scholarly journals Outdoor Residual Insecticide Spraying (ODRS), a New Approach for the Control of the Exophilic Vectors of Human Visceral Leishmaniasis: Phlebotomus orientalis in East Africa

2020 ◽  
Vol 14 (10) ◽  
pp. e0008774
Author(s):  
Dia-Eldin A. Elnaiem ◽  
Osman Dakein ◽  
Ahmed Mohammed-Ali Alawad ◽  
Bashir Alsharif ◽  
Altayeb Khogali ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
East Africa ◽  
New Approach ◽  
Human Visceral Leishmaniasis ◽  
Phlebotomus Orientalis

scholarly journals Dispersal of Lutzomyia longipalpis and expansion of canine and human visceral leishmaniasis in São Paulo State, Brazil

Acta Tropica ◽  
2016 ◽  
Vol 164 ◽  
pp. 233-242 ◽  
Author(s):  
Agda Maria Oliveira ◽  
Carolina Portugal Vieira ◽  
Margareth Regina Dibo ◽  
Marluci Monteiro Guirado ◽  
Lilian Aparecida Colebrusco Rodas ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Sao Paulo ◽  
Lutzomyia Longipalpis ◽  
São Paulo ◽  
Paulo State ◽  
Human Visceral Leishmaniasis ◽  
São Paulo State

scholarly journals Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera

1989 ◽  
Vol 84 (3) ◽  
pp. 309-314 ◽  
Author(s):  
M. G. Morgado ◽  
J. Ivo-dos-Santos ◽  
R. T. Pinho ◽  
E. Argüelles ◽  
J. M. Rezende ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Trypanosoma Cruzi ◽  
Western Blot ◽  
Chagas Disease ◽  
Cross Reactivity ◽  
Cross Reaction ◽  
The Other ◽  
Molecular Weights ◽  
Human Visceral Leishmaniasis ◽  
Human Immune

Soluble antigens from epimastigotes of Trypanosoma cruzi were analyzed by western blot in terms of their reactivity with sera from patients with Chagas' disease. In addition, sera from patients with visceral (AVL) and tegumentar leishmaniasis (ATL) were also tested in order to identify cross-reactivities with Trypanosoma cruzy antigens. Twenty eight polypeptides with molecular weights ranging from 14 kDa to 113 kDa were identified with sera from Chagas' disease patients. An extensive cross-reactivity was observed when sera from human visceral leishmaniasis were used, while only a slight cross-reaction was observed with sera from tegumentar leishmaniasis. On the other hand, 10 polypeptidesspecifically reacting with sera from Chagas' disease patients were identified. Among them, the antigens with molecular weights of 46 kDa and 25 kDa reacted with all sera teste and may be good candidates for specific immunodiagnosis of Chagas' disease.

Characterization of breeding sites of Phlebotomus orientalis – The vector of visceral leishmaniasis in northwestern Ethiopia

Acta Tropica ◽  
2014 ◽  
Vol 139 ◽  
pp. 5-14 ◽  
Author(s):  
Aviad Moncaz ◽  
Oscar Kirstein ◽  
Araya Gebresellassie ◽  
Wossenseged Lemma ◽  
Solomon Yared ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Breeding Sites ◽  
Phlebotomus Orientalis

Interstitial pneumonitis in human visceral leishmaniasis

1989 ◽  
Vol 83 (1) ◽  
pp. 73-76 ◽  
Author(s):  
M.I.S. Duarte ◽  
V.L.R. da Matta ◽  
C.E.P. Corbett ◽  
M.D. Laurenti ◽  
R. Chebabo ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Interstitial Pneumonitis ◽  
Human Visceral Leishmaniasis

scholarly journals Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Daniel Moreira de Avelar ◽  
Débora Moreira Carvalho ◽  
Ana Rabello
Keyword(s):  
Visceral Leishmaniasis ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Health Concern ◽  
Serological Tests ◽  
Blood Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Human Visceral Leishmaniasis ◽  
Highly Sensitive ◽  
Sensitivity Specificity

Visceral leishmaniasis (VL) is considered a major public health concern in Brazil and several regions of the world. A recent advance in the diagnosis of infectious diseases was the development of loop-mediated isothermal amplification (LAMP). The aim of this study was to develop and evaluate a new LAMP assay for detection of K26 antigen-coding gene of L. donovani complex. A total of 219 blood samples of immunocompetent patients, including 114 VL cases and 105 non-VL cases, were analyzed for the diagnosis of VL in the present study. Diagnostic accuracy was calculated against a combination of parasitological and/or serological tests as a reference standard. The results were compared to those of kDNA Leishmania-PCR. The detection limit for the K26-Lamp assay was 1fg L. infantum purified DNA and 100 parasites/mL within 60 min of amplification time with visual detection for turbidity. The assay was specific for L. donovani complex. Sensitivity, specificity, and accuracy were 98.2%, 98.1%, and 98.2%, respectively, for K26-LAMP and 100%, 100%, and 100%, respectively, for kDNA Leishmania-PCR. Excellent agreement was observed between K26-LAMP and kDNA Leishmania-PCR assays (K = 0.96). A highly sensitive and specific LAMP assay targeting K26 antigen-coding gene of L. donovani complex was developed for diagnosis in peripheral blood samples of VL patients.

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