STUDY OF SIGNIFICANCE OF PHASE MASK IMAGE IN ACUTE STROKE PATIENTS

Background: Phase images contains information regarding local susceptibility changes between the tissues, which can help measure the iron and other content which changes the local field. Typically, this information is ignored before looking at console. Susceptibility weighted imaging (SWI) is a magnetic resonance (MR) technique detects an early hemorrhagic transformation within the infarct to provide insight into cerebral hemodynamics following the stroke. Objective: Significance of “phase mask imaging in differentiation of hemorrhage and calcifications” in acute stroke patients. Methods: An observational non-interventional study carried out on 100 patients with stroke and headache symptoms. MRI Brain Stroke Profile with FLAIR, DWI, ADC, SWAN, and Phase mask sequences, done on 3T GE MRI scanner. Results: All patients underwent MRI study with SWI sequence. Of 183 cases, 33%(n=60) patients had microbleeds, 5%(n=10) patients had granulomas, 32%(n=58) patients had arterial thrombus with infarct, 11%(n=20) patients had falx calcifications, 11%(n=20) patients had intraparenchymal haemorrhage, and 8%(n=15) patients had infarcts with haemorrhagic transformation. The sensitivity of phase imaging in the detection of calcification was 90%. Conclusion: Phase mask imaging plays an important role to detect intracranial calcifications and chronic microbleeds. Phase mask imaging acts as a supplement tool in acute stroke patients, which guides further management.

Adaptive Wave-Front Shaping and Beam Focusing through Fiber Bundles for High-Resolution Bioimaging

We demonstrate an adaptive wave-front shaping of optical beams transmitted through fiber bundles as a powerful resource for multisite, high-resolution bioimaging. With the phases of all the beamlets delivered through up to 6000 different fibers within the fiber bundle controlled individually, by means of a high-definition spatial light modulator, the overall beam transmitted through the fiber bundle can be focused into a beam waist with a diameter less than 1 μm within a targeted area in a biotissue, providing a diffraction-limited spatial resolution adequate for single-cell or even subcellular bioimaging. The field intensity in the adaptively-focused continuous-wave laser beam in our fiber-bundle-imaging setting is more than two orders of magnitude higher than the intensity of the speckle background. Once robust beam focusing was achieved with a suitable phase profile across the input face of the fiber bundle, the beam focus can be scanned over a targeted area with no need for a further adaptive search, by applying a physically intuitive, wave-front-tilting phase mask on the field of input beamlets. This method of beam-focus scanning promises imaging speeds compatible with the requirements of in vivo calcium imaging.

pixOL: pixel-wise dipole-spread function engineering for simultaneously measuring the 3D orientation and 3D localization of dipole-like emitters

Interactions between biomolecules are characterized by both where they occur and how they are organized, e.g., the alignment of lipid molecules to form a membrane. However, spatial and angular information are mixed within the image of a fluorescent molecule-the microscopy's dipole spread function (DSF). We demonstrate the pixOL algorithm for simultaneously optimizing all pixels within a phase mask to produce an engineered Green's tensor-the dipole extension of point-spread function engineering. The pixOL DSF achieves optimal precision for measuring simultaneously the 3D orientation and 3D location of a single molecule, i.e., 1.14 degree orientation, 0.24 sr wobble angle, 8.17 nm lateral localization, and 12.21 nm axial localization precisions over an 800-nm depth range using 2500 detected photons. The pixOL microscope accurately and precisely resolves the 3D positions and 3D orientations of Nile red within a spherical supported lipid bilayer, resolving both membrane defects and differences in cholesterol concentration, in 6 dimensions.

The Effect of the Range of a Modulating Phase Mask on the Retrieval of a Complex Object from Intensity Measurements

In many fields of science, it is often impossible to preserve the information about the phase of the electromagnetic field, and only the information about the magnitude is available. This is known as the phase problem. Various algorithms have been proposed to recover the information about phase from intensity measurements. Nowadays, iterative algorithms of phase retrieval have become popular. Many of these algorithms are based on modulating the object under study with several masks and retrieving the missing information about the phase of an object by applying mathematical optimization methods. Several of these algorithms are able to retrieve not only the phase but also the magnitude of the object under study. In this study, we investigate the effect of the range of modulation of a mask on the accuracy of the retrieved magnitude and phase map. We conclude that there is a sharp boundary of the range of modulation separating the successfully retrieved magnitude and phase maps from those retrieved unsuccessfully. A decrease in the range of modulation affects the accuracy of the retrieved magnitude and phase map differently.

Asymmetric optical cryptosystem for multiple images based on devil’s spiral Fresnel lens phase and random spiral transform in gyrator domain

An asymmetric cryptosystem is presented for encrypting multiple images in gyrator transform domains. In the encryption approach, the devil’s spiral Fresnel lens variable pure phase mask is first designed for each image band to be encrypted by using devil’ mask, random spiral phase and Fresnel mask, respectively. Subsequently, a novel random devil’ spiral Fresnel transform in optical gyrator transform is implemented to achieved the intermediate output. Then, the intermediate data is divided into two masks by employing random modulus decomposition in the asymmetric process. Finally, a random permutation matrix is utilized to obtain the ciphertext of the intact algorithm. For the decryption approach, two divided masks (private key and ciphertext) need to be imported into the optical gyrator input plane simultaneously. Some numerical experiments are given to verify the effectiveness and capability of this asymmetric cryptosystem.