Multilocus Genotyping of Giardia duodenalis in Dairy Cattle in Henan, China

Giardia duodenalis is an important zoonotic pathogen for both human and animal health. Although there have been reports on G. duodenalis infections in animals all over the world, information regarding the prevalence and genetic characteristics of G. duodenalis in sheep in Inner Mongolia, China, is limited. In this study, 209 sheep fecal specimens were collected in this autonomous region. We established that the prevalence of G. duodenalis was 64.11% (134/209), as determined using nested PCR detection and sequences analysis of the small subunit ribosomal RNA (SSU rRNA) gene. Based on the beta-giardin (bg) locus, the glutamate dehydrogenase (gdh) locus, and the triose phosphate isomerase (tpi) locus to study genetic characteristics, both assemblages A (2.99%, 4/134) and E (97.01%, 130/134) were found. Five novel nucleotide sequence of assemblage E were detected, two at the bg locus, two at the gdh locus, and one at the tpi locus. Multilocus genotyping yielded four assemblage E and two assemblage A multilocus genotypes (MLGs), including four novel assemblage E MLGs and one novel assemblage A MLG. Results of this study indicated that G. duodenalis was highly prevalent in sheep in Inner Mongolia. This study is the first to use the multilocus genotyping approach to identify G. duodenalis in sheep from this region.

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Multilocus genotyping of Giardia duodenalis isolated from patients in Egypt

Acta Tropica ◽

10.1016/j.actatropica.2019.05.012 ◽

2019 ◽

Vol 196 ◽

pp. 66-71 ◽

Cited By ~ 2

Author(s):

Fuchang Yu ◽

Said Amer ◽

Meng Qi ◽

Rongjun Wang ◽

Yange Wang ◽

...

Keyword(s):

Giardia Duodenalis ◽

Multilocus Genotyping

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Multilocus genotyping of Giardia duodenalis from pigs in Korea

Parasitology International ◽

10.1016/j.parint.2020.102154 ◽

2020 ◽

Vol 78 ◽

pp. 102154 ◽

Cited By ~ 1

Author(s):

Haeseung Lee ◽

ByeongYeal Jung ◽

Jun-Sik Lim ◽

Min-Goo Seo ◽

Seung-Hun Lee ◽

...

Keyword(s):

Giardia Duodenalis ◽

Multilocus Genotyping

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Multilocus genotyping and risk factor analysis of Giardia duodenalis in dogs in Korea

Acta Tropica ◽

10.1016/j.actatropica.2019.105113 ◽

2019 ◽

Vol 199 ◽

pp. 105113 ◽

Cited By ~ 6

Author(s):

Ha-Young Kim ◽

Haeseung Lee ◽

Seung-Hun Lee ◽

Min-Goo Seo ◽

Seonju Yi ◽

...

Keyword(s):

Factor Analysis ◽

Risk Factor ◽

Giardia Duodenalis ◽

Risk Factor Analysis ◽

Multilocus Genotyping

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Prevalence and multilocus genotyping of Giardia duodenalis in dairy calves in Xinjiang, Northwestern China

Parasites & Vectors ◽

10.1186/s13071-016-1828-3 ◽

2016 ◽

Vol 9 (1) ◽

Cited By ~ 14

Author(s):

Meng Qi ◽

Haiyan Wang ◽

Bo Jing ◽

Rongjun Wang ◽

Fuchun Jian ◽

...

Keyword(s):

Giardia Duodenalis ◽

Dairy Calves ◽

Northwestern China ◽

Multilocus Genotyping

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Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey

PLoS ONE ◽

10.1371/journal.pone.0228317 ◽

2020 ◽

Vol 15 (2) ◽

pp. e0228317

Author(s):

Abdollah Rafiei ◽

Raheleh Baghlaninezhad ◽

Pamela C. Köster ◽

Begoña Bailo ◽

Marta Hernández de Mingo ◽

...

Keyword(s):

Giardia Duodenalis ◽

Community Survey ◽

Multilocus Genotyping ◽

Southwestern Iran

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Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Gansu, northwest China

Parasite ◽

10.1051/parasite/2020058 ◽

2020 ◽

Vol 27 ◽

pp. 62

Author(s):

Yilin Wang ◽

Jianke Cao ◽

Yankai Chang ◽

Fuchang Yu ◽

Sumei Zhang ◽

...

Keyword(s):

Dairy Cattle ◽

Ribosomal Rna ◽

Triosephosphate Isomerase ◽

Giardia Duodenalis ◽

Northwest China ◽

Small Subunit ◽

Gastrointestinal Parasites ◽

Rrna Gene ◽

And Control

Cryptosporidium spp. and Giardia duodenalis are common gastrointestinal parasites with a broad range of hosts, including humans, livestock, and wildlife. To examine the infection status and assess the zoonotic potential of Cryptosporidium spp. and G. duodenalis in dairy cattle in Gansu, China, a total of 1414 fecal samples were collected from the rectum, with one sample collected from each individual animal. All the samples were tested using nested PCR based on the small subunit ribosomal RNA (SSU rRNA) gene of Cryptosporidium spp. and G. duodenalis. The overall infection rates of Cryptosporidium spp. and Giardia duodenalis were 4.2% (n = 59) and 1.0% (n = 14), respectively. Four Cryptosporidium species were identified: C. andersoni (n = 42), C. parvum (n = 12), C. bovis (n = 5), and C. ryanae (n = 1). In further analyses of subtypes of C. parvum isolates based on the 60 kDa glycoprotein (gp60) gene, five were successfully subtyped as IIdA19G1 (n = 4) and IIdA15G1 (n = 1). All 14 G. duodenalis isolates were identified as assemblage E using the triosephosphate isomerase (tpi) gene. The relatively low positive rates of Cryptosporidium spp. and G. duodenalis detected here and the predominance of non-human pathogenic species/assemblages of these parasites indicated their unique transmission dynamics in this area and the low level of threat posed to public health. However, continuous monitoring and further studies of these parasites should be conducted for the prevention and control of these pathogens.

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