scholarly journals Immature Dendritic Cells Generated from Cryopreserved Human Monocytes Show Impaired Ability to Respond to LPS and to Induce Allogeneic Lymphocyte Proliferation

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e71291 ◽  
Author(s):  
Guilherme Ferreira Silveira ◽  
Pryscilla Fanini Wowk ◽  
Anália Maria Breckenfeld Machado ◽  
Claudia Nunes Duarte dos Santos ◽  
Juliano Bordignon
Keyword(s):  
Dendritic Cells ◽  
Lymphocyte Proliferation ◽  
Human Monocytes ◽  
Impaired Ability ◽  
Immature Dendritic Cells ◽  
Allogeneic Lymphocyte

scholarly journals Characterization of a Phenotypically Unique Population of CD13+ Dendritic Cells Resident in the Spleen

2006 ◽  
Vol 13 (9) ◽  
pp. 1064-1069 ◽  
Author(s):  
Yan Zhuang ◽  
Waithaka Mwangi ◽  
Wendy C. Brown ◽  
William C. Davis ◽  
Jayne C. Hope ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Peripheral Blood ◽  
Lymphocyte Proliferation ◽  
Immature Dendritic Cells ◽  
Recall Antigen ◽  
Allogeneic Lymphocyte ◽  
Afferent Lymph ◽  
Stimulation Of

ABSTRACT Immature dendritic cells (DCs) resident in bovine spleens represent a distinct CD11a+ CD11c+ CD13+ CD172+ CD205+ population compared to those circulating in peripheral blood or trafficking via afferent lymph. Upon cytokine-induced maturation, splenic DCs both efficiently present antigen in the stimulation of allogeneic lymphocyte proliferation and recall antigen-specific responses.

Is mannan-binding lectin (MBL) detectable on monocytes and monocyte-derived immature dendritic cells?

2008 ◽  
Vol 36 (6) ◽  
pp. 1497-1500 ◽  
Author(s):  
Shirley L. MacDonald ◽  
Ian Downing ◽  
Marc Turner ◽  
David C. Kilpatrick
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Human Monocytes ◽  
Serum Free Medium ◽  
Free Medium ◽  
Facs Analysis ◽  
Serum Free ◽  
Immature Dendritic Cells ◽  
Mannan Binding Lectin ◽  
Binding Lectin

MBL (mannan-binding lectin; also called mannose-binding lectin) is a circulating C-type lectin with a collagen-like region synthesized mainly by the liver. MBL may influence susceptibility to infection in recipients of stem cell transplants, and it has even been suggested that the MBL status of a donor can influence the recipient's susceptibility to post-transplant infections. We have previously reported that MBL can be detected on human monocytes and monocyte-derived dendritic cells, based on detection using biotinylated anti-MBL, suggesting that those cells could synthesize MBL. If true, permanent MBL replacement therapy could be achieved by stem cell infusions. However, two other groups independently failed to find mbl-2-derived mRNA in monocytes. Therefore, to confirm or refute our previous observations, we used an alternative experimental strategy. Instead of using biotinylated antibody and labelled streptavidin, detection of surface MBL was attempted using MBL-specific primary antibodies (131-1, 131-10 and 131-11) followed by fluorescein-labelled anti-IgG, and controlled by the use of non-specific IgG as primary antibody. Monocytes were counterstained with anti-CD14-PE before FACS analysis. Adherent monocytes were also cultured for 48 h in serum-free medium or converted into immature dendritic cells by culture with IL-4 (interleukin-4) and GM-CSF (granulocyte/monocyte colony-stimulating factor). During FACS analysis, the dendritic cells were gated after counter-staining with anti-CD1a-PE. MBL was readily detected on the surface of fresh monocytes using all three specific anti-MBL monoclonal antibodies, but specific anti-MBL binding was greatly diminished after monocytes had been cultured for 2 days in serum-free medium. Moreover, we could not detect any MBL present on the surface of monocyte-derived dendritic cells. We therefore conclude that MBL is indeed present on the surface of fresh human monocytes. However, in view of the mRNA findings of others and our own previous observation that no secretion of MBL took place in culture, we presume that the surface-bound MBL is derived from autologous plasma and not synthesized by the cells. This conclusion is consistent with our in vivo findings in stem cell transplant patients which provided evidence against significant extra-hepatic production of serum MBL. It provides no ready explanation for the remarkable observation of Mullighan, Heatley, Doherty, Szabo, Grigg, Hughes, Schwarer, Szer, Tait, Bik To and Bardy [(2002) Blood 99, 3524–3529] that the presence of variant alleles of mbl-2 in stem cell donors can influence susceptibility to serious infections in their recipients.

scholarly journals Exposure to Bacillus anthracis Capsule Results in Suppression of Human Monocyte-Derived Dendritic Cells

