scholarly journals Visualizing Mammalian Brain Area Interactions by Dual-axis Two-photon Calcium Imaging

CLEO: 2015 ◽  
2015 ◽  
Author(s):  
Mark Schnitzer
Keyword(s):  
Calcium Imaging ◽  
Brain Area ◽  
Mammalian Brain ◽  
Two Photon

scholarly journals Visualizing mammalian brain area interactions by dual-axis two-photon calcium imaging

2014 ◽  
Vol 17 (12) ◽  
pp. 1825-1829 ◽  
Author(s):  
Jérôme Lecoq ◽  
Joan Savall ◽  
Dejan Vučinić ◽  
Benjamin F Grewe ◽  
Hyun Kim ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Brain Area ◽  
Mammalian Brain ◽  
Two Photon

scholarly journals Quantitative evaluation of two-photon calcium imaging modalities for high-speed volumetric calcium imaging in scattering brain tissue

2017 ◽  
Author(s):  
Siegfried Weisenburger ◽  
Robert Prevedel ◽  
Alipasha Vaziri
Keyword(s):  
Brain Tissue ◽  
Quantitative Evaluation ◽  
Calcium Imaging ◽  
Model Systems ◽  
Network Activity ◽  
Mammalian Brain ◽  
Optimization Approach ◽  
Wide Field ◽  
Two Photon ◽  
Neuronal Network Activity

AbstractConsiderable efforts are currently being devoted to enhance the speed, spatial resolution and the size of the 3D sample volumes in which calcium imaging methods can capture neuronal network activity in different model systems. In the mammalian brain, tissue scattering severely limits the use of parallel acquisition techniques such as wide-field imaging and, as a consequence, methods based on two-photon point-scanning (2PM) have become the method of choice. However, 2PM faces severe restrictions due to technical limitations such as scan speed, laser power, and those related to the fluorescent probes, calling for conceptually new approaches to enhance the performance of two-photon calcium imaging schemes. Here we provide a detailed quantitative evaluation and comparison of different excitation/detection modalities from the perspective of detecting neuronal activity that are based on different point-spread functions (PSF), laser repetition rates and sampling strategies. We demonstrate the conditions for which imaging speed and signal-to-noise ratio are optimized for a given average power. Our results are based on numerical simulations which are informed by experimentally measured parameters and show that volumetric field of view and acquisition speed can be considerably improved compared to traditional 2PM schemes by a holistic optimization approach.

scholarly journals Long-range population dynamics of anatomically defined neocortical networks

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Jerry L Chen ◽  
Fabian F Voigt ◽  
Mitra Javadzadeh ◽  
Roland Krueppel ◽  
Fritjof Helmchen
Keyword(s):  
Population Dynamics ◽  
Long Range ◽  
Calcium Imaging ◽  
Brain Function ◽  
Motor Behavior ◽  
Mammalian Brain ◽  
Projection Neurons ◽  
Motor Behaviors ◽  
Two Photon ◽  
Sensory Cortices

The coordination of activity across neocortical areas is essential for mammalian brain function. Understanding this process requires simultaneous functional measurements across the cortex. In order to dissociate direct cortico-cortical interactions from other sources of neuronal correlations, it is furthermore desirable to target cross-areal recordings to neuronal subpopulations that anatomically project between areas. Here, we combined anatomical tracers with a novel multi-area two-photon microscope to perform simultaneous calcium imaging across mouse primary (S1) and secondary (S2) somatosensory whisker cortex during texture discrimination behavior, specifically identifying feedforward and feedback neurons. We find that coordination of S1-S2 activity increases during motor behaviors such as goal-directed whisking and licking. This effect was not specific to identified feedforward and feedback neurons. However, these mutually projecting neurons especially participated in inter-areal coordination when motor behavior was paired with whisker-texture touches, suggesting that direct S1-S2 interactions are sensory-dependent. Our results demonstrate specific functional coordination of anatomically-identified projection neurons across sensory cortices.

scholarly journals High resolution ultrasonic neural modulation observed via in vivo two-photon calcium imaging

