scholarly journals Video-rate Volumetric Neuronal Imaging Using 3D Targeted Illumination

Author(s):  
Sheng Xiao ◽  
Hua-an Tseng ◽  
Howard Gritton ◽  
Xue Han ◽  
Jerome Mertz
Keyword(s):  
2010 ◽  
Vol 55 (10) ◽  
pp. A88.E832 ◽  
Author(s):  
Ming Yu ◽  
Mary Guaraldi ◽  
Jody Bozek ◽  
Melanie Lamoy ◽  
Paula Silva ◽  
...  
Keyword(s):  

2013 ◽  
Vol 40 (5) ◽  
pp. 682-688 ◽  
Author(s):  
Ming Yu ◽  
Jody Bozek ◽  
Mikhail Kagan ◽  
Mary Guaraldi ◽  
Paula Silva ◽  
...  

Author(s):  
Erich E. Hoover ◽  
Michael D. Young ◽  
Susy M. Kim ◽  
Eric V. Chandler ◽  
Jeffrey J. Field ◽  
...  

2014 ◽  
Vol 136 (13) ◽  
pp. 4877-4880 ◽  
Author(s):  
Kenneth S. Hettie ◽  
Jessica L. Klockow ◽  
Timothy E. Glass

2008 ◽  
Vol 99 (6) ◽  
pp. 3136-3143 ◽  
Author(s):  
S. R. Lockery ◽  
K. J. Lawton ◽  
J. C. Doll ◽  
S. Faumont ◽  
S. M. Coulthard ◽  
...  

With a nervous system of only 302 neurons, the free-living nematode Caenorhabditis elegans is a powerful experimental organism for neurobiology. However, the laboratory substrate commonly used in C. elegans research, a planar agarose surface, fails to reflect the complexity of this organism's natural environment, complicates stimulus delivery, and is incompatible with high-resolution optophysiology experiments. Here we present a new class of microfluidic devices for C. elegans neurobiology and behavior: agarose-free, micron-scale chambers and channels that allow the animals to crawl as they would on agarose. One such device mimics a moist soil matrix and facilitates rapid delivery of fluid-borne stimuli. A second device consists of sinusoidal channels that can be used to regulate the waveform and trajectory of crawling worms. Both devices are thin and transparent, rendering them compatible with high-resolution microscope objectives for neuronal imaging and optical recording. Together, the new devices are likely to accelerate studies of the neuronal basis of behavior in C. elegans.


2015 ◽  
Vol 2 (3) ◽  
pp. 031203 ◽  
Author(s):  
Yi Shen ◽  
Tiffany Lai ◽  
Robert E. Campbell

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