scholarly journals Mechanism of Mechanically Induced Intercellular Calcium Waves in Rabbit Articular Chondrocytes and in HIG-82 Synovial Cells

1998 ◽  
Vol 13 (3) ◽  
pp. 443-453 ◽  
Author(s):  
Micaela Grandolfo ◽  
Alessandra Calabrese ◽  
Paola D'andrea
Keyword(s):  
Articular Chondrocytes ◽  
Calcium Waves ◽  
Synovial Cells ◽  
Rabbit Articular Chondrocytes

scholarly journals Intercellular calcium signalling between chondrocytes and synovial cells in co-culture

1998 ◽  
Vol 329 (3) ◽  
pp. 681-687 ◽  
Author(s):  
Paola D'ANDREA ◽  
Alessandra CALABRESE ◽  
Micaela GRANDOLFO
Keyword(s):  
Gap Junctions ◽  
Intercellular Communication ◽  
Structural Changes ◽  
Articular Chondrocytes ◽  
Cell Metabolism ◽  
Second Messengers ◽  
Calcium Signalling ◽  
Cell Types ◽  
Synovial Cells ◽  
Cell Coupling

Intercellular communication allows the co-ordination of cell metabolism between tissues as well as sensitivity to extracellular stimuli. Paracrine stimulation and cell-to-cell coupling through gap junctions induce the formation of complex cellular networks that favour the intercellular exchange of nutrients and second messengers. Heterologous intercellular communication was studied in co-cultures of articular chondrocytes and HIG-82 synovial cells by measuring mechanically induced cytosolic changes in Ca2+ ion levels by digital fluorescence video imaging. In confluent co-cultures, mechanical stimulation induced intercellular Ca2+ waves that propagated to both cell types with similar kinetics. Intercellular wave spreading was inhibited by 18α-glycyrrhetinic acid and by treatments inhibiting the activation of purinoreceptors, suggesting that intercellular signalling between these two cell types occurs both through gap junctions and ATP-mediated paracrine stimulation. In rheumatoid arthritis the formation of the synovial pannus induces structural changes at the chondrosynovial junction, where chondrocyte and synovial cells come into close apposition: these results provide the first evidence for direct intercellular communication between these two cell types.

SV40-immortalization of rabbit articular chondrocytes: Alteration of differentiated functions

1992 ◽  
Vol 150 (1) ◽  
pp. 158-167 ◽  
Author(s):  
S. Thenet ◽  
P. D. Benya ◽  
S. Demignot ◽  
J. Feunteun ◽  
M. Adolphe
Keyword(s):  
Articular Chondrocytes ◽  
Rabbit Articular Chondrocytes

Subculture of rabbit articular chondrocytes within a collagen gel: growth and analysis of differentiation

1988 ◽  
Vol 1 (2) ◽  
pp. 123-132 ◽  
Author(s):  
Benoit Dewilde ◽  
Laurent Benel ◽  
Daniel-Jean Hartmann ◽  
Monique Adolphe
Keyword(s):  
Articular Chondrocytes ◽  
Collagen Gel ◽  
Gel Growth ◽  
Rabbit Articular Chondrocytes

Effects of antiinflammatory drugs on soft agar growth of rabbit articular chondrocytes

1989 ◽  
Vol 18 (3) ◽  
pp. 7-10 ◽  
Author(s):  
Yukio Kato ◽  
Katsuhiko Sato ◽  
Tatsuya Koike ◽  
Kazuhisha Nakashima ◽  
Wei Qun Yen ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Soft Agar ◽  
Antiinflammatory Drugs ◽  
Soft Agar Growth ◽  
Rabbit Articular Chondrocytes
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