Single-exposure multi-wavelength diffraction imaging with a blazed grating

Multi-wavelength (mw) Bragg coherent X-ray diffraction imaging (BCDI) is demonstrated on a single Au particle. The multi-wavelength Bragg diffraction patterns are inverted using conventional phase-retrieval algorithms where the dilation of the effective pixel size of a pixelated 2D detector caused by the variation of the X-ray beam energy is mitigated by interpolating the raw data. The reconstructed Bragg electron density and phase field are in excellent agreement with the results obtained from conventional rocking scans of the same particle. Voxel sizes of about 63 nm3 are obtained for reconstructions from both approaches. Phase shifts as small as 0.41 rad, which correspond to displacements of 14 pm and translate into strain resolution better than 10−4 in the Au particle, are resolved. The displacement field changes shape during the experiment, which is well reproduced by finite element method simulations considering an inhomogeneous strained carbon layer deposited on the Au particle over the course of the measurements. These experiments thus demonstrate the very high sensitivity of BCDI and mw-BCDI to strain induced by contaminations. Furthermore, mw-BCDI offers new opportunities for in situ and operando 3D strain imaging in complex sample environments.

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Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128961 ◽

1987 ◽

Vol 45 ◽

pp. 936-937

Author(s):

Neng-Yu Zhang ◽

Terence Wagenknecht ◽

Michael Radermacher ◽

Tom Obrig ◽

Joachim Frank

Keyword(s):

Three Dimensional ◽

Ribosomal Subunit ◽

Uranyl Acetate ◽

Reconstruction Method ◽

Single Exposure ◽

Electron Dose ◽

Ribosomal Subunits ◽

40S Ribosomal Subunit ◽

Dimensional Reconstruction ◽

Rabbit Reticulocytes

We have reconstructed the 40S ribosomal subunit at a resolution of 4 nm using the single-exposure pseudo-conical reconstruction method of Radermacher et al.Small (40S) ribosomal subunits were Isolated from rabbit reticulocytes, applied to grids and negatively stained (0.5% uranyl acetate) in a manner that “sandwiches” the specimen between two layers of carbon. Regions of the grid exhibiting uniform and thick staining were identified and photographed twice (magnification 49,000X). The first micrograph was always taken with the specimen tilted by 50° and the second was of the Identical area untilted (Fig. 1). For each of the micrographs the specimen was subjected to an electron dose of 2000-3000 el/nm2.Three hundred thirty particles appearing in the L view (defined in [4]) were selected from both tilted- and untilted-specimen micrographs. The untilted particles were aligned and their rotational alignment produced the azimuthal angles of the tilted particles in the conical tilt series.

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Development of a novel apparatus for experiments in soft X-ray diffraction imaging and diffraction tomography

Journal de Physique IV (Proceedings) ◽

10.1051/jp4:200300022 ◽

2003 ◽

Vol 104 ◽

pp. 27-30 ◽

Cited By ~ 2

Author(s):

T. Beetz ◽

C. Jacobsen ◽

C.-C. Kao ◽

J. Kirz ◽

O. Mentes ◽

...

Keyword(s):

Diffraction Tomography ◽

X Ray Diffraction ◽

X Ray ◽

Diffraction Imaging

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Further Evidence that Glycoprotein IIb-IIIa Mediates Platelet Spreading on Subendothelium

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647484 ◽

1991 ◽

Vol 65 (02) ◽

pp. 202-205 ◽

Cited By ~ 37

Author(s):

Harvey J Weiss ◽

Vincet T Turitto ◽

Hans R Baumgartner

Keyword(s):

Platelet Adhesion ◽

Surface Coverage ◽

Rabbit Aorta ◽

Conclusive Evidence ◽

Initial Contact ◽

Single Exposure ◽

Vessel Segment ◽

Multiple Exposures ◽

Wall Interaction ◽

Platelet Spreading

SummaryIn order to explore further the mechanism by which glycoprotein GPIIb-IIIa promotes platelet vessel wall interaction, platelet adhesion to subendothelium was studied in an annular chamber in which subendothelium from rabbit aorta was exposed at a shear rate of 2,600 s−1 to blood from patients with thrombasthenia. Perfusions were conducted for each of 5 exposure times (1 ,2,3, 5 and 10 min), and the percent surface coverage of the vessel segment with platelets in the contact (C) and spread (S) stage was determined. Increased values of platelet contact (C) were obtained in thrombasthenia at all exposure times; this finding is consistent with a defect in platelet spreadirg, based on a previously described kinetic model of platelet attachment to subendothelium. According to this model of attachment, increased values of platelet contact (C) at a single exposure time may be indicative of either a defect in spreading (S) or initial contact (C), but multiple exposures will result in increased contact only for defects which are related to defectiye platelet spreading (s).The results obtained over a broad range of exposure times provide more conclusive evidence that GPIIb-IIIa mediates platelet spreading than those previously obtained at single exposure times.

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High Resolution Diffraction Imaging of Small Scale Fractures in Shale and Carbonate Reservoirs

Proceedings of the 3rd Unconventional Resources Technology Conference ◽

10.15530/urtec-2015-2153238 ◽

2015 ◽

Author(s):

Alexander M. Popovici ◽

Ioan Sturzu ◽

Tijmen J. Moser

Keyword(s):

High Resolution ◽

Carbonate Reservoirs ◽

Small Scale ◽

Diffraction Imaging

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Fabrication of two-layer integrated phase mask and single-beam, single exposure fabrication of 3D photonic crystal template using the mask

10.2351/1.5061421 ◽

2008 ◽

Author(s):

Yuankun Lin ◽

Ahmad Harb ◽

Daniel Rodriguez ◽

Karen Lozano ◽

Di Xu ◽

...

Keyword(s):

Photonic Crystal ◽

Phase Mask ◽

Single Exposure ◽

Single Beam

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An Over 120dB Dynamic Range Linear Response Single Exposure CMOS Image Sensor with Two-stage Lateral Overflow Integration Trench Capacitors

Electronic Imaging ◽

10.2352/issn.2470-1173.2020.7.iss-143 ◽

2020 ◽

Vol 2020 (7) ◽

pp. 143-1-143-6 ◽

Cited By ~ 1

Author(s):

Yasuyuki Fujihara ◽

Maasa Murata ◽

Shota Nakayama ◽

Rihito Kuroda ◽

Shigetoshi Sugawa

Keyword(s):

Linear Response ◽

Dynamic Range ◽

Signal To Noise Ratio ◽

Cmos Image Sensor ◽

Image Sensor ◽

Signal To Noise ◽

Single Exposure ◽

Two Stage ◽

Noise Ratio ◽

Maximum Signal

This paper presents a prototype linear response single exposure CMOS image sensor with two-stage lateral overflow integration trench capacitors (LOFITreCs) exhibiting over 120dB dynamic range with 11.4Me- full well capacity (FWC) and maximum signal-to-noise ratio (SNR) of 70dB. The measured SNR at all switching points were over 35dB thanks to the proposed two-stage LOFITreCs.

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