Digital Holography for recovering 3D shape of Red Blood Cells

The possibility to investigate cells in microfluidic flow by using a full 3D morphometry analysis is highly demanded to achieve information about their healthiness. Recently, the tomographic flow cytometry by digital holography has been demonstrated to monitor red blood cells in microfluidics environment by simply applying flux pressure to induce random self-rotation of flowing cells. Here, we provide a microfluidic solution to engineer the flow with the aim to ensure the full 360 degree of angle rotation of all cells in the field of view. We test the proposed methods for circulating tumour cells.

Download Full-text

The Talbot effect in self-assembled red blood cells investigated by digital holography

Journal of Physics Photonics ◽

10.1088/2515-7647/ab9098 ◽

2020 ◽

Vol 2 (3) ◽

pp. 035005

Author(s):

Pasquale Memmolo ◽

Lisa Miccio ◽

Francesco Merola ◽

Pietro Ferraro

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Digital Holography ◽

Talbot Effect ◽

Self Assembled

Download Full-text

3D morphometry of red blood cells by digital holography

Cytometry Part A ◽

10.1002/cyto.a.22570 ◽

2014 ◽

Vol 85 (12) ◽

pp. 1030-1036 ◽

Cited By ~ 68

Author(s):

Pasquale Memmolo ◽

Lisa Miccio ◽

Francesco Merola ◽

Oriella Gennari ◽

Paolo Antonio Netti ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Digital Holography

Download Full-text

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083266 ◽

1978 ◽

Vol 36 (2) ◽

pp. 480-481

Author(s):

Kosuke Ueda ◽

Hiroto Washida ◽

Nakazo Watari

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Renal Cortex ◽

Uranyl Acetate ◽

Osmic Acid ◽

Renal Lithiasis ◽

Electron Microscopic ◽

Hemoglobin C ◽

First Case ◽

Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Download Full-text

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072083 ◽

1973 ◽

Vol 31 ◽

pp. 328-329

Author(s):

John A. Trotter

Keyword(s):

Red Blood Cells ◽

Analytical Method ◽

Blood Cells ◽

Guinea Pigs ◽

Specific Protein ◽

Visual Examination ◽

Hemoglobin Synthesis ◽

Peroxidatic Activity ◽

Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Download Full-text