scholarly journals A mycobacterial glycine-rich protein governs actin-based motility

2021 ◽  
Author(s):  
Norbert S. Hill ◽  
Matthew D. Welch
Keyword(s):  
Lipid Droplet ◽  
Actin Polymerization ◽  
Close Relative ◽  
Bacterial Surface ◽  
Motility Factor ◽  
New Class ◽  
Intracellular Movement ◽  
Mycobacterial Pathogenesis ◽  
Identification And Characterization

Mycobacterium marinum, a close relative of the significant human pathogen Mycobacterium tuberculosis, polymerizes host actin at the bacterial surface to drive intracellular movement and cell-to-cell spread during infection. Here, we report the identification and characterization of MirA, the M. marinum actin-based motility factor. MirA is a member of the glycine-rich PE_PGRS family of ESX-5-secreted proteins. MirA uses an amphipathic helix to anchor into the mycobacterial outer membrane and, surprisingly, also the surface of host lipid droplet organelles. The glycine-rich PGRS domain in MirA directly binds and activates host N-WASP to stimulate actin polymerization through the Arp2/3 complex, directing both bacterial and lipid droplet actin-based motility. MirA is dissimilar to known N-WASP activating ligands and may represent a new class of microbial and host actin regulator. Additionally, the MirA-N-WASP interaction represents a model to understand how the enigmatic PE_PGRS proteins contribute to mycobacterial pathogenesis.

scholarly journals Identification and Characterization of Circular RNAs as a New Class of Putative Biomarkers in Diabetes Retinopathy

2017 ◽  
Vol 58 (14) ◽  
pp. 6500 ◽  
Author(s):  
Shu-Jie Zhang ◽  
Xue Chen ◽  
Chao-Peng Li ◽  
Xiu-Miao Li ◽  
Chang Liu ◽  
...  
Keyword(s):  
Circular Rnas ◽  
New Class ◽  
Diabetes Retinopathy ◽  
Identification And Characterization

scholarly journals Characterization of a new class of blue-fluorescent lipid droplet markers for live-cell imaging in plants

2015 ◽  
Vol 34 (4) ◽  
pp. 655-665 ◽  
Author(s):  
Soujanya Kuntam ◽  
László G. Puskás ◽  
Ferhan Ayaydin
Keyword(s):  
Lipid Droplet ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
New Class ◽  
Fluorescent Lipid

scholarly journals Identification and Characterization of a New Class of Trafficking Motifs for Controlling Clathrin-independent Internalization and Recycling

2007 ◽  
Vol 282 (17) ◽  
pp. 13087-13097 ◽  
Author(s):  
Qiang Gong ◽  
Michael Weide ◽  
Christopher Huntsman ◽  
Zhuojin Xu ◽  
Lily Y. Jan ◽  
...  
Keyword(s):  
New Class ◽  
Identification And Characterization

Identification and Characterization of a New Class of (6–4) Photolyase from Vibrio cholerae

Biochemistry ◽  
2019 ◽  
Vol 58 (43) ◽  
pp. 4352-4360 ◽  
Author(s):  
Ugur Meric Dikbas ◽  
Mehmet Tardu ◽  
Asena Canturk ◽  
Seref Gul ◽  
Gozde Ozcelik ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
New Class ◽  
Identification And Characterization

scholarly journals Long read sequencing reveals a novel class of structural aberrations in cancers: identification and characterization of cancerous local amplifications

2019 ◽  
Author(s):  
Yoshitaka Sakamoto ◽  
Liu Xu ◽  
Masahide Seki ◽  
Toshiyuki T. Yokoyama ◽  
Masahiro Kasahara ◽  
...  
Keyword(s):  
Point Mutations ◽  
Lung Cancers ◽  
New Class ◽  
Large Deletions ◽  
Long Read ◽  
Structural Aberrations ◽  
Molecular Etiology ◽  
Identification And Characterization

AbstractHere we report identification of a new class of local structural aberrations in lung cancers. The whole-genome sequencing of cell lines using a long read sequencer, PromethION, demonstrated that typical cancerous mutations, such as point mutations, large deletions and gene fusions can be detected also on this platform. Unexpectedly, we revealed unique structural aberrations consisting of complex combinations of local duplications, inversions and micro deletions. We further analyzed and found that these mutations also occur in vivo, even in key cancer-related genes. These mutations may elucidate the molecular etiology of patients for whom causative cancerous events and therapeutic strategies remain elusive.

scholarly journals Identification and Characterization of Circular RNAs As a New Class of Putative Biomarkers in Human Blood

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141214 ◽  
Author(s):  
Sebastian Memczak ◽  
Panagiotis Papavasileiou ◽  
Oliver Peters ◽  
Nikolaus Rajewsky
Keyword(s):  
Human Blood ◽  
Circular Rnas ◽  
New Class ◽  
Identification And Characterization

scholarly journals Fish Monocytes as a Model for Mycobacterial Host-Pathogen Interactions

2001 ◽  
Vol 69 (12) ◽  
pp. 7310-7317 ◽  
Author(s):  
Sahar H. El-Etr ◽  
Ling Yan ◽  
Jeffrey D. Cirillo
Keyword(s):  
Mycobacterium Smegmatis ◽  
Virulence Determinants ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Monocytic Cells ◽  
Vitro Model ◽  
Mycobacterial Pathogenesis ◽  
Identification And Characterization

ABSTRACT Mycobacterium marinum, a relatively rapid-growing fish and human pathogen, has become an important model for the investigation of mycobacterial pathogenesis. M. marinumis closely related to the Mycobacterium tuberculosiscomplex and causes a disease in fish and amphibians with pathology similar to tuberculosis. We have developed an in vitro model for the study of M. marinum virulence mechanisms using the carp monocytic cell line CLC (carp leukocyte culture). We found that fish monocytes can differentiate between pathogenic and nonpathogenic mycobacterial species. Interestingly, M. marinum enters fish monocytes at a 40- to 60-fold-higher rate thanMycobacterium smegmatis. In addition, M. marinum survives and replicates in fish monocytes whileM. smegmatis is killed. We also found that M. marinum inhibits lysosomal fusion in fish monocytes, indicating that these cells may be used to dissect the mechanisms of intracellular trafficking in mycobacteria. We conclude from these observations that monocytic cells from fish, a natural host for M. marinum, provide an extremely valuable model for the identification and characterization of mycobacterial virulence determinants in the laboratory.

scholarly journals Identification and Characterization of Associated with Lipid Droplet Protein 1: A Novel Membrane-Associated Protein That Resides on Hepatic Lipid Droplets

Traffic ◽  
2006 ◽  
Vol 7 (9) ◽  
pp. 1254-1269 ◽  
Author(s):  
Silvia Turró ◽  
Mercedes Ingelmo-Torres ◽  
Josep M. Estanyol ◽  
Francesc Tebar ◽  
Manuel A. Fernández ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Droplets ◽  
Hepatic Lipid ◽  
Identification And Characterization ◽  
Lipid Droplet Protein
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