scholarly journals Involvement of Cyclic Adenosine Monophosphate-Dependent Protein Kinase A and Pertussis Toxin-Sensitive G Proteins in the Migratory Response of Human CD14+ Mononuclear Cells to Katacalcin

2002 ◽  
Vol 17 (10) ◽  
pp. 1872-1882 ◽  
Author(s):  
Nicole C. Kaneider ◽  
Petra Egger ◽  
Franz J. Wiedermann ◽  
Markus Ritter ◽  
Ewald Wöll ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
G Proteins ◽  
Mononuclear Cells ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Dependent Protein Kinase ◽  
Migratory Response ◽  
Dependent Protein ◽  
Kinase A

scholarly journals The 3-phosphoinositide–dependent protein kinase 1 is an essential upstream activator of protein kinase A in malaria parasites

PLoS Biology ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. e3001483
Author(s):  
Eva Hitz ◽  
Natalie Wiedemar ◽  
Armin Passecker ◽  
Beatriz A. S. Graça ◽  
Christian Scheurer ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Blood Stage ◽  
Targeted Mutagenesis ◽  
Missense Mutations ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Kinase A

Cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) signalling is essential for the proliferation of Plasmodium falciparum malaria blood stage parasites. The mechanisms regulating the activity of the catalytic subunit PfPKAc, however, are only partially understood, and PfPKAc function has not been investigated in gametocytes, the sexual blood stage forms that are essential for malaria transmission. By studying a conditional PfPKAc knockdown (cKD) mutant, we confirm the essential role for PfPKAc in erythrocyte invasion by merozoites and show that PfPKAc is involved in regulating gametocyte deformability. We furthermore demonstrate that overexpression of PfPKAc is lethal and kills parasites at the early phase of schizogony. Strikingly, whole genome sequencing (WGS) of parasite mutants selected to tolerate increased PfPKAc expression levels identified missense mutations exclusively in the gene encoding the parasite orthologue of 3-phosphoinositide–dependent protein kinase-1 (PfPDK1). Using targeted mutagenesis, we demonstrate that PfPDK1 is required to activate PfPKAc and that T189 in the PfPKAc activation loop is the crucial target residue in this process. In summary, our results corroborate the importance of tight regulation of PfPKA signalling for parasite survival and imply that PfPDK1 acts as a crucial upstream regulator in this pathway and potential new drug target.

scholarly journals Increased expression of the RIα subunit of the cAMP-dependent protein kinase A is associated with advanced stage ovarian cancer

1999 ◽  
Vol 79 (5-6) ◽  
pp. 933-939 ◽  
Author(s):  
H M McDaid ◽  
M T Cairns ◽  
R J Atkinson ◽  
S McAleer ◽  
D P Harkin ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Advanced Stage ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein ◽  
Kinase A

Impaired G-protein-stimulated adenylyl cyclase activity in Alzheimer's disease brain is not accompanied by reduced cyclic-AMP-dependent protein kinase A activity

1996 ◽  
Vol 737 (1-2) ◽  
pp. 155-161 ◽  
Author(s):  
Willy L Bonkale ◽  
Johan Fastbom ◽  
Birgitta Wiehager ◽  
Rivka Ravid ◽  
Bengt Winblad ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Protein Kinase A ◽  
Adenylyl Cyclase ◽  
G Protein ◽  
Cyclase Activity ◽  
Adenylyl Cyclase Activity ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Kinase A
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