Computer-generated Fresnel Hologram Using Multiple Angular Orthogonal Projection Images

Author(s):  
Xuemei Cao ◽  
Xinzhu Sang ◽  
Zhidong Chen ◽  
Junmin Leng ◽  
Ming Zhang



2014 ◽  
Vol 324 ◽  
pp. 47-52 ◽  
Author(s):  
Xuemei Cao ◽  
Xinzhu Sang ◽  
Zhidong Chen ◽  
Junmin Leng ◽  
Binbin Yan ◽  
...  


2014 ◽  
Vol 50 ◽  
pp. 72-81 ◽  
Author(s):  
M. Pamplona Segundo ◽  
L. Silva ◽  
O.R.P. Bellon ◽  
S. Sarkar


Author(s):  
D.W. Andrews ◽  
F.P. Ottensmeyer

Shadowing with heavy metals has been used for many years to enhance the topological features of biological macromolecular complexes. The three dimensional features present in directionaly shadowed specimens often simplifies interpretation of projection images provided by other techniques. One difficulty with the method is the relatively large amount of metal used to achieve sufficient contrast in bright field images. Thick shadow films are undesirable because they decrease resolution due to an increased tendency for microcrystalline aggregates to form, because decoration artefacts become more severe and increased cap thickness makes estimation of dimensions more uncertain.The large increase in contrast provided by the dark field mode of imaging allows the use of shadow replicas with a much lower average mass thickness. To form the images in Fig. 1, latex spheres of 0.087 μ average diameter were unidirectionally shadowed with platinum carbon (Pt-C) and a thin film of carbon was indirectly evaporated on the specimen as a support.



Author(s):  
B. Carragher ◽  
M. Whittaker

Techniques for three-dimensional reconstruction of macromolecular complexes from electron micrographs have been successfully used for many years. These include methods which take advantage of the natural symmetry properties of the structure (for example helical or icosahedral) as well as those that use single axis or other tilting geometries to reconstruct from a set of projection images. These techniques have traditionally relied on a very experienced operator to manually perform the often numerous and time consuming steps required to obtain the final reconstruction. While the guidance and oversight of an experienced and critical operator will always be an essential component of these techniques, recent advances in computer technology, microprocessor controlled microscopes and the availability of high quality CCD cameras have provided the means to automate many of the individual steps.During the acquisition of data automation provides benefits not only in terms of convenience and time saving but also in circumstances where manual procedures limit the quality of the final reconstruction.



2013 ◽  
Vol E96.B (5) ◽  
pp. 1215-1217 ◽  
Author(s):  
Ann-Chen CHANG ◽  
Chih-Chang SHEN


2014 ◽  
Vol 35 (1) ◽  
pp. 24-28
Author(s):  
Yong Yang ◽  
Shun-ping Xiao ◽  
De-jun Feng ◽  
Wen-ming Zhang


2020 ◽  
Vol 17 (1) ◽  
pp. 87-94
Author(s):  
Ibrahim A. Naguib ◽  
Fatma F. Abdallah ◽  
Aml A. Emam ◽  
Eglal A. Abdelaleem

: Quantitative determination of pyridostigmine bromide in the presence of its two related substances; impurity A and impurity B was considered as a case study to construct the comparison. Introduction: Novel manipulations of the well-known classical least squares multivariate calibration model were explained in detail as a comparative analytical study in this research work. In addition to the application of plain classical least squares model, two preprocessing steps were tried, where prior to modeling with classical least squares, first derivatization and orthogonal projection to latent structures were applied to produce two novel manipulations of the classical least square-based model. Moreover, spectral residual augmented classical least squares model is included in the present comparative study. Methods: 3 factor 4 level design was implemented constructing a training set of 16 mixtures with different concentrations of the studied components. To investigate the predictive ability of the studied models; a test set consisting of 9 mixtures was constructed. Results: The key performance indicator of this comparative study was the root mean square error of prediction for the independent test set mixtures, where it was found 1.367 when classical least squares applied with no preprocessing method, 1.352 when first derivative data was implemented, 0.2100 when orthogonal projection to latent structures preprocessing method was applied and 0.2747 when spectral residual augmented classical least squares was performed. Conclusion: Coupling of classical least squares model with orthogonal projection to latent structures preprocessing method produced significant improvement of the predictive ability of it.



Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3652
Author(s):  
Cory Juntunen ◽  
Isabel M. Woller ◽  
Yongjin Sung

Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.



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