndash; gene resource, with pedigree records spanning three centuries. Because the population is closed, there is a concern about the loss of genetic variation. The genetic diversity within and amongst sire lines was evaluated using microsatellite markers and based on pedigree information. The DNA analysis, covering 16 microsatellite loci, revealed that the genetic diversity (observed heterozygosity = 0.637, genetic diversity = 0.678 and the mean number of alleles = 8.25) for the Old Kladruber horse is consistent with other populations of horses. Inter-line genetic difference in sire lines reached the mean value (the F<sub>ST</sub> values ranged from 0.020 to 0.017). Differences amongst the sire lines were identified using genetic distances and principal component analysis. One gene cluster comprised black variation sire lines, while the second cluster included the sire lines of the grey variation. In the subjects monitored, the average inbreeding coefficient of 0.076 was estimated on the basis of pedigree information. The highest mean values of the coefficient of relationship were estimated within the colour variations. When comparing the genetic distance and the average coefficient of relationship, differences were found out. These results are useful for the development of breeding strategies which consider classical horse breeding as well as recent achievements of population and conservation genetics.

Molecular characterization of the Rpv3 locus towards the development of KASP markers for downy mildew resistance in grapevine (Vitis spp.)

Euphytica ◽  
2021 ◽  
Vol 218 (1) ◽  
Author(s):  
Andriele Wairich ◽  
Jaiana Malabarba ◽  
Vanessa Buffon ◽  
Diogo D. Porto ◽  
Roberto Togawa ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Molecular Characterization ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Vitis Spp ◽  
Kasp Markers

scholarly journals Characterization of Phytophthora capsici Isolates from Processing Pumpkin in Illinois

Plant Disease ◽  
2005 ◽  
Vol 89 (2) ◽  
pp. 191-197 ◽  
Author(s):  
S. Z. Islam ◽  
M. Babadoost ◽  
K. N. Lambert ◽  
A. Ndeme ◽  
H. M. Fouly
Keyword(s):  
Mating Type ◽  
Random Amplified Polymorphic Dna ◽  
Phytophthora Capsici ◽  
Group Analysis ◽  
Genetic Distances ◽  
Growth Patterns ◽  
Pair Group ◽  
Genetic Groups ◽  
The Mean

This study was conducted to investigate pathogenic, morphologic, and genetic variations among Phytophthora capsici isolates from processing pumpkin (Cucurbita moschata) fields in Illinois. Random amplified polymorphic DNA (RAPD) markers were employed to assess genetic variation among 24 isolates of P. capsici from 10 individual fields at six locations. Unweighted mean pair group analysis clustered isolates into six groups. The genetic distances ranged from 0.03 to 0.45. Inoculation of pumpkin seedlings in the greenhouse revealed that the isolates belonged to six distinct genetic groups differing significantly (P = 0.05) in virulence. Isolates tested exhibited four growth patterns in culture: cottony, rosaceous, petaloid, and stellate. P. capsici isolates, including an ATCC isolate (ATCC-15427), with cottony growth pattern did not grow at 36°C. The mean oospore diameter of A1 mating type isolates was greater than that of A2 mating type isolates. Nine of 24 isolates tested produced chlamydospores in V8-CaCO3 liquid medium.

scholarly journals Molecular Characterization of Salmonella from Human and Animal Origins in Uganda

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Atek Atwiine Kagirita ◽  
Andrew Baguma ◽  
Tonny Jimmy Owalla ◽  
Joel Bazira ◽  
Samuel Majalija
Keyword(s):  
Multiplex Pcr ◽  
Molecular Characterization ◽  
Molecular Diversity ◽  
One Health ◽  
Virulence Genes ◽  
Classification Scheme ◽  
Virulence Gene ◽  
Clinical Presentations ◽  
Animal Isolates

Sporadic Salmonella outbreaks with varying clinical presentations have been on the rise in various parts of Uganda. The sources of outbreaks and factors underlying the different clinical manifestation are curtailed by paucity of information on Salmonella genotypes and the associated virulence genes. This study reports molecular diversity of Salmonella enterica and their genetic virulence profiles among human and animal isolates. Characterization was done using Kauffman-White classification scheme and virulence genes analysis using multiplex PCR. Overall, 52% of the isolates belonged to serogroup D, 16% to serogroup E, 15% to poly F, H-S, and 12% to serogroup B. Serogroups A, C1, and C2 each consisted of only one isolate representing 5%. Virulence genes located on SPI-1 [spaN and sipB] and on SPI-2 [spiA] in addition to pagC and msgA were equally distributed in isolates obtained from all sources. Plasmid encoded virulence gene spvB was found in <5% of isolates from both human epidemic and animal origins whereas it occurred in 80% of clinical isolates. This study reveals that serogroup D is the predominant Salmonella serogroup in circulation and it is widely shared among animals and humans and calls for joint and coordinated surveillance for one health implementation in Uganda.

scholarly journals EVALUATION OF RADIOACTIVITY IN THE PHOSPHOGYPSUM STOCKPILE OF “HIV” VELES, THE REPUBLIC OF NORTH MACEDONIA

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Mitko Janchev ◽  
Ivan Boev ◽  
Zdenka Stojanovska ◽  
Blazo Boev
Keyword(s):  
Natural Radionuclides ◽  
Radiation Risk ◽  
Waste Product ◽  
Gross Alpha ◽  
Mean Values ◽  
The Mean ◽  
Specific Activities ◽  
The Republic ◽  
High Concentrations

The production of phosphoric acid from natural phosphate ore generates an industrial waste product named phosphogypsum. Phosphogypsum contains considerable amounts of natural radionuclides from the 238U chain, originating from the ore but enriched during the technological process. In order to perform radiological characterization of the “HIV” (Chemical Industry Veles) phosphogypsum stockpile, five phosphogypsum samples were collected and analyzed. The mean values of gross alpha and beta specific activities ± standard deviation values were: (950±104) Bq/kg and (1694±220) Bq/kg, respectively. Further analysis showed increased gross activities of radionuclides of the 238U chain, while the radionuclides of the 232Th chain and 40K were below the detection limit. The mean values of the specific activities of 238U and 226Ra were (360±55) Bq/kg and (280±84) Bq/kg, respectively. The estimated annual outdoor effective dose, at 1m received by adults was 0.25 mSv/y, which is below a dose limit of 1 mSv/y for members of general public. The results obtained in this study show that radionuclides, although present in relatively high concentrations in the phosphogypsum pile, do not imply an increased external radiation risk for members of the population. The possible use of phosphogypsum in civil construction and agriculture may not be excluded if conditions of prior good planning taking into account the radionuclides activities exist.

Sign in / Sign up

Export Citation Format

Share Document