scholarly journals First Evaluation of Drug-Resistant Mycobacterium tuberculosis Clinical Isolates from Congo Revealed Misdetection of Fluoroquinolone Resistance by Line Probe Assay Due to a Double Substitution T80A-A90G in GyrA

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e95083 ◽  
Author(s):  
Alexandra Aubry ◽  
Wladimir Sougakoff ◽  
Pamela Bodzongo ◽  
Guy Delcroix ◽  
Sylvie Armand ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Isolates ◽  
Fluoroquinolone Resistance ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Double Substitution ◽  
Probe Assay

scholarly journals Evaluation of a line probe assay for the rapid detection of gyrA mutations associated with fluoroquinolone resistance in multidrug-resistant Mycobacterium tuberculosis

2011 ◽  
Vol 60 (2) ◽  
pp. 184-188 ◽  
Author(s):  
Hiroki Ando ◽  
Satoshi Mitarai ◽  
Yuji Kondo ◽  
Toshinori Suetake ◽  
Seiya Kato ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Global Health ◽  
Dna Sequencing ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Surveillance Study ◽  
Fluoroquinolone Resistance ◽  
National Surveillance ◽  
Line Probe Assay ◽  
Probe Assay

The aim of this study was to establish the importance of detecting fluoroquinolone (FQ) resistance in multidrug resistant (MDR) Mycobacterium tuberculosis, and to show the usefulness of a hybridization-based line probe assay (LiPA) for detecting gyrA mutations. Thirty-three MDR M. tuberculosis isolates were collected from a total of sixty MDR isolates identified in Japan over 6 months during a national surveillance study in 2002. Seventeen MDR isolates were collected by the National Center for Global Health and Medicine in Japan over 6 years from 2003 to 2008. These 50 isolates were examined for FQ susceptibility, and analysed by LiPA and gyrA sequencing. Among them, 22 (44 %) showed FQ resistance. All FQ-resistant isolates had at least one mutation in gyrA. The results of the LiPA were fully consistent with the DNA sequencing results. Given that on the basis of our results almost half of the MDR M. tuberculosis isolates in Japan might have resistance to FQ, it is important to monitor FQ resistance in patients with MDR tuberculosis (TB), as well as with drug-susceptible TB, prior to commencing treatment. For the detection of FQ resistance, LiPA is useful and can rapidly and efficiently assess FQ resistance.

Assessment of line probe assay for rapid detection of extensively drug-resistant Mycobacterium tuberculosis isolates

2021 ◽  
Vol 9 (5) ◽  
pp. 65
Author(s):  
Jalil Kardan-Yamchi ◽  
Hossein Kazemian ◽  
HamidReza Abtahi ◽  
Sirus Amini ◽  
MohammadMehdi Feizabadi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Extensively Drug Resistant ◽  
Probe Assay

Comparison of rifabutin susceptibility and rpoB mutations in multi-drug-resistant Mycobacterium tuberculosis strains by DNA sequencing and the line probe assay

2010 ◽  
Vol 16 (5) ◽  
pp. 360-363 ◽  
Author(s):  
Shiomi Yoshida ◽  
Katsuhiro Suzuki ◽  
Kazunari Tsuyuguchi ◽  
Masaji Okada ◽  
Motohisa Tomita ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Dna Sequencing ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Probe Assay
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