scholarly journals Extended field of view of light-sheet fluorescence microscopy by scanning multiple focus-shifted Gaussian beam arrays

2021 ◽  
Vol 29 (4) ◽  
pp. 6158
Author(s):  
Chao Liu ◽  
Chen Bai ◽  
Xianghua Yu ◽  
Shaohui Yan ◽  
Yuan Zhou ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Gaussian Beam ◽  
Light Sheet ◽  
Field Of View ◽  
Multiple Focus ◽  
Extended Field ◽  
Light Sheet Fluorescence Microscopy ◽  
Extended Field Of View

Enlarged field of view based on Schwartz modulation for light sheet fluorescence microscopy in deep tissue

2020 ◽  
Vol 45 (17) ◽  
pp. 4851
Author(s):  
Xiaobin Xu ◽  
Jiajia Chen ◽  
Biwei Zhang ◽  
Limeng Huang ◽  
Yao Zheng ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Light Sheet ◽  
Field Of View ◽  
Deep Tissue ◽  
Light Sheet Fluorescence Microscopy

Lattice light-sheet microscopy with incoherent detection and extended field of view

2021 ◽  
Author(s):  
Mariana C. Potcoava ◽  
Christopher J. Mann ◽  
Simon T. Alford ◽  
Jonathan Art
Keyword(s):  
Light Sheet ◽  
Field Of View ◽  
Light Sheet Microscopy ◽  
Extended Field ◽  
Extended Field Of View

scholarly journals Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting

2014 ◽  
Vol 11 (93) ◽  
pp. 20130851 ◽  
Author(s):  
Liang Gao ◽  
Liren Zhu ◽  
Chiye Li ◽  
Lihong V. Wang
Keyword(s):  
Fluorescence Microscopy ◽  
Biological Samples ◽  
Scattered Light ◽  
Light Sheet ◽  
Fluorescence Decay ◽  
Field Of View ◽  
Optical Section ◽  
Ballistic Regime ◽  
Application Field ◽  
Light Sheet Fluorescence Microscopy

We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section. Furthermore, the scattered-light-induced background is significantly reduced, considerably improving image contrast. Our method is expected to expand the application field of LSFM into the optical quasi-ballistic regime, enabling studies on non-transparent biological samples.

Extended Field Of View (EFOV) in Gynäkologie und Geburtshilfe

2004 ◽  
Vol 207 (S 2) ◽  
Author(s):  
W Henrich ◽  
JW Dudenhausen
Keyword(s):  
Field Of View ◽  
Extended Field ◽  
Extended Field Of View

scholarly journals Cytotoxic effects and tolerability of gemcitabine and axitinib in a xenograft model for c-myc amplified medulloblastoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stefanie Schwinn ◽  
Zeinab Mokhtari ◽  
Sina Thusek ◽  
Theresa Schneider ◽  
Anna-Leena Sirén ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Tumor Growth ◽  
Intensive Therapy ◽  
Xenograft Model ◽  
Light Sheet ◽  
Tumor Control ◽  
Orthotopic Model ◽  
Group 3 ◽  
Light Sheet Fluorescence Microscopy

AbstractMedulloblastoma is the most common high-grade brain tumor in childhood. Medulloblastomas with c-myc amplification, classified as group 3, are the most aggressive among the four disease subtypes resulting in a 5-year overall survival of just above 50%. Despite current intensive therapy regimens, patients suffering from group 3 medulloblastoma urgently require new therapeutic options. Using a recently established c-myc amplified human medulloblastoma cell line, we performed an in-vitro-drug screen with single and combinatorial drugs that are either already clinically approved or agents in the advanced stage of clinical development. Candidate drugs were identified in vitro and then evaluated in vivo. Tumor growth was closely monitored by BLI. Vessel development was assessed by 3D light-sheet-fluorescence-microscopy. We identified the combination of gemcitabine and axitinib to be highly cytotoxic, requiring only low picomolar concentrations when used in combination. In the orthotopic model, gemcitabine and axitinib showed efficacy in terms of tumor control and survival. In both models, gemcitabine and axitinib were better tolerated than the standard regimen comprising of cisplatin and etoposide phosphate. 3D light-sheet-fluorescence-microscopy of intact tumors revealed thinning and rarefication of tumor vessels, providing one explanation for reduced tumor growth. Thus, the combination of the two drugs gemcitabine and axitinib has favorable effects on preventing tumor progression in an orthotopic group 3 medulloblastoma xenograft model while exhibiting a favorable toxicity profile. The combination merits further exploration as a new approach to treat high-risk group 3 medulloblastoma.

scholarly journals Evaluation of novel AI‐based extended field‐of‐view CT reconstructions

2021 ◽  
Author(s):  
Gabriel Paiva Fonseca ◽  
Matthias Baer‐Beck ◽  
Eric Fournie ◽  
Christian Hofmann ◽  
Ilaria Rinaldi ◽  
...  
Keyword(s):  
Field Of View ◽  
Extended Field ◽  
Extended Field Of View

Visualizing three-dimensional fungal growth using light sheet fluorescence microscopy

2021 ◽  
pp. 103549
Author(s):  
Braulio Gutiérrez–Medina ◽  
Alexis Vázquez-Villa
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Fungal Growth ◽  
Light Sheet ◽  
Light Sheet Fluorescence Microscopy

Investigating safe excitation in Light Sheet Fluorescence Microscopy

2021 ◽  
Vol 84 ◽  
pp. 296
Author(s):  
Gideon Oluniran ◽  
James Blackwell ◽  
Emmanuel Reynaud ◽  
Marcin Krasny ◽  
Niall Colgan ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Light Sheet ◽  
Light Sheet Fluorescence Microscopy
Sign in / Sign up

Export Citation Format

Share Document