Optical clearing agent for enhanced imaging dorsal blood vessels in nude mice

AbstractThe results of a feasibility study of the application of PEG-300 and fructose as two independent optical clearing agents for the reduction of light scattering in biological tissues are presented.An OCT system operating at 1300 nm was used to study optical clearing effects. InThe intradermal injection of fructose in combination with the intravenous injection of PEG-300 led to a rapid optical clearing effect. In the experiments on miceThe experiments on mice have clearly demonstrated that intradermal and intravenous injections of optical clearing agents enhanced light transport through the skin and blood vessels.

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Short-term and long-term effects of optical clearing agents on blood vessels in chick chorioallantoic membrane

Journal of Biomedical Optics ◽

10.1117/1.2907169 ◽

2008 ◽

Vol 13 (2) ◽

pp. 021106 ◽

Cited By ~ 29

Author(s):

Dan Zhu ◽

Jing Zhang ◽

Han Cui ◽

Zongzhen Mao ◽

Pengcheng Li ◽

...

Keyword(s):

Blood Vessels ◽

Chorioallantoic Membrane ◽

Long Term Effects ◽

Short Term ◽

Optical Clearing ◽

Chick Chorioallantoic Membrane

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Abnormal blood vessels formed by human liver cavernous hemangioma endothelial cells in nude mice are suitable for drug evaluation

Microvascular Research ◽

10.1016/j.mvr.2009.08.007 ◽

2009 ◽

Vol 78 (3) ◽

pp. 379-385 ◽

Cited By ~ 3

Author(s):

Wen-jian Zhang ◽

Lian-qiu Wu ◽

Hong-lin Liu ◽

Li-ya Ye ◽

Yu-ling Xin ◽

...

Keyword(s):

Endothelial Cells ◽

Blood Vessels ◽

Nude Mice ◽

Cavernous Hemangioma ◽

Human Liver ◽

Drug Evaluation ◽

Liver Cavernous Hemangioma

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Rapid skin optical clearing enhancement with salicylic acid for imaging blood vessels in vivo

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2020.102005 ◽

2020 ◽

Vol 32 ◽

pp. 102005

Author(s):

Huabin He ◽

Yi Shen ◽

Buhong Li

Keyword(s):

Salicylic Acid ◽

Blood Vessels ◽

Optical Clearing

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Ear skin optical clearing for improving blood flow imaging/Optisches Clearing der Ohrhaut zur verbesserten Bildgebung des Blutflusses

Photonics and Lasers in Medicine ◽

10.1515/plm-2012-0055 ◽

2013 ◽

Vol 2 (1) ◽

Cited By ~ 5

Author(s):

Jing Wang ◽

Rui Shi ◽

Yang Zhang ◽

Dan Zhu

Keyword(s):

Blood Flow ◽

Blood Vessels ◽

Imaging Techniques ◽

Flow Distribution ◽

Optical Methods ◽

Optical Clearing ◽

Mouse Ear ◽

Tumor Studies ◽

Imaging Depth ◽

Cutaneous Blood

AbstractVarious optical imaging techniques have shown a great potential for monitoring angiogenesis, development of blood vessels, and even tumor transfer, but they suffer from the limited imaging depth in tissue. Although the mouse ear provides an available window, the residual scattering of ear skin still influences the imaging quality. The proposed tissue optical clearing technique presents a new opportunity to decrease the scattering of skin, and enhance the imaging contrast or imaging depth of optical methods. The purpose of this study is to develop an innovative ear skin optical clearing agent (ESOCA) for improving the transparency of the mouse ear.The ESOCA was topically applied on the ear skin of BALB/c miceThe transmittance of mice ear was enhanced by 111.0±8.2% at 633 nm after application of ESOCA. The cutaneous blood vessels and blood flow could be distinguished more clearly with LSCI technique. In addition, the calculated CNR values of speckle contrast images showed a great enhancement compared with the initial (control) values.In summary, topical application of an innovative ESOCA permits the vessel structure and flow distribution information of cutaneous blood vessels to be imaged by LSCI with higher contrast, which will be significant for tumor studies in the future.

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Abstract 5553: Real-time imaging of αvintegrin-GFP expression in osteosarcoma cells trafficking in blood vessels of nude mice

10.1158/1538-7445.am2012-5553 ◽

2012 ◽

Author(s):

Yasunori Tome ◽

Naotoshi Sugimoto ◽

Shuya Yano ◽

Masashi Momiyama ◽

Hiroki Maehara ◽

...

Keyword(s):

Blood Vessels ◽

Real Time ◽

Nude Mice ◽

Osteosarcoma Cells ◽

Gfp Expression ◽

Real Time Imaging

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Immunohistochemical and Morphometric Characterization of Blood Vessels in Brain Metastasis in Nude Mice

Microscopy and Microanalysis ◽

10.1017/s1431927600015804 ◽

1999 ◽

Vol 5 (S2) ◽

pp. 496-497

Author(s):

C. D. Bucana ◽

S. Yano ◽

D. Reynolds ◽

K. Dunner ◽

I. J. Fidler

Keyword(s):

Brain Metastasis ◽

Blood Vessels ◽

Nude Mice ◽

Cellular Localization ◽

Brain Parenchyma ◽

Adult Brain ◽

Host Cells ◽

Leukocyte Antigens ◽

Morphometric Characterization

This study was conducted to compare the immunohistochemical and morphometric characteristics of blood vessels in brain metastasis with blood vessels of the adjacent uninvolved brain tissue. This is based on the hypothesis that new vessels formed in the tumor express antigens that are not expressed in blood vessels of the adult brain parenchyma. Thus, a panel of antibodies known to react with mouse endothelial cells at various stages of differentiation or stimulation was tested on frozen sections of experimental brain metastasis. Intratumoral microvessel density which has been reported to reflect the intensity of tumor angiogenesis was inapplicable to experimental brain metastasis therefore other parameters were measured.Brain metastasis was produced in nude mice following intracarotid injection of tumor cells. Three human tumors and three murine tumors were injected into the carotid artery of nude mice. The animals were sacrificed when they were moribund, the brains were embedded in OCT and frozen in liquid nitrogen. Some animals were injected with bromodeoxyuridine (BrdU) 1 h before they were necropsied. Immunohistochemistry was performed using antibodies directed to the following antigens: CD31, CD34, MECA-32, endoglin, ICAM-1, ICAM-2, and BrdU. Antibodies to leukocyte antigens, F4/80 and CD3 were also tested to determine the amount of host cells infiltrating the tumors. SEM of immunogold labeled preparations determined the cellular localization of some antigens.

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