scholarly journals Prevalence, distribution and antifungal susceptibility profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis bloodstream isolates

2012 ◽  
Vol 61 (7) ◽  
pp. 1003-1008 ◽  
Author(s):  
Lucas Xavier Bonfietti ◽  
Marilena dos Anjos Martins ◽  
Maria Walderez Szeszs ◽  
Sandra Brasil Stolf Pukiskas ◽  
Sonia Ueda Purisco ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Susceptibility Profiles

scholarly journals Prevalence and Susceptibility Profile of Candida metapsilosis and Candida orthopsilosis: Results from Population-Based Surveillance of Candidemia in Spain

2008 ◽  
Vol 52 (4) ◽  
pp. 1506-1509 ◽  
Author(s):  
A. Gomez-Lopez ◽  
A. Alastruey-Izquierdo ◽  
D. Rodriguez ◽  
B. Almirante ◽  
A. Pahissa ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Population Based ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Susceptibility Profiles

ABSTRACT We describe the prevalences and susceptibility profiles of two recently described species, Candida metapsilosis and Candida orthopsilosis, related to Candida parapsilosis in candidemia. The prevalences of these species (1.7% for C. metapsilosis and 1.4% for C. orthopsilosis) are significant. Differences observed in their susceptibility profiles could have therapeutic importance.

scholarly journals Molecular differentiation and antifungal susceptibilities of Candida parapsilosis isolated from patients with bloodstream infections

2009 ◽  
Vol 58 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Sun Tee Tay ◽  
Shiang Ling Na ◽  
Jennifer Chong
Keyword(s):  
Candida Parapsilosis ◽  
Susceptibility Testing ◽  
Rapd Analysis ◽  
Antifungal Susceptibility ◽  
Bloodstream Infections ◽  
Candida Metapsilosis ◽  
Significant Difference ◽  
Its Gene ◽  
Candida Orthopsilosis ◽  
Susceptibility Patterns

The genetic heterogeneity and antifungal susceptibility patterns of Candida parapsilosis isolated from blood cultures of patients were investigated in this study. Randomly amplified polymorphic DNA (RAPD) analysis generated 5 unique profiles from 42 isolates. Based on the major DNA fragments of the RAPD profiles, the isolates were identified as RAPD type P1 (29 isolates), P2 (6 isolates), P3 (4 isolates), P4 (2 isolates) and P5 (1 isolate). Sequence analysis of the internal transcribed spacer (ITS) gene of the isolates identified RAPD type P1 as C. parapsilosis, P2 and P3 as Candida orthopsilosis, P4 as Candida metapsilosis, and P5 as Lodderomyces elongisporus. Nucleotide variations in ITS gene sequences of C. orthopsilosis and C. metapsilosis were detected. Antifungal susceptibility testing using Etests showed that all isolates tested in this study were susceptible to amphotericin B, fluconazole, ketoconazole, itraconazole and voriconazole. C. parapsilosis isolates exhibited higher MIC50 values than those of C. orthopsilosis for all of the drugs tested in this study; however, no significant difference in the MICs for these two Candida species was observed. The fact that C. orthopsilosis and C. metapsilosis were responsible for 23.8 and 4.8 % of the cases attributed to C. parapsilosis bloodstream infections, respectively, indicates the clinical relevance of these newly described yeasts. Further investigations of the ecological niche, mode of transmission and virulence of these species are thus essential.

scholarly journals Identification of Candida parapsilosis Species Complex and Lodderomyces elongisporus Isolates with MALDI-TOF MS

2021 ◽  
Author(s):  
Engin Kaplan ◽  
Ayşe Sultan Karakoyun ◽  
Deniz Alkaya ◽  
Nevzat Ünal ◽  
Aylin Döğen ◽  
...  
Keyword(s):  
Species Complex ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Maldi Tof Ms ◽  
Biochemical Methods ◽  
Maldi Tof ◽  
Candida Orthopsilosis ◽  
Susceptibility Profiles ◽  
Reference Strains ◽  
Tof Ms

Objective: Candida parapsilosis species complex and Lodderomyces elongisporus may have differences in terms of their virulence, prevalence, and antifungal susceptibility profiles. These species are difficult to identify with biochemical methods. Therefore, there is a need for more efficient identification methods in terms of time, cost, and applicability. This study aims to evaluate the diagnostic performance of the MALDI-TOF MS method in discriminating between isolates belonging to the C. parapsilosis species complex and L. elongisporus. Method: In the current study, a total of 32 reference strains, including the C. parapsilosis (n=8), Candida orthopsilosis (n=7), Candida metapsilosis (n=6), and L. elongisporus (n=11) species were identified using the MALDI-TOF MS method. Results: The species names of 31 (93.7%) isolates belonging to the C. parapsilosis species complex and L.elongisporus were correctly identified. Twenty four isolates including eight (100%) C. parapsilosis, five (83%) C. metapsilosis, five (71%) C. orthopsilosis, and six (54%) L. elongisporus isolates were identified with score values ranging from 1.7 to 2.14. According to the secure identification reference score of ≥ 1.7, the sensitivity and specificity of the MALDI-TOF MS method were determined as 54.5–100% and 96.3–100%, respectively. Conclusion: Although the MALDI-TOF MS method has been shown to be effective in the rapid molecular phenotypic diagnosis of species that were difficult to discriminate using biochemical methods such as C. parapsilosis species complex and L. elongisporus, there is a clear need to optimize the method and develop a larger MS library for species-level identification within secure score ranges.

scholarly journals Prospective Multicenter Study of the Epidemiology, Molecular Identification, and Antifungal Susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis Isolated from Patients with Candidemia

2011 ◽  
Vol 55 (12) ◽  
pp. 5590-5596 ◽  
Author(s):  
Emilia Cantón ◽  
Javier Pemán ◽  
Guillermo Quindós ◽  
Elena Eraso ◽  
Ilargi Miranda-Zapico ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Multicenter Study ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Colorimetric Method ◽  
Prospective Multicenter Study ◽  
Content Type ◽  
Adult Intensive Care Unit ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis

ABSTRACTA 13-month prospective multicenter study including 44 hospitals was carried out to evaluate the epidemiology ofCandida parapsilosiscomplex candidemia in Spain. Susceptibility to amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole, posaconazole, anidulafungin, caspofungin, and micafungin was tested by the microdilution colorimetric method. A total of 364C. parapsilosiscomplex isolates were identified by molecular methods:C. parapsilosis(90.7%),Candida orthopsilosis(8.2%), andCandida metapsilosis(1.1%). Most candidemias (C. parapsilosis, 76.4%;C. orthopsilosis, 70.0%;C. metapsilosis, 100%) were observed in adults. NoC. orthopsilosisorC. metapsilosiscandidemias occurred in neonates.C. parapsilosiswas most frequent in adult intensive care unit (28.8%), surgery (20.9%), and internal medicine (19.7%) departments; andC. orthopsilosiswas most frequent in hematology (28.6%), pediatrics (12.0%), and neonatology (11.5%) departments. The geographic distribution ofC. orthopsilosisandC. metapsilosiswas not uniform. According to CLSI clinical breakpoints, allC. orthopsilosisandC. metapsilosisisolates were susceptible to the nine agents tested. Resistance (MICs > 1 mg/liter) was observed only inC. parapsilosis: amphotericin B, posaconazole, itraconazole, and caspofungin (0.3% each), anidulafungin (1.9%), and micafungin (2.5%). Applying the new species-specific fluconazole and echinocandin breakpoints, the rates of resistance to fluconazole forC. parapsilosisandC. orthopsilosisincreased to 4.8% and 0.3%, respectively; conversely, forC. parapsilosisthey shifted from 1.9 to 0.6% (anidulafungin) and from 2.5 to 0.6% (micafungin). Our study confirms the different prevalence ofC. parapsilosiscomplex candidemia among age groups: neitherC. orthopsilosisnorC. metapsilosiswas isolated from neonates; interestingly,C. metapsilosiswas isolated only from adults and the elderly. The disparity in antifungal susceptibility among species could be important for therapy.

scholarly journals A case series of medically managed Candida parapsilosis complex prosthetic valve endocarditis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Penghao Guo ◽  
Yuting He ◽  
Rui Fan ◽  
Zhongwen Wu ◽  
Yili Chen ◽  
...  
Keyword(s):  
Prosthetic Valve ◽  
Candida Parapsilosis ◽  
Case Series ◽  
Prosthetic Valve Endocarditis ◽  
Sensu Stricto ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Tof Ms

Abstract Background In recent years, Candida parapsilosis is recognized as a species complex and is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis. Candida parapsilosis complex prosthetic valve endocarditis (PVE) is rare and the survival rate is still low despite of optimal therapeutic strategies. In our report, it is novel to report cases as Candida parapsilosis complex PVE at species and identify Candida parapsilosis using MALDI-TOF MS. Case presentation A series of 4 cases of Candida parapsilosis complex PVE from our institution was reported. Three were infected by Candida parapsilosis sensu stricto and one was infected by Candida metapsilosis. The condition of two cases got better and the other died. Conclusions More attention should be paid to Candida parapsilosis complex PVE and early diagnosis and prompt antibiotic therapy may play a role in the treatment for Candida parapsilosis complex PVE. It is recommended to identify Candida parapsilosis complex at species level and MALDI-TOF MS as an easy, fast and efficient identification method is worth promoting in clinical microbiology

Virulence of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis in reconstituted human tissue models

2007 ◽  
Vol 44 (12) ◽  
pp. 1336-1341 ◽  
Author(s):  
Attila Gácser ◽  
Wilhelm Schäfer ◽  
Jerome S. Nosanchuk ◽  
Siegfried Salomon ◽  
Joshua D. Nosanchuk
Keyword(s):  
Human Tissue ◽  
Candida Parapsilosis ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Tissue Models

scholarly journals A Naturally Occurring Proline-to-Alanine Amino Acid Change in Fks1p in Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis Accounts for Reduced Echinocandin Susceptibility

2008 ◽  
Vol 52 (7) ◽  
pp. 2305-2312 ◽  
Author(s):  
Guillermo Garcia-Effron ◽  
Santosh K. Katiyar ◽  
Steven Park ◽  
Thomas D. Edlind ◽  
David S. Perlin
Keyword(s):  
Amino Acid ◽  
Candida Parapsilosis ◽  
Hot Spot ◽  
Amino Acid Position ◽  
Intrinsic Resistance ◽  
Glucan Synthase ◽  
Equivalent Position ◽  
Naturally Occurring ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis

ABSTRACT Candida parapsilosis has emerged as a common cause of invasive fungal infection, especially in Latin America and in the neonatal setting. C. parapsilosis is part of a closely related group of organisms that includes the species Candida orthopsilosis and Candida metapsilosis. All three species show elevated MICs for the new echinocandin class drugs caspofungin, micafungin, and anidulafungin relative to other Candida species. Despite potential impacts on therapy, the mechanism behind this reduced echinocandin susceptibility has not been determined. In this report, we investigated the role of a naturally occurring Pro-to-Ala substitution at amino acid position 660 (P660A), immediately distal to the highly conserved hot spot 1 region of Fks1p, in the reduced-echinocandin-susceptibility phenotype. Kinetic inhibition studies demonstrated that glucan synthase from the C. parapsilosis group was 1 to 2 logs less sensitive to echinocandin drugs than the reference enzyme from C. albicans. Furthermore, clinical isolates of C. albicans and C. glabrata which harbor mutations at this equivalent position also showed comparable 2-log decreases in target enzyme sensitivity, which correlated with increased MICs. These mutations also resulted in 2.4- to 18.8-fold-reduced V max values relative to those for the wild-type enzyme, consistent with kinetic parameters obtained for C. parapsilosis group enzymes. Finally, the importance of the P660A substitution for intrinsic resistance was confirmed by engineering an equivalent P647A mutation into Fks1p of Saccharomyces cerevisiae. The mutant glucan synthase displayed characteristic 2-log decreases in sensitivity to the echinocandin drugs. Overall, these data firmly indicate that a naturally occurring P660A substitution in Fks1p from the C. parapsilosis group accounts for the reduced susceptibility phenotype.

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