Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers

A total of 266 Martonvásár (Mv) wheat (Triticum aestivum L.) accessions, including varieties and advanced lines, were examined using the “Perfect” molecular markers to detect the Rht-B1b (formerly Rht1) and Rht-D1b (formerly Rht2) semi-dwarfing genes. The gene Rht-B1b was detected in a total of 221 (83.5%) accessions. The Rht-D1b allele was found in fewer accessions. Overall 24 genotypes (9%) contained this allele. The analysis of the development date of the genotypes revealed that the introduction of the dwarfing genes into Martonvásár breeding programmes started in the early 1970s, and they were widely utilized from the 1980s. The Rht-B1b allele was the main source for reducing plant height, while the Rht-D1b allele played only a minor role in the Martonvásár breeding programme.Characterizing accessions using various molecular markers allows us to create a database offering relevant marker information about genotypes. Such a database could be very helpful for selection, allowing breeders to include varieties giving positive results in specific breeding programmes.

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Characterization of wheat (Triticum aestivum L.) genotypes unraveled by molecular markers considering heat stress

Open Agriculture ◽

10.1515/opag-2019-0037 ◽

2019 ◽

Vol 4 (1) ◽

pp. 374

Author(s):

Yaswant Kumar Pankaj ◽

Mahesh Jagadale Vasantrao ◽

Nilmani Prakash ◽

Raj Kumar Jat ◽

Rajesh Kumar ◽

...

Keyword(s):

Triticum Aestivum ◽

Heat Stress ◽

Molecular Markers ◽

Triticum Aestivum L

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Genetic analysis and gene detection of fructan content using DArT molecular markers in spring bread wheat (Triticum aestivum L.) grain

Molecular Breeding ◽

10.1007/s11032-020-1102-4 ◽

2020 ◽

Vol 40 (2) ◽

Author(s):

Zhankui Zeng ◽

Chunping Wang ◽

Zhenghong Wang ◽

Zhipeng Han ◽

Liming Wang ◽

...

Keyword(s):

Triticum Aestivum ◽

Molecular Markers ◽

Genetic Analysis ◽

Bread Wheat ◽

Triticum Aestivum L ◽

Gene Detection ◽

Spring Bread Wheat

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Triticum aestivum × Triticum timopheevii introgression lines as a source of pathogen resistance genes

Czech Journal of Genetics and Plant Breeding ◽

10.17221/3254-cjgpb ◽

2011 ◽

Vol 47 (Special Issue) ◽

pp. S49-S55 ◽

Cited By ~ 9

Author(s):

I.N. Leonova ◽

E.B. Budashkina ◽

N.P. Kalinina ◽

M.S. Röder ◽

A. Börner ◽

...

Keyword(s):

Triticum Aestivum ◽

Microsatellite Markers ◽

Molecular Mapping ◽

Tetraploid Wheat ◽

Leaf Rust Resistance ◽

Pathogen Resistance ◽

Introgression Lines ◽

Phenotypic Variance ◽

Triticum Timopheevii ◽

Loose Smut

A collection of introgression lines was obtained from crosses of common wheat (Triticum aestivum L.) cultivars with tetraploid wheat Triticum timopheevii (Zhuk.). Evaluation of resistance to fungal diseases revealed the lines with resistance to leaf and stem rusts, powdery mildew, spot blotch, and loose smut, the most widespread in Siberian region of Russia. Localization of the T. timopheevii genome fragments by means of microsatellite markers determined higher frequency of substitutions and translocations on chromosomes 1А, 2A, 2B, 5A, 5B and 6B. Molecular mapping of the loci determining leaf rust resistance revealed two independent loci on chromosomes 5B and 2A. The major locus on 5BS.5BL-5GL translocated chromosome accounting 64% of the phenotypic variance of the trait was found to be closely linked to microsatellite markers Xgwm814 and Xgwm1257. The other, minor locus, controlling 11% of the trait was mapped next to Xgwm312 on chromosome 2A. Microsatellite markers located near these genes may be used for controlling the transfer of valuable traits in new wheat cultivars.

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