As various new sibling species within theScedosporiumspp. have been described recently, this study was conducted to investigate distribution and antifungal susceptibility profiles of the different species ofScedosporiumspp. in China. Twenty-one clinical strains ofScedosporiumfrom China and two strains from Japan were reidentified by MLSA. The analysis included BT2, CAL, RPB, SOD, and ACT and the combination of the five loci.Pseudallescheria boydiicomplex (17 strains) andS. apiospermum(6 strains) were identified.P. boydiicomplex included four closely related subgroups:P. boydii(9 strains),P. ellipsoidea(6 strains),P. fusoidea(1 strain), andP. angusta(1 strain). There were no significant differences in MICs for neither VOR, POS, nor AMB over all the five species in study. For itraconazole, intraspecific diversity was evident.