2014 ◽  
Vol 82 (8) ◽  
pp. 3405-3416 ◽  
Author(s):  
Tanya M. Jelacic ◽  
Donald J. Chabot ◽  
Joel A. Bozue ◽  
Steven A. Tobery ◽  
Michael W. West ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Bacillus Anthracis ◽  
Human Monocyte ◽  
Interleukin 4 ◽  
Human Monocytes ◽  
Content Type ◽  
Bacterial Surface ◽  
Surface Marker Expression ◽  
Immature Dendritic Cells ◽  
The Impact

ABSTRACTThe antiphagocytic capsule ofBacillus anthracisis a major virulence factor. We hypothesized that it may also mediate virulence through inhibition of the host's immune responses. During an infection, the capsule exists attached to the bacterial surface but also free in the host tissues. We sought to examine the impact of free capsule by assessing its effects on human monocytes and immature dendritic cells (iDCs). Human monocytes were differentiated into iDCs by interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) over 7 days in the presence of capsule derived from wild-type encapsulatedB. anthracisAmes (WT) or a control preparation from an isogenicB. anthracisAmes strain that produces only 2% of the capsule of the WT (capAmutant). WT capsule consistently induced release of IL-8 and IL-6 while thecapAmutant control preparation elicited either no response or only a minimal release of IL-8. iDCs that were differentiated in the presence of WT capsule had increased side scatter (SSC), a measure of cellular complexity, when assessed by flow cytometry. iDCs differentiated in the presence of WT capsule also matured less well in response to subsequentB. anthracispeptidoglycan (Ba PGN) exposure, with reduced upregulation of the chemokine receptor CCR7, reduced CCR7-dependent chemotaxis, and reduced release of certain cytokines. Exposure of naive differentiated control iDCs to WT capsule did not alter cell surface marker expression but did elicit IL-8. These results indicate that free capsule may contribute to the pathogenesis of anthrax by suppressing the responses of immune cells and interfering with the maturation of iDCs.

Higher CD1a Levels Correlate with PD-L1 Expression and Predict Worse Overall Survival in Triple-Negative Breast Carcinoma

Breast Care ◽  
2021 ◽  
pp. 1-9
Author(s):  
Jian Zheng ◽  
Yuntao Wei ◽  
Xiaoxi Li ◽  
Zhan Shen ◽  
Yong Zhang ◽  
...  
Keyword(s):  
Overall Survival ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Breast Carcinoma ◽  
Triple Negative ◽  
Carcinoma Tissue ◽  
Kaplan Meier ◽  
Immature Dendritic Cells ◽  
Triple Negative Breast Carcinoma ◽  
High Median

Objective: The aim of this study was to measure the expression of PD-L1, CD1a (a marker for immature dendritic cells), and CD83 (a marker for mature dendritic cells) and further examine the associations of PD-L1, CD83, and CD1a with overall survival (OS) in triple-negative breast carcinoma patients. Methods: PD-L1, CD1a, and CD83 expression in breast carcinoma tissues and CD83 expression in lymph node tissues were examined by immunohistochemistry and tissue microarray in 159 patients. Patients were classified into the low, medium, and high PD-L1, CD1a, and CD83 levels. Pearson χ2 test was used to analyze the correlations between PD-L1, CD1a, and CD83. The Kaplan-Meier method was used to calculate the OS. Multivariate analysis was used to identify determinants of 3- and 5-year OS. Results: 25.1, 25.8, and 49.1% of the patients had low, medium, and high PD-L1 levels, respectively. PD-L1 levels significantly correlated with CD1a (r = 0.30409, p < 0.001) and CD83 levels (r = 0.6146, p < 0.001) in breast carcinoma tissue, as well as CD83 levels (r = 0.17508, p = 0.027) in lymph node. The median OS was 83 months (range 12–106), and the 3- and 5-year OS rates were 94.97% (95% CI 91.57–98.37) and 86.79% (95% CI 81.53–92.06), respectively. Moreover, patients with high median CD1a levels had a significantly lower 5-year OS rate (75.6%) than those with low median CD1a levels (93.5%, p = 0.038). Conclusion: PD-L1, CD1a, and CD83 are variably expressed in triple-negative breast carcinoma tissues, and PD-L1 expression correlates with CD1a and CD83. Higher CD1a levels correlate with PD-L1 expression and predict worse OS in triple-negative breast carcinoma.

scholarly journals Effect of serotonin on the differentiation of human monocytes into dendritic cells

2006 ◽  
Vol 146 (2) ◽  
pp. 354-361 ◽  
Author(s):  
N. Katoh ◽  
F. Soga ◽  
T. Nara ◽  
R. Tamagawa-Mineoka ◽  
M. Nin ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Human Monocytes
Sign in / Sign up

Export Citation Format

Share Document