scholarly journals Atomic Force Microscopy Reveals a Morphological Differentiation of Chromobacterium violaceum Cells Associated with Biofilm Development and Directed by N-Hexanoyl-L-Homoserine Lactone

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e103741 ◽  
Author(s):  
Anara A. Kamaeva ◽  
Alexey S. Vasilchenko ◽  
Dmitry G. Deryabin
Keyword(s):  
Atomic Force Microscopy ◽  
Morphological Differentiation ◽  
Homoserine Lactone ◽  
Chromobacterium Violaceum ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biofilm Development

scholarly journals Biofilm Cohesiveness Measurement Using a Novel Atomic Force Microscopy Methodology

2007 ◽  
Vol 73 (9) ◽  
pp. 2897-2904 ◽  
Author(s):  
Francois Ahimou ◽  
Michael J. Semmens ◽  
Paige J. Novak ◽  
Greg Haugstad
Keyword(s):  
Atomic Force Microscopy ◽  
Cohesive Energy ◽  
Waste Treatment ◽  
Energy Levels ◽  
Medical Implants ◽  
Force Microscopy ◽  
Atomic Force ◽  
Energy Dissipated ◽  
Biofilm Development

ABSTRACT Biofilms can be undesirable, as in those covering medical implants, and beneficial, such as when they are used for waste treatment. Because cohesive strength is a primary factor affecting the balance between growth and detachment, its quantification is essential in understanding, predicting, and modeling biofilm development. In this study, we developed a novel atomic force microscopy (AFM) method for reproducibly measuring, in situ, the cohesive energy levels of moist 1-day biofilms. The biofilm was grown from an undefined mixed culture taken from activated sludge. The volume of biofilm displaced and the corresponding frictional energy dissipated were determined as a function of biofilm depth, resulting in the calculation of the cohesive energy. Our results showed that cohesive energy increased with biofilm depth, from 0.10 ± 0.07 nJ/μm3 to 2.05 ± 0.62 nJ/μm3. This observation was reproducible, with four different biofilms showing the same behavior. Cohesive energy also increased from 0.10 ± 0.07 nJ/μm3 to 1.98 ± 0.34 nJ/μm3 when calcium (10 mM) was added to the reactor during biofilm cultivation. These results agree with previous reports on calcium increasing the cohesiveness of biofilms. This AFM-based technique can be performed with available off-the-shelf instrumentation. It could therefore be widely used to examine biofilm cohesion under a variety of conditions.

Correlation betweenEnterococcus faecalisBiofilms Development Stage and Quantitative Surface Roughness Using Atomic Force Microscopy

2008 ◽  
Vol 14 (2) ◽  
pp. 150-158 ◽  
Author(s):  
Ricardo P. Santos ◽  
Theodora T.P. Arruda ◽  
Cibele B.M. Carvalho ◽  
Victor A. Carneiro ◽  
Lara Q.V. Braga ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Brain Heart Infusion ◽  
Development Stage ◽  
Solid Surfaces ◽  
Bacterial Surface ◽  
Force Microscopy ◽  
Atomic Force ◽  
Pollutant Transformation ◽  
Biofilm Development

Biofilms are assemblages of microorganisms and their associated extracellular products at an interface and typically with an abiotic or biotic surface. The study of the morphology of biofilms is important because they are associated with processes of biofouling, corrosion, catalysis, pollutant transformation, dental caries, drug resistance, and so forth. In the literature, biofilms have been examined by atomic force microscopy (AFM), which has proven to be a potent tool to study different aspects of the biofilm development on solid surfaces. In this work, we used AFM to investigate topographical changes during the development process ofEnterococcus faecalisbiofilms, which were generated on sterile cellulose nitrate membrane (CNM) filters in brain heart infusion (BHI) broth agar blood plates after 24, 36, 72, 192, and 360 h. AFM height images showed topographical changes due to biofilm development, which were used to characterize several aspects of the bacterial surface, such as the presence of extracellular polymeric substance, and the biofilm development stage. Changes in the development stage of the biofilm were shown to correlate with changes in the surface roughness as quantified through the mean roughness.

Electrostatic Forces on the Surface of Metals as Measured by Atomic Force Microscopy

2000 ◽  
Vol 10 (1-2) ◽  
pp. 15
Author(s):  
Eugene Sprague ◽  
Julio C. Palmaz ◽  
Cristina Simon ◽  
Aaron Watson
Keyword(s):  
Atomic Force Microscopy ◽  
Electrostatic Forces ◽  
Force Microscopy ◽  
Atomic Force

In Operando Detection of the Physical Properties Change of the Interfacial Electrolyte during Li-Metal Electrode Reaction by Atomic Force Microscopy

2020 ◽  
Author(s):  
Mitsunori Kitta
Keyword(s):  
Atomic Force Microscopy ◽  
Energy Dissipation ◽  
Physical Properties ◽  
Electrode Surface ◽  
Electrode Reaction ◽  
Metal Electrode ◽  
Ion Concentration ◽  
Force Microscopy ◽  
Atomic Force ◽  
Discharge Reaction

This manuscript propose the operando detection technique of the physical properties change of electrolyte during Li-metal battery operation.The physical properties of electrolyte solution such as viscosity (η) and mass densities (ρ) highly affect the feature of electrochemical Li-metal deposition on the Li-metal electrode surface. Therefore, the operando technique for detection these properties change near the electrode surface is highly needed to investigate the true reaction of Li-metal electrode. Here, this study proved that one of the atomic force microscopy based analysis, energy dissipation analysis of cantilever during force curve motion, was really promising for the direct investigation of that. The solution drag of electrolyte, which is controlled by the physical properties, is directly concern the energy dissipation of cantilever motion. In the experiment, increasing the energy dissipation was really observed during the Li-metal dissolution (discharge) reaction, understanding as the increment of η and ρ of electrolyte via increasing of Li-ion concentration. Further, the dissipation energy change was well synchronized to the charge-discharge reaction of Li-metal electrode.This study is the first report for direct observation of the physical properties change of electrolyte on Li-metal electrode reaction, and proposed technique should be widely interesting to the basic interfacial electrochemistry, fundamental researches of solid-liquid interface, as well as the battery researches.

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