Towards laser-based open-path hydrogen sulfide detection

Author(s):  
Michal P. Nikodem
Keyword(s):  
2016 ◽  
Vol 12 (6) ◽  
pp. 465-468 ◽  
Author(s):  
Li-mei Song ◽  
Li-wen Liu ◽  
Yan-gang Yang ◽  
Qing-hua Guo ◽  
Jiang-tao Xi

2016 ◽  
Author(s):  
Michal Nikodem ◽  
Dorota Stachowiak ◽  
Piotr Jaworski

2019 ◽  
Vol 133 (20) ◽  
pp. 2045-2059 ◽  
Author(s):  
Da Zhang ◽  
Xiuli Wang ◽  
Siyao Chen ◽  
Selena Chen ◽  
Wen Yu ◽  
...  

Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, the pathogenesis of PAEC inflammation remains unclear. Methods: Purified recombinant human inhibitor of κB kinase subunit β (IKKβ) protein, human PAECs and monocrotaline-induced pulmonary hypertensive rats were employed in the study. Site-directed mutagenesis, gene knockdown or overexpression were conducted to manipulate the expression or activity of a target protein. Results: We showed that hydrogen sulfide (H2S) inhibited IKKβ activation in the cell model of human PAEC inflammation induced by monocrotaline pyrrole-stimulation or knockdown of cystathionine γ-lyase (CSE), an H2S generating enzyme. Mechanistically, H2S was proved to inhibit IKKβ activity directly via sulfhydrating IKKβ at cysteinyl residue 179 (C179) in purified recombinant IKKβ protein in vitro, whereas thiol reductant dithiothreitol (DTT) reversed H2S-induced IKKβ inactivation. Furthermore, to demonstrate the significance of IKKβ sulfhydration by H2S in the development of PAEC inflammation, we mutated C179 to serine (C179S) in IKKβ. In purified IKKβ protein, C179S mutation of IKKβ abolished H2S-induced IKKβ sulfhydration and the subsequent IKKβ inactivation. In human PAECs, C179S mutation of IKKβ blocked H2S-inhibited IKKβ activation and PAEC inflammatory response. In pulmonary hypertensive rats, C179S mutation of IKKβ abolished the inhibitory effect of H2S on IKKβ activation and pulmonary vascular inflammation and remodeling. Conclusion: Collectively, our in vivo and in vitro findings demonstrated, for the first time, that endogenous H2S directly inactivated IKKβ via sulfhydrating IKKβ at Cys179 to inhibit nuclear factor-κB (NF-κB) pathway activation and thereby control PAEC inflammation in PAH.


2019 ◽  
Vol 9 (1) ◽  
pp. 2-11
Author(s):  
Marina Efthymiou ◽  
Frank Fichert ◽  
Olaf Lantzsch

Abstract. The paper examines the workload perceived by air traffic control officers (ATCOs) and pilots during continuous descent operations (CDOs), applying closed- and open-path procedures. CDOs reduce fuel consumption and noise emissions. Therefore, they are supported by airports as well as airlines. However, their use often depends on pilots asking for CDOs and controllers giving approval and directions. An adapted NASA Total Load Index (TLX) was used to measure the workload perception of ATCOs and pilots when applying CDOs at selected European airports. The main finding is that ATCOs’ workload increased when giving both closed- and open-path CDOs, which may have a negative impact on their willingness to apply CDOs. The main problem reported by pilots was insufficient distance-to-go information provided by ATCOs. The workload change is important when considering the use of CDOs.


Author(s):  
Roberto González-De Zayas ◽  
Liosban Lantigua Ponce de León ◽  
Liezel Guerra Rodríguez ◽  
Felipe Matos Pupo ◽  
Leslie Hernández-Fernández

The Cenote Jennifer is an important and unique aquatic sinkhole in Cayo Coco (Jardines del Rey Tourist Destination) that has brackish to saline water. Two samplings were made in 1998 and 2009, and 4 metabolism community experiments in 2009. Some limnological parameters were measured in both samplings (temperature, salinity, pH, dissolved oxygen major ions, hydrogen sulfide, nutrients and others). Community metabolism was measured through incubated oxygen concentration in clear and dark oxygen bottles. Results showed that the sinkhole limnology depends on rainfall and light incidence year, with some stratification episodes, due to halocline or oxycline presence, rather than thermocline. The sinkhole water was oligotrophic (total nitrogen of 41.5 ± 22.2 μmol l−1 and total phosphorus of 0.3 ± 0.2 μmol l−1) and with low productivity (gross primary productivity of 63.0 mg C m−2 d−1). Anoxia and hypoxia were present at the bottom with higher levels of hydrogen sulfide, lower pH and restricted influence of the adjacent sea (2 km away). To protect the Cenote Jennifer, tourist exploitation should be avoided and more resources to ecological and morphological studies should be allocated, and eventually use this aquatic system only for specialized diving. For conservation purposes, illegal garbage disposal in the surrounding forest should end.


2015 ◽  
Vol 19 (0) ◽  
pp. 39-45
Author(s):  
Han-Soo Kim ◽  
◽  
Jung-Hyun Jun ◽  
Sun-Tae Kim

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