Intestinal Stromal Tumors in a Simian Immunodeficiency Virus-infected, Simian Retrovirus-2 Negative Rhesus Macaque (Macaca mulatta)

2005 ◽  
Vol 42 (3) ◽  
pp. 391-396 ◽  
Author(s):  
H. Bielefeldt-Ohmann ◽  
D. H. Barouch ◽  
A. M. Bakke ◽  
A. G. Bruce ◽  
M. Durning ◽  
...  
Keyword(s):  
Rhesus Macaque ◽  
Simian Immunodeficiency Virus ◽  
Macaca Mulatta ◽  
Human Herpesvirus ◽  
Kaposi Sarcoma ◽  
Polymerase Chain Reaction Analysis ◽  
Nuclear Antigen ◽  
Stromal Tumors ◽  
Immunodeficiency Virus ◽  
Absolute Identity

Multifocal submucosal stromal tumors were diagnosed in a 5.5-year-old rhesus macaque ( Macaca mulatta) experimentally infected with simian immunodeficiency virus, strain SIVsmE660, and CD4+ T cell depleted. The animal was negative for simian retroviruses, SRV-1, -2, and -5. Polymerase chain reaction analysis of DNA from tumor and spleen tissue revealed abundant, preferential presence of retroperitoneal fibromatosis herpesvirus, the macaque homologue of the Kaposi sarcoma-associated herpesvirus (human herpesvirus-8), in the tumors. This was corroborated by demonstration of viral latent nuclear antigen-1 in the nuclei of a majority of the spindeloid tumor cells. Low levels of an additional macaque herpesvirus, rhesus rhadinovirus, were also detected in the spleen and tumor tissues. The spindeloid cells labeled positively for vimentin and CD117 but were negative for CD31, CD68, desmin, and smooth muscle cell actin. Collectively, these findings suggest a relation to but not absolute identity with simian mesenchymoproliferative disorders (MPD) or typical gastrointestinal stromal tumors (GISTs).

scholarly journals Efficacy of 6-chloro-2',3'-dideoxyguanosine(6-Cl-ddG) on an ARC/AIDS Rhesus macaque (Macaca mulatta) Infected with Simian Immunodeficiency Virus.

1997 ◽  
Vol 46 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Yuji FUJII ◽  
Ryouzaburou MUKAI ◽  
Yuichi MURAYAMA ◽  
Hirofumi AKARI ◽  
Makoto MACHIDA ◽  
...  
Keyword(s):  
Rhesus Macaque ◽  
Simian Immunodeficiency Virus ◽  
Macaca Mulatta ◽  
Immunodeficiency Virus

scholarly journals Ulcerative Cheilitis in a Rhesus Macaque

2011 ◽  
Vol 49 (2) ◽  
pp. 412-415 ◽  
Author(s):  
C. C. Bailey ◽  
A. D. Miller
Keyword(s):  
Epithelial Cells ◽  
Rhesus Macaque ◽  
Macaca Mulatta ◽  
Inclusion Bodies ◽  
Human Herpesvirus ◽  
Large Numbers ◽  
B Virus ◽  
Immunodeficiency Virus ◽  
History Of ◽  
Herpesvirus 1

A 2-year-old, female, simian immunodeficiency virus E543–infected rhesus macaque ( Macaca mulatta) was presented for necropsy following euthanasia due to a history of diarrhea, weight loss, and a small, round ulcer along the left labial commissure. Histopathologic examination of the ulcer revealed infiltration by large numbers of degenerate and nondegenerate neutrophils and macrophages admixed with syncytial epithelial cells. Rare epithelial cells contained herpetic inclusion bodies. These cells stained positive for Human herpesvirus 1 via immunohistochemistry, and DNA sequencing confirmed the presence of closely related Macacine herpesvirus 1 (B virus).

scholarly journals Immunohistochemical detection of the latent nuclear antigen-1 of the human herpesvirus type 8 to differentiate cutaneous epidemic Kaposi sarcoma and its histological simulators

2013 ◽  
Vol 88 (2) ◽  
pp. 243-246 ◽  
Author(s):  
Patricia Fonseca Pereira ◽  
Tullia Cuzzi ◽  
Maria Clara Gutierrez Galhardo
Keyword(s):  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Nuclear Antigen ◽  
Immunohistochemical Detection ◽  
Herpesvirus Type ◽  
Human Herpesvirus Type ◽  
Skin Biopsies ◽  
Latent Nuclear Antigen

