Modulation of extracellular matrix metabolism in rabbit articular chondrocytes and human rheumatoid synovial cells by the non-steroidal anti-inflammatory drug etodolac. I: Collagen synthesis

1990 ◽  
Vol 31 (3-4) ◽  
pp. 345-352 ◽  
Author(s):  
A. Mauviel ◽  
F. Redini ◽  
G. Loyau ◽  
J. -P. Pujol
Keyword(s):  
Extracellular Matrix ◽  
Collagen Synthesis ◽  
Articular Chondrocytes ◽  
Synovial Cells ◽  
Inflammatory Drug ◽  
Anti Inflammatory ◽  
Matrix Metabolism ◽  
Rabbit Articular Chondrocytes

scholarly journals Effects of anti-inflammatory drugs on matrix degradation of rabbit articular chondrocytes in vitro.

Ensho ◽  
1986 ◽  
Vol 6 (1) ◽  
pp. 71-73
Author(s):  
Katsuhiro Shimada ◽  
Masayuki Shinmei ◽  
Toshiyuki Kikuchi ◽  
Koichi Masuda ◽  
Yutaka Shimomura ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Matrix Degradation ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Rabbit Articular Chondrocytes

scholarly journals Rapid and inexpensive method of studying dedifferentiation in rabbit articular chondrocytes

2021 ◽  
Author(s):  
Fahad Hassan Shah ◽  
Young Seok Eom ◽  
Byung Su Ko ◽  
Yohan Han ◽  
Song Ja Kim
Keyword(s):  
Extracellular Matrix ◽  
Articular Chondrocytes ◽  
Degenerative Diseases ◽  
Cell Models ◽  
Inexpensive Method ◽  
Rabbit Articular Chondrocytes ◽  
Dedifferentiation Process

Abstract Chondrocyte dedifferentiation is a cellular phenomenon in which chondrocyte loses its chondrogenic characteristics and ability to synthesize extracellular matrix. These conditions commonly reported in different cartilage degenerative diseases. The methods used to induce dedifferentiation in cell models of chondrocytes are often time consuming or require expensive reagents. Here in our protocol, we describe the utility of using 2-deoxy-D-glucose as a dedifferentiating agent for rabbit chondrocytes for studying dedifferentiation process.

scholarly journals The effects of 1-hydroxyethane-1,1-diphosphonate and dichloromethanediphosphonate on collagen synthesis by rabbit articular chondrocytes and rat bone cells

1981 ◽  
Vol 196 (1) ◽  
pp. 293-301 ◽  
Author(s):  
H L Guenther ◽  
H E Guenther ◽  
H Fleisch
Keyword(s):  
Collagen Synthesis ◽  
Articular Chondrocytes ◽  
Bone Cells ◽  
Radioactive Material ◽  
Secondary Culture ◽  
Positive Effect ◽  
Rabbit Articular Chondrocytes ◽  
Isolated Rat ◽  
Rat Bone

Investigations were performed to assess the effects of dichloromethanediphosphonate on the synthesis of collagen by (1) isolated rabbit articular chondrocytes, (2) isolated rat calvaria bone cells and (3) bone explants from rats treated with the diphosphonates. The studies showed that dichloromethanediphosphonate, but not 1-hydroxyethane-1,1-diphosphonate, causes articular chondrocytes to increase net collagen biosynthesis, both when measured as 3H-labelled or as non-radioactive material, in a dose-related fashion. The increment in collagen synthesis was still evident with cells that were exposed continuously to the diphosphonate in primary as well as secondary culture; however, it declined with cells in tertiary culture and was absent after the fourth subculture. The type of collagen was not affected by the diphosphonate. The synthesis of collagen by bone cells was likewise increased with dichloromethanediphosphonate. No effects were detected with 1-hydroxyethane-1,1-diphosphonate was tested. Finally, when calvaria and tibiae from diphosphonate-treated rats were cultured in vitro, the positive effect of dichloromethanediphosphonate on collagen synthesis was also evident. 1-Hydroxyethane-1,1-diphosphonate, on the other hand, decreased the incorporation of [3H]proline into the collagen of calvaria and osseous tibial shafts and showed no effect on the collagen synthesis of the cartilaginous tibial heads.

scholarly journals Metabolic Effects of Avocado/Soy Unsaponifiables on Articular Chondrocytes

2008 ◽  
Vol 5 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Louis Lippiello ◽  
Joseph V. Nardo ◽  
Robert Harlan ◽  
Tiffany Chiou
Keyword(s):  
Collagen Synthesis ◽  
Articular Chondrocytes ◽  
Interleukin 1 ◽  
Metabolic Effects ◽  
Sterol Content ◽  
Similar Time ◽  
Anabolic Activity ◽  
Biologic Effects ◽  
Anti Inflammatory

Avocado/soy unsaponifiable (ASU) components are reported to have a chondroprotective effect by virtue of anti-inflammatory and proanabolic effects on articular chondrocytes. The identity of the active component(s) remains unknown. In general, sterols, the major component of unsaponifiable plant material have been demonstrated to be anti-inflammatoryin vitroand in animal models. These studies were designed to clarify whether the sterol content of ASU preparations were the primary contributors to biological activity in articular chondrocytes. ASU samples were analyzed by high pressure liquid chromatography (HPLC) and GC mass spectrometry. The sterol content was normalized between diverse samples prior toin vitrotesting on bovine chondrocytes. Anabolic activity was monitored by uptake of 35-sulfate into proteoglycans and quantitation of labeled hydroxyproline and proline content after incubation with labeled proline. Anti-inflammatory activity was assayed by measuring reduction of interleukin-1 (IL-1)-induced synthesis of PGE2 and metalloproteases and release of label from tissue prelabeled with S-35.All ASU samples exerted a similar time-dependent up-regulation of 35-sulfate uptake in bovine cells reaching a maximum of greater than 100% after 72 h at sterol doses of 1–10 μg/ml. Non-collagenous protein (NCP) and collagen synthesis were similarly up-regulated. All ASU were equally effective in dose dependently inhibiting IL-1-induced MMP-3 activity (23–37%), labeled sulfate release (15–23%) and PGE2 synthesis (45–58%). Up-regulation of glycosaminoglycan and collagen synthesis and reduction of IL-1 effects in cartilage are consistent with chondroprotective activity. The similarity of activity of ASU from diverse sources when tested at equal sterol levels suggests sterols are important for biologic effects in articular chondrocytes.

Synovium stem cell-derived matrix enhances anti-inflammatory properties of rabbit articular chondrocytes via the SIRT1 pathway

2020 ◽  
Vol 106 ◽  
pp. 110286 ◽  
Author(s):  
Jinku Yan ◽  
Xi Chen ◽  
Chengbo Pu ◽  
Yilang Zhao ◽  
Xiaozhen Liu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Articular Chondrocytes ◽  
Anti Inflammatory ◽  
Rabbit Articular Chondrocytes
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