2021 ◽  
Author(s):  
Zongyue Cheng ◽  
Chenmao Wang ◽  
Bowen Wei ◽  
Wenbiao Gan ◽  
Qifa Zhou ◽  
...  
Keyword(s):  
High Resolution ◽  
Calcium Imaging ◽  
Focused Ultrasound ◽  
Neuronal Response ◽  
Mammalian Brain ◽  
Label Free ◽  
High Frequency Ultrasound ◽  
Two Photon ◽  
Neural Modulation

Neural modulation plays a major role in delineating the circuit mechanisms and serves as the cornerstone of neural interface technologies. Among the various modulation mechanisms, ultrasound enables noninvasive label-free deep access to mammalian brain tissue. To date, most if not all ultrasonic neural modulation implementations are based on ~1 MHz carrier frequency. The long acoustic wavelength results in a spatially coarse modulation zone, often spanning over multiple function regions. The modulation of one brain region is inevitably linked with the modulation of its neighboring regions. To significantly increase the spatial resolution, we explored the application of high-frequency ultrasound. To investigate the neuronal response at cellular resolutions, we developed a dual-modality system combining in vivo two-photon calcium imaging and focused ultrasound modulation. The studies show that the ~30 MHz ultrasound can suppress the neuronal activity in awake mice at 100-micron scale spatial resolutions, paving the way for high-resolution ultrasonic neural modulation.

scholarly journals Two-photon calcium imaging during fictive navigation in virtual environments

2013 ◽  
Vol 7 ◽  
Author(s):  
Misha B. Ahrens ◽  
Kuo Hua Huang ◽  
Sujatha Narayan ◽  
Brett D. Mensh ◽  
Florian Engert
Keyword(s):  
Virtual Environments ◽  
Calcium Imaging ◽  
Two Photon

scholarly journals Functional Microarchitecture of the Mouse Dorsal Inferior Colliculus Revealed through In Vivo Two-Photon Calcium Imaging

2015 ◽  
Vol 35 (31) ◽  
pp. 10927-10939 ◽  
Author(s):  
O. Barnstedt ◽  
P. Keating ◽  
Y. Weissenberger ◽  
A. J. King ◽  
J. C. Dahmen
Keyword(s):  
Inferior Colliculus ◽  
Calcium Imaging ◽  
Two Photon

Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame

2013 ◽  
Vol 110 (1) ◽  
pp. 243-256 ◽  
Author(s):  
Jakub Tomek ◽  
Ondrej Novak ◽  
Josef Syka
Keyword(s):  
Calcium Imaging ◽  
Software Package ◽  
Motion Artifacts ◽  
Calcium Signal ◽  
Neural Cells ◽  
Neuronal System ◽  
Process Data ◽  
Entire Area ◽  
Two Photon

Two-Photon Processor (TPP) is a versatile, ready-to-use, and freely available software package in MATLAB to process data from in vivo two-photon calcium imaging. TPP includes routines to search for cell bodies in full-frame (Search for Neural Cells Accelerated; SeNeCA) and line-scan acquisition, routines for calcium signal calculations, filtering, spike-mining, and routines to construct parametric fields. Searching for somata in artificial in vivo data, our algorithm achieved better performance than human annotators. SeNeCA copes well with uneven background brightness and in-plane motion artifacts, the major problems in simple segmentation methods. In the fast mode, artificial in vivo images with a resolution of 256 × 256 pixels containing ∼100 neurons can be processed at a rate up to 175 frames per second (tested on Intel i7, 8 threads, magnetic hard disk drive). This speed of a segmentation algorithm could bring new possibilities into the field of in vivo optophysiology. With such a short latency (down to 5–6 ms on an ordinary personal computer) and using some contemporary optogenetic tools, it will allow experiments in which a control program can continuously evaluate the occurrence of a particular spatial pattern of activity (a possible correlate of memory or cognition) and subsequently inhibit/stimulate the entire area of the circuit or inhibit/stimulate a different part of the neuronal system. TPP will be freely available on our public web site. Similar all-in-one and freely available software has not yet been published.

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