Altered levels of memory T cell subsets and common γc cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment

ABSTRACT Strongyloides stercoralis infection is associated with diminished antigen-specific Th1- and Th17-associated responses and enhanced Th2-associated responses. Interleukin-27 (IL-27) and IL-37 are two known anti-inflammatory cytokines that are highly expressed in S. stercoralis infection. We therefore wanted to examine the role of IL-27 and IL-37 in regulating CD4+ and CD8+ T cell responses in S. stercoralis infection. To this end, we examined the frequency of Th1/Tc1, Th2/Tc2, Th9/Tc9, Th17/Tc17, and Th22/Tc22 cells in 15 S. stercoralis-infected individuals and 10 uninfected individuals stimulated with parasite antigen following IL-27 or IL-37 neutralization. We also examined the production of prototypical type 1, type 2, type 9, type 17, and type 22 cytokines in the whole-blood supernatants. Our data reveal that IL-27 or IL-37 neutralization resulted in significantly enhanced frequencies of Th1/Tc1, Th2/Tc2, Th17/Tc17, Th9, and Th22 cells with parasite antigen stimulation. There was no induction of any T cell response in uninfected individuals following parasite antigen stimulation and IL-27 or IL-37 neutralization. Moreover, we also observed increased production of gamma interferon (IFN-γ), IL-5, IL-9, IL-17, and IL-22 and decreased production of IL-10 following IL-27 and IL-37 neutralization and parasite antigen stimulation in whole-blood cultures. Thus, we demonstrate that IL-27 and IL-37 limit the induction of particular T cell subsets along with cytokine responses in S. stercoralis infections, which suggest the importance of IL-27 and IL-37 in immune modulation in a chronic helminth infection.

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Comparison of selected CD45+ cell subsets’ response and cytokine levels on exhaustive effort among soccer players

Journal of Medical Biochemistry ◽

10.2478/jomb-2018-0029 ◽

2019 ◽

Vol 38 (3) ◽

pp. 256-267 ◽

Cited By ~ 6

Author(s):

Dorota Kostrzewa-Nowak ◽

Rafał Buryta ◽

Robert Nowak

Keyword(s):

T Cell ◽

Biological Response ◽

T Cell Subsets ◽

Alternative Mechanism ◽

Post Exercise ◽

Memory T Cell ◽

Cell Percentage ◽

Significant Release ◽

Efficiency Test ◽

The Impact

SummaryBackgroundImmunological alterations may led to the reduction in capacity and endurance levels in elite athletes by e.g. increased susceptibility to infections. There is a need to explain the impact of intensive physical effort on the CD4+memory T cell subsets.MethodsFourteen participants median aged 19 years old (range 17–21 years) were recruited form Pogoń Szczecin S.A., soccer club. They performed progressive efficiency test on mechanical treadmill until exhaustion twice: during preparatory phases to spring and autumn competition rounds. We examined the influence of exhaustive effort on the selected CD45+, especially CD4+memory T cell subsets and inflammation markers determined before, just after the test and during recovery time.ResultsSignificant changes in total CD45+cells and decrease in T lymphocytes percentage after the run was observed. Significant fluctuations in T cells’ distribution were related not only to the changes in Th or Tc subsets but also to increase in naïve T cell percentage during recovery. Increase in TNF-α and IL-8 post-exercise, IL-6 and IL-10 plasma levels in recovery was also found.ConclusionsThe novel finding of our study is that the run performed on mechanical treadmill caused a significant release of CD4+T naïve cells into circulation. Post-exercise increase in circulating NK cells is related with fast biological response to maximal effort. However, at the same time an alternative mechanism enhancing inflammation is involved.

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201. Higher levels of memory T-cell subsets in decidua parietalis compared to basalis tissue of uncomplicated term pregnancies

Pregnancy Hypertension ◽

10.1016/j.preghy.2018.08.288 ◽

2018 ◽

Vol 13 ◽

pp. S97

Author(s):

Anne Laskewitz ◽

Lisanne Zijlker ◽

Annege Vledder ◽

Sicco Scherjon ◽

Marijke Faas ◽

...

Keyword(s):

T Cell ◽

T Cell Subsets ◽

Memory T Cell

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Clinical, Immunological and Treatment-Related Factors Associated with Normalised CD4+/CD8+ T-Cell Ratio: Effect of Naïve and Memory T-Cell Subsets

PLoS ONE ◽

10.1371/journal.pone.0097011 ◽

2014 ◽

Vol 9 (5) ◽

pp. e97011 ◽

Cited By ~ 32

Author(s):

Willard Tinago ◽

Elizabeth Coghlan ◽

Alan Macken ◽

Julie McAndrews ◽

Brenda Doak ◽

...

Keyword(s):

T Cell ◽

Cd8 T Cell ◽

T Cell Subsets ◽

Related Factors ◽

Cell Ratio ◽

Ratio Effect ◽

Memory T Cell ◽

Factors Associated

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MA12.10 Long-Term Impact of Radiotherapy Before Surgery for Mesothelioma on the Distribution of Memory T Cell Subsets

Journal of Thoracic Oncology ◽

10.1016/j.jtho.2018.08.419 ◽

2018 ◽

Vol 13 (10) ◽

pp. S399 ◽

Cited By ~ 1

Author(s):

J. Murakami ◽

L. Wu ◽

M. Kohno ◽

M. Chan ◽

Y. Zhao ◽

...

Keyword(s):

T Cell ◽

T Cell Subsets ◽

Memory T Cell ◽

Long Term Impact

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Impact of multiple hits with cognate antigen on memory CD8+ T-cell fate

International Immunology ◽

10.1093/intimm/dxaa039 ◽

2020 ◽

Vol 32 (9) ◽

pp. 571-581 ◽

Cited By ~ 1

Author(s):

Shiki Takamura

Keyword(s):

T Cells ◽

T Cell ◽

Cd8 T Cells ◽

Memory T Cells ◽

Cd8 T Cell ◽

T Cell Subsets ◽

Primary Immune Response ◽

Memory T Cell ◽

Cognate Antigen ◽

Memory Cd8 T Cell

Abstract Antigen-driven activation of CD8+ T cells results in the development of a robust anti-pathogen response and ultimately leads to the establishment of long-lived memory T cells. During the primary response, CD8+ T cells interact multiple times with cognate antigen on distinct types of antigen-presenting cells. The timing, location and context of these antigen encounters significantly impact the differentiation programs initiated in the cells. Moderate re-activation in the periphery promotes the establishment of the tissue-resident memory T cells that serve as sentinels at the portal of pathogen entry. Under some circumstances, moderate re-activation of T cells in the periphery can result in the excessive expansion and accumulation of circulatory memory T cells, a process called memory inflation. In contrast, excessive re-activation stimuli generally impede conventional T-cell differentiation programs and can result in T-cell exhaustion. However, these conditions can also elicit a small population of exhausted T cells with a memory-like signature and self-renewal capability that are capable of responding to immunotherapy, and restoration of functional activity. Although it is clear that antigen re-encounter during the primary immune response has a significant impact on memory T-cell development, we still do not understand the molecular details that drive these fate decisions. Here, we review our understanding of how antigen encounters and re-activation events impact the array of memory CD8+ T-cell subsets subsequently generated. Identification of the molecular programs that drive memory T-cell generation will advance the development of new vaccine strategies that elicit high-quality CD8+ T-cell memory.

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