Kaposi's sarcoma is the most common neoplasia diagnosed in AIDS patients and the expression of the human herpesvirus-8 (HHV-8) latent nuclear antigen-1 has been useful for its histological diagnosis. The aim of this study is to confirm that immunohistochemistry is a valuable tool for differentiating KS from its simulators in skin biopsies of HIV patients. Immunohistochemical and histological analyses were performed in 49 Kaposi's sarcoma skin biopsies and 60 of its histological simulators. Positivity was present in the 49 Kaposi's sarcoma skin biopsies and no staining was observed in the 60 simulators analyzed, resulting in sensibility and specificity of 100%. HHV-8 immunohistochemical detection is an effective tool for diagnosing Kaposi's sarcoma, especially in early lesions in which neoplastic features are not evident. It also contributes to its histological differential diagnosis.

Expression of the Interleukin-18 Gene from Rhesus Macaque by the Simian Immunodeficiency Virus Does Not Result in Increased Viral Replication

2001 ◽  
Vol 21 (3) ◽  
pp. 173-180 ◽  
Author(s):  
Luis D. Giavedoni ◽  
Jennifer D. Imhoof ◽  
M. Cristina Velasquillo ◽  
Laura M. Parodi ◽  
Vida L. Hodara
Keyword(s):  
Viral Replication ◽  
Rhesus Macaque ◽  
Simian Immunodeficiency Virus ◽  
Interleukin 18 ◽  
Immunodeficiency Virus

scholarly journals Role of rhesus macaque IFITM3(2) in simian immunodeficiency virus infection of macaques

PLoS ONE ◽  
2019 ◽  
Vol 14 (11) ◽  
pp. e0224082
Author(s):  
Michael Winkler ◽  
Sabine Gärtner ◽  
Lara Markus ◽  
Markus Hoffmann ◽  
Inga Nehlmeier ◽  
...  
Keyword(s):  
Virus Infection ◽  
Rhesus Macaque ◽  
Simian Immunodeficiency Virus ◽  
Simian Immunodeficiency Virus Infection ◽  
Immunodeficiency Virus

scholarly journals Route of Simian Immunodeficiency Virus Inoculation Determines the Complexity but Not the Identity of Viral Variant Populations That Infect Rhesus Macaques

2001 ◽  
Vol 75 (8) ◽  
pp. 3753-3765 ◽  
Author(s):  
Jennifer L. Greenier ◽  
Christopher J. Miller ◽  
Ding Lu ◽  
Peter J. Dailey ◽  
Fabien X. Lü ◽  
...  
Keyword(s):  
Rhesus Macaque ◽  
Simian Immunodeficiency Virus ◽  
Hiv Transmission ◽  
Rhesus Macaques ◽  
Systemic Infection ◽  
Envelope Gene ◽  
Homogeneous Population ◽  
Viral Variant ◽  
Effective Strategies ◽  
Immunodeficiency Virus

ABSTRACT A better understanding of the host and viral factors associated with human immunodeficiency virus (HIV) transmission is essential to developing effective strategies to curb the global HIV epidemic. Here we used the rhesus macaque-simian immunodeficiency virus (SIV) animal model of HIV infection to study the range of viral genotypes that are transmitted by different routes of inoculation and by different types of viral inocula. Analysis of transmitted variants was undertaken in outbred rhesus macaques inoculated intravenously (IV) or intravaginally (IVAG) with a genetically heterogeneous SIVmac251 stock derived from a well-characterized rhesus macaque viral isolate. In addition, we performed serial IV and IVAG passage experiments using plasma from SIV-infected macaques as the inoculum. We analyzed the V1-V2 region of the SIV envelope gene from virion-associated RNA in plasma from infected animals by the heteroduplex mobility assay (HMA) and by DNA sequence analysis. We found that a more diverse population of SIV genetic variants was present in the earliest virus-positive plasma samples from all five IV SIVmac251-inoculated monkeys and from two of five IVAG SIVmac251-inoculated monkeys. In contrast, we found a relatively homogeneous population of SIV envelope variants in three of five monkeys inoculated IVAG with SIVmac251 stock and in two monkeys infected after IVAG inoculation with plasma from an SIV-infected animal. In some IVAG-inoculated animals, the transmitted SIV variant was the most common variant in the inoculum. However, a specific viral variant in the SIVmac251 stock was not consistently transmitted by IVAG inoculation. Thus, it is likely that host factors or stochastic processes determine the specific viral variants that infect an animal after IVAG SIV exposure. In addition, our results clearly demonstrate that the route of inoculation is associated with the extent and breadth of the genetic complexity of the viral variant population in the earliest stages of systemic infection.

Sign in / Sign up

Export Citation Format

Share Document