scholarly journals Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples

2015 ◽  
Vol 48 (4) ◽  
pp. 454-459 ◽  
Author(s):  
Fábio Silvestre Ataides ◽  
Carolina Rodrigues Costa ◽  
Lúcia Kioko Hasimoto e Souza ◽  
Orionalda deFátima Lisboa Fernandes ◽  
Rosália Santos Amorim Jesuino ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Culture Collection ◽  
Clinical Samples ◽  
Complex Species ◽  
Candida Parapsilosis Complex ◽  
Susceptibility Profiles

scholarly journals Prevalence, distribution and antifungal susceptibility profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis bloodstream isolates

2012 ◽  
Vol 61 (7) ◽  
pp. 1003-1008 ◽  
Author(s):  
Lucas Xavier Bonfietti ◽  
Marilena dos Anjos Martins ◽  
Maria Walderez Szeszs ◽  
Sandra Brasil Stolf Pukiskas ◽  
Sonia Ueda Purisco ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Susceptibility Profiles

scholarly journals Rapid Detection of Echinocandins Resistance by MALDI-TOF MS in Candida parapsilosis Complex

2020 ◽  
Vol 8 (1) ◽  
pp. 109
Author(s):  
Ana Emília M. Roberto ◽  
Danilo E. Xavier ◽  
Esteban E. Vidal ◽  
Cláudia Fernanda de L. Vidal ◽  
Rejane P. Neves ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Incubation Time ◽  
Candida Parapsilosis ◽  
Clinical Laboratory ◽  
Antifungal Susceptibility ◽  
Reference Method ◽  
Sensu Stricto ◽  
Maldi Tof ◽  
Separation Parameter ◽  
Candida Parapsilosis Complex

Mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) was used to identify and differentiate the pattern of susceptibility of clinical isolates of Candida parapsilosis complex. 17 C. parapsilosis sensu stricto, 2 C. orthopsilosis, and 1 C. metapsilosis strains were obtained from blood cultures, and three different inocula (103, 105, and 107 CFU/mL) were evaluated against three echinocandins at concentrations ranging from 0.03 to 16 µg/mL after incubation of 1 h, 2 h, and 3 h. Drug-free control was used. The spectra obtained at these concentrations were applied to generate composite correlation index (CCI) matrices for each yeast individually. After cross correlations and autocorrelations of each spectra with null (zero) and maximal (16) concentrations, the CCI was used as separation parameter among spectra. Incubation time and inoculum were critical factors to reach higher precision and reliability of this trial. With an incubation time of 3 h and inoculum of 107 CFU/mL, it was possible to determine the breakpoint of the clinical yeasts by MALDI-TOF that presented high agreement with the clinical laboratory standard institute (CLSI) reference method. Herein, we show that mass spectrometry using the MALDI-TOF technique is powerful when it exploits antifungal susceptibility testing assays.

scholarly journals Diversity of Bipolaris Species in Clinical Samples in the United States and Their Antifungal Susceptibility Profiles

2012 ◽  
Vol 50 (12) ◽  
pp. 4061-4066 ◽  
Author(s):  
K. C. da Cunha ◽  
D. A. Sutton ◽  
A. W. Fothergill ◽  
J. Cano ◽  
J. Gene ◽  
...  
Keyword(s):  
United States ◽  
Antifungal Susceptibility ◽  
The United States ◽  
Clinical Samples ◽  
Susceptibility Profiles

scholarly journals Comparison of four molecular approaches to identify Candida parapsilosis complex species

2017 ◽  
Vol 112 (3) ◽  
pp. 214-219 ◽  
Author(s):  
Leonardo Silva Barbedo ◽  
Maria Helena Galdino Figueiredo-Carvalho ◽  
Mauro de Medeiros Muniz ◽  
Rosely Maria Zancopé-Oliveira
Keyword(s):  
Candida Parapsilosis ◽  
Complex Species ◽  
Molecular Approaches ◽  
Candida Parapsilosis Complex

scholarly journals An alarming rise of non-albicans Candida species and uncommon yeasts in the clinical samples; a combination of various molecular techniques for identification of etiologic agents

2019 ◽  
Author(s):  
Mohireh Taei ◽  
Mostafa Chadeganipour ◽  
Rasoul Mohammadi
Keyword(s):  
Molecular Identification ◽  
Candida Species ◽  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Etiologic Agent ◽  
Molecular Techniques ◽  
University Hospital ◽  
Medical Mycology ◽  
Clinical Samples ◽  
Pcr Multiplex

Abstract Objective: Yeasts are opportunistic microorganisms can cause human fungal infection among immunocompromised patients. This study aimed to identify Candida species and uncommon yeasts obtained from clinical specimens in Kashani university hospital and Shefa Lab as a referral medical mycology laboratory, in Isfahan, Iran, by combination of various molecular techniques. Results: A total of 202 yeast strains were isolated from 341 clinical samples between February 2017 to May 2019. All clinical isolates were identified using phenotypic and molecular tests. PCR-RFLP, duplex-PCR, multiplex-PCR, and PCR-sequencing were applied for molecular identification of yeasts. The most clinical samples were obtained from urine (66.8%), nail (9.4%), bronchoalveolar lavage (5.9%), sore (4.4%), and blood (3.9%). One hundred and twenty-one Candida species were identified as non- albicans against 76 Candida albicans. Trichosporon asahii, and Pichia terricola were uncommon non- Candida yeasts isolated from urine samples. For the first time, we isolated P. terricola as etiologic agent of urinary tract infection in a pregnant female. Since non- albicans Candida species and non- Candida yeasts have various virulence factors and antifungal susceptibility profile, precise molecular identification can help us to reach to the advantageous strategies for treatment of these fungal infections.

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