Identification and Characterization of Circular RNAs As a New Class of Putative Biomarkers in Human Blood

Mycobacterium marinum, a close relative of the significant human pathogen Mycobacterium tuberculosis, polymerizes host actin at the bacterial surface to drive intracellular movement and cell-to-cell spread during infection. Here, we report the identification and characterization of MirA, the M. marinum actin-based motility factor. MirA is a member of the glycine-rich PE_PGRS family of ESX-5-secreted proteins. MirA uses an amphipathic helix to anchor into the mycobacterial outer membrane and, surprisingly, also the surface of host lipid droplet organelles. The glycine-rich PGRS domain in MirA directly binds and activates host N-WASP to stimulate actin polymerization through the Arp2/3 complex, directing both bacterial and lipid droplet actin-based motility. MirA is dissimilar to known N-WASP activating ligands and may represent a new class of microbial and host actin regulator. Additionally, the MirA-N-WASP interaction represents a model to understand how the enigmatic PE_PGRS proteins contribute to mycobacterial pathogenesis.

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Identification and characterization of circular RNAs in chicken hepatocytes

Growth Hormone & IGF Research ◽

10.1016/j.ghir.2019.05.002 ◽

2019 ◽

Vol 46-47 ◽

pp. 16-23 ◽

Cited By ~ 1

Author(s):

Li Zhang ◽

Haidong Xu ◽

Zhang Wang ◽

Ting Li ◽

Jiali Guo ◽

...

Keyword(s):

Circular Rnas ◽

Identification And Characterization

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Identification and Characterization of a New Class of (6–4) Photolyase from Vibrio cholerae

Biochemistry ◽

10.1021/acs.biochem.9b00766 ◽

2019 ◽

Vol 58 (43) ◽

pp. 4352-4360 ◽

Cited By ~ 4

Author(s):

Ugur Meric Dikbas ◽

Mehmet Tardu ◽

Asena Canturk ◽

Seref Gul ◽

Gozde Ozcelik ◽

...

Keyword(s):

Vibrio Cholerae ◽

New Class ◽

Identification And Characterization

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Genome-wide identification and characterization of circular RNAs by high throughput sequencing in soybean

Scientific Reports ◽

10.1038/s41598-017-05922-9 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 46

Author(s):

Wei Zhao ◽

Yihui Cheng ◽

Chi Zhang ◽

Qingbo You ◽

Xinjie Shen ◽

...

Keyword(s):

High Throughput ◽

High Throughput Sequencing ◽

Circular Rnas ◽

Genome Wide ◽

Identification And Characterization

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Identification and Characterization of Rice Circular RNAs Responding to Xanthomonas Oryzae pv. Oryzae Invasion

10.21203/rs.3.rs-164815/v1 ◽

2021 ◽

Author(s):

Peihong Wang ◽

Sai Wang ◽

Yan Wu ◽

Wenhan Nie ◽

Ayizekeranmu Yiming ◽

...

Keyword(s):

Molecular Mechanisms ◽

Expression Profiles ◽

Circular Rnas ◽

Pathogen Invasion ◽

Rice Leaves ◽

Transcriptional Gene Regulation ◽

Identification And Characterization ◽

Host Genes

Abstract BackgroundThe emerging role of circular RNAs (circRNAs) in various biological processes have advanced our knowledge of transcriptional and post-transcriptional gene regulation. The number and expression of plant circRNAs vary with species and treatments. However, the expression profile and the potential role of circRNAs during plant response to pathogen invasion are still elusive. ResultsIn this study, we identified 3517 circRNAs from PXO99A-infected rice leaves using the ribosomal RNA (rRNA) depleted RNA-Sequencing technique coupled with the CIRI2 and CIRCexplorer2 pipeline. Among them, 2994 (85.13%) circRNAs arised from the exons of their parent genes, 1214 circRNAs were previously unknown and 276 circRNAs exhibited differential expression profiles upon PXO99A infection over time. In addition, 31 differentially expressed circRNAs (DEcircRNAs) were predicted as the corresponding 121 miRNAs sponges. Functional analysis of both host genes and target mRNAs suggested that these identified circRNAs might play an important role in reprogramming rice responses to PXO99A invasion, mainly by mediating photorespiration, chloroplast, peroxisome and diterpenoid biosynthesis associated pathways.ConclusionThese results inferred a potential functional role of circRNAs in the regulation of rice immunity and provide novel clues for revealing the molecular mechanisms of rice-PXO99A interaction.

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Long read sequencing reveals a novel class of structural aberrations in cancers: identification and characterization of cancerous local amplifications

10.1101/620047 ◽

2019 ◽

Cited By ~ 5

Author(s):

Yoshitaka Sakamoto ◽

Liu Xu ◽

Masahide Seki ◽

Toshiyuki T. Yokoyama ◽

Masahiro Kasahara ◽

...

Keyword(s):

Point Mutations ◽

Lung Cancers ◽

New Class ◽

Large Deletions ◽

Long Read ◽

Structural Aberrations ◽

Molecular Etiology ◽

Identification And Characterization

AbstractHere we report identification of a new class of local structural aberrations in lung cancers. The whole-genome sequencing of cell lines using a long read sequencer, PromethION, demonstrated that typical cancerous mutations, such as point mutations, large deletions and gene fusions can be detected also on this platform. Unexpectedly, we revealed unique structural aberrations consisting of complex combinations of local duplications, inversions and micro deletions. We further analyzed and found that these mutations also occur in vivo, even in key cancer-related genes. These mutations may elucidate the molecular etiology of patients for whom causative cancerous events and therapeutic strategies remain elusive